| 刘宏伟,王玉华.慢性乙型肝炎病人外周血单个核细胞内微小 RNA-155、核苷酸结合寡聚化结构域样 3表达与乙型肝炎病毒 DNA载量的相关性[J].安徽医药,2021,25(2):373-377. |
| 慢性乙型肝炎病人外周血单个核细胞内微小 RNA-155、核苷酸结合寡聚化结构域样 3表达与乙型肝炎病毒 DNA载量的相关性 |
| Correlations between the expressions of Mir-155, NLRP3 in peripheral blood and HBV-DNA load in patients with chronic hepatitis B |
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| DOI：10.3969/j.issn.1009-6469.2021.02.039. |
| 中文关键词: 乙型肝炎，慢性 乙型肝炎病毒 微小 RNA-155 核苷酸结合寡聚化域 3 |
| 英文关键词: Hepatitis B, Chronic Hepatitis B virus microRNA-155 Nucleotide-binding oligomerization domain 3 |
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| 中文摘要: |
| 目的研究慢性乙型肝炎( CHB)病人外周血单个核细胞( PBMC)内微小 RNA-155(Mir-155)、核苷酸结合寡聚化结构域样 3(NLRP3)表达与乙型肝炎病毒 DNA(HBV-DNA)载量的相关性。方法选取 2017年 6月至 2019年 9月沧州市传染病医院收治的经肝脏穿刺病理确诊的 CHB病人 118例为研究对象，根据荧光定量聚合酶链式反应( RT-qPCR)检测 HBV-DNA载量分为低载量组( HBV-DNA < 1×105拷贝 /毫升) 51例，中载量组( 1×105拷贝 /毫升 ≤ HBV-DNA1×107拷贝 /毫升) 38例，高载量组(HBV-DNA > 1×107拷贝 /毫升) 29例，另选取同期门诊健康体检者 118例为健康对照组。采用 RT-qPCR检测两组 PBMC内 Mir155、NLRP3 mRNA及蛋白表达水平，采用酶联免疫吸附试验( ELISA)检测血清白细胞介素 -1β(IL-1β)、白细胞介素 -18(IL-18)水平，采用 Pearson法分析 CHB病人 Mir-155与 NLRP3蛋白水平及二者分别与 IL-1β、IL-18水平的相关性。结果与健康对照组比较， CHB病人血清肝功能指标天门冬氨酸氨基转移酶(AST)［(18.17±3.65)mmol/L比( 52.06±10.57)mmol/L］、丙氨酸氨基转移酶( ALT)［( 22.84±4.56)mmol/L比( 58.93±11.74)mmol/L］、 PBMC中 Mir-155［( 1.01±0.16)比( 3.42±0.53)］、 NLRP3 mRNA［( 1.02±0.15)比( 4.26±0.75)］水平均显著升高( P < 0.05)。与 HBV-DNA低载量组比较，中、高载量组 CHB病人 PBMC中 Mir155、NLRP3 mRNA及蛋白水平、血清 IL-1β、IL-18水平依次增加( P < 0.05)。随肝炎炎症分级增加、肝纤维化分期增加， PBMC中 Mir-155、NLRP3 mRNA水平依次增加(均 P < 0.05)。 CHB病人 PBMC中 Mir-155与 NLRP3、IL-1β、IL-18水平均呈显著正相关(r = 0.714、0.733、0.852，P < 0.05)NLRP3水平与血清 IL-1β、IL-18水平均呈显著正相关( r 0.685、0.812，P < 0.05)。结论随HBV-DNA载量增加， CHB病人 Mir-1，55、NLRP3水平增加，可能协同促进炎症反应影响 CHB发生发展。 |
| 英文摘要: |
| Objective To study the correlations between the expressions of microRNA-155 (Mir-155), nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) in peripheral blood mononuclear cells (PBMC) and the load of hepatitis B virus gene (HBV-DNA) of patients with chronic hepatitis B (CHB).Methods One hundred and eighteen patients with CHB who were diagnosedby liver biopsy from June 2017 to September 2019 were selected as the study subjects. According to the detection of HBV-DNA load by fluorescence quantitative PCR (RT-qPCR), 51 cases were assigned into low-load group (HBV-DNA < 1×105 copies/mL), 38 cases in me?dium-load group (1×105 copies/mL < HBV-DNA < 1×107 copies/mL), 29 cases in high-load group (HBV-DNA > 1×107copies/mL),and another 118 healthy outpatients in the same period were selected as control group. The levels of Mir-155 and NLRP3 in PBMC were detected by RT-qPCR, the levels of IL-1β and IL-18 in serum were detected by ELISA, and the levels of Mir-155 and NLRP3 in CHB patients were analyzed by Pearson method.Results Compared with the control group, the serum levels of AST [(18.17±3.65)mmol/L vs. (52.06±10.57)mmol/L],ALT [(22.84±4.56)mmol/L vs. (58.93±11.74)mmol/L] and the levels Mir-155 [(1.01±0.16) vs. (3.42±0.53)], NLRP3 mRNA [(1.02±0.15) vs. (4.26±0.75)] in PBMC in CHB patients were significantly higher (P < 0.05). Compared with HBV-DNA low-load group, the levels of Mir-155, NLRP3 mRNAs and proteins, IL-1β and IL-18 in PBMC of CHB patients in medium and high-load groups increased in turn (P < 0.05). The mRNA levels of Mir-155 and NLRP3 in PBMC increased successively with the increase of hepatitis inflammation grade and liver fibrosis stage (P < 0.05). Mir-155 in PBMC of CHB patients was positively correlated with NLRP3, IL-1β and IL-18 levels (r= 0.714,0.733,0.852,P < 0.05), and the level of NLRP3 was positively correlated with the levels of serum IL-1β and IL-18 (r= 0.685,0.812,P < 0.05).Conclusion With the increase of HBV-DNA load, the levels of Mir-155 and NLRP3 in CHB patients increase, which may synergistically promote inflammation and affect the occurrence and development of CHB. |
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