| 闫书娜,马腾,李国锋,等.微小 RNA-194-5p与 cullin4A在肺结核病人中的表达及意义[J].安徽医药,2021,25(3):489-493. |
| 微小 RNA-194-5p与 cullin4A在肺结核病人中的表达及意义 |
| Expression and significance of miR-194-5p and CUL4A in patients with tuberculosis |
| |
| DOI：10.3969/j.issn.1009-6469.2021.03.014 |
| 中文关键词: 结核，肺 结核分枝杆菌 微小 RNA-194-5p 泛素蛋白连接酶类 cullin4A 炎症 |
| 英文关键词: Tuberculosis, pulmonary Mycobacterium tuberculosis MiR-194-5p Ubiquitin-protein ligases CUL4A Inflammation |
| 基金项目: |
|
| 摘要点击次数: 2068 |
| 全文下载次数: 706 |
| 中文摘要: |
| 目的探讨微小 RNA-194-5p(miR-194-5p)与泛素连接酶 cullin4A(CUL4A)在肺结核病人中的表达及其意义。方法选取 2016年 12月至 2018年 12月中国平煤神马集团职业病防治院结核病病人为研究对象，确诊为肺结核病人 50例作为肺结核组，潜伏结核感染病人 50例作为潜伏结核感染组，同期该院体检健康者 50例作为对照组。实时荧光定量逆转录聚合酶链反应( qRT-PCR)检测 miR-194-5p、CUL4A mRNA、鸟苷酸结合蛋白 5(GBP5)mRNA的表达；酶联免疫吸附测定( ELISA)检测可溶性 Tim-3(sTim-3)、可溶性 Gal-9(sGal-9)水平；采用 Pearson相关性分析检验肺结核病人 miR-194-5p、CUL4A表达量与 GBP5、 sTim-3、sGal-9的相关性。结核分枝杆菌( Mtb)感染人巨噬细胞系 U937，ELISA检测白细胞介素 -6(IL-6)、肿瘤坏死因子 α(TNF-α)水平。蛋白质印迹法( Western blotting)检测 Wnt/β-连环素( Wnt/β-catenin)信号通路相关蛋白 T细胞因子( TCF)、糖原合成酶激酶 -3(GSK-3β)、 β-catenin表达量。结果与对照组相比，潜伏结核感染组与肺结核组病人血清 miR-194-5p、GBP5 mRNA的表达水平升高( P < 0.05)其中 miR-194-5p:(1.01±0.23)比( 2.32±0.53)比( 3.35±0.20)，sTim-3、sGal-9水平升高( P<0.05)，CUL4A mRNA的表达水平显［(1.03±0.11)比(0.73±0.12)比( 0.41±0.15)，P < 0.05］，潜伏结核感染组与肺结核组各著降低，指标间相比差异有统计学意义( P < 0.05)； miR-194-5p与 CUL4A呈负相关( r=-0.421，P < 0.001)，miR-194-5p与 GBP5、sTim-3、sGal-9呈正相关( P<0.05)； Mtb感染细胞后， IL-6、TNF-α的表达水平升高( P < 0.05)，TCF、GSK-3β、β-catenin表达量升高( P<0.05)。结论 miR-194-5p在肺结核病人中表达上调， CUL4A表达下调，二者可能参与肺结核发生及发展过程。 |
| 英文摘要: |
| Objective To investigate the expression and significance of miR-194-5p and CUL4A in patients with tuberculosis. Methods Patients with tuberculosis from the China Pingmei Shenma Group Occupational Disease Prevention and Treatment Hospitalfrom December 2016 to December 2018 were selected as the research object, and the control group (50 cases), latent combined infec?tion group (50 cases), and pulmonary tuberculosis group (50 cases) were set in the experiment. qRT-PCR was used to detect the expres? sion of miR-194-5p, CUL4A mRNA and GBP5 mRNA. The levels of sTim-3 and sGal-9 were detected by ELISA. Correlation of miR194-5p and CUL4A expression with GBP5, sTim-3, and sGal-9 in patients with tuberculosis was analyzed by Pearson method. Mtb in?fected human macrophage cell line U937 and detected the levels of IL-6 and TNF-α by ELISA. Western blotting was used to detect the expression of TCF, GSK-3β and β-catenin. Results Compared with the control group, the expression levels of serum miR-194-5p and GBP5 mRNA in the latent tuberculosis infection group and the pulmonary tuberculosis group were increased (P<0.05), and miR-1945p: (1.01±0.23) vs. (2.32± 0.53) vs. (3.35±0.20), the levels of sTim-3 and sGal-9 were significantly increased (P < 0.05), and the expres? sion level of CUL4A mRNA was decreased [(1.03±0.11) vs. (0.73±0.12) vs. (0.41±0.15) , P<0.05], there was a statistically significantdifference between the indicators of the latent tuberculosis infection group and the pulmonary tuberculosis group (P < 0.05). miR-1945p was negatively correlated with CUL4A (r=-0.421, P < 0.001), miR-194-5p was positively correlated with GBP5, sTim-3, and sGal-9 (P < 0.05). After Mtb infected cells, the expression levels of IL-6 and TNF-α were increased (P < 0.05), and the expression levels of TCF, GSK-3β and β -catenin were increased (P < 0.05). Conclusion miR-194-5p was up-regulated in tuberculosis patients and CUL4A was down-regulated. Both may be involved in the occurrence and development of tuberculosis. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
