| 陈忠仁,欧宗兴,王蕾,等.Nod样受体蛋白 -3炎性小体在慢性阻塞性肺疾病大鼠气道重塑中的作用[J].安徽医药,2021,25(3):494-496. |
| Nod样受体蛋白 -3炎性小体在慢性阻塞性肺疾病大鼠气道重塑中的作用 |
| Role of NLRP3 inflammasome in airway remodeling in rats with chronic obstructive pulmonary disease |
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| DOI：10.3969/j.issn.1009-6469.2021.03.015 |
| 中文关键词: 肺疾病，慢性阻塞性 Nod信号接头蛋白质类 白细胞介素 -18 白细胞介素 -1 大鼠， Wistar 气管平滑肌 |
| 英文关键词: Pulmonary disease, chronic obstructive Nod signaling adaptor proteins Interleukin-18 Interleukin-1 Rats, Wistar Trachea smooth muscle |
| 基金项目:海南省自然科学基金面上项目( 818MS136) |
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| 中文摘要: |
| 目的探讨 Nod样受体蛋白 -3(NLRP3)炎性小体在慢性阻塞性肺疾病(慢阻肺)大鼠气道重塑中的作用。方法用随机数字表法将 24只 Wistar大鼠均分为慢阻肺组及空白组(各 12只)模型制造成功后测定两组大鼠外周血淋巴细胞 NLRP3 mRNA、肺泡灌洗液炎性因子、气管平滑肌厚度水平。结果慢阻肺组，外周血淋巴细胞 NLRP3 mRNA水平高于空白组( P< |
| 英文摘要: |
| Objective To investigate the role of Nod like receptor protein-3 (NLRP3) inflammasome in airway remodeling in rats with chronic obstructive pulmonary disease (COPD).Methods Using the random number table method,24Wistar rats were divided in?to COPD group and control group (12 rats each).The levels of NLRP3 mRNA in peripheral blood lymphocyte, inflammatory cytokines inbronchoalveolar lavage fluid and airway smooth muscle thickness were measured after the models were successfully established.Re? sults The NLRP3 mRNA level of peripheral blood lymphocytes in COPD group was higher than that in control group (P < 0.05). The thickness of airway smooth muscle in COPD group was (14.25 ± 0.63)μm2/μm, which was higher than that in control group [(10.44 ± 0.61) μm2/μm] (P < 0.05). Compared with the control group, the levels of IL-18 in BALF of COPD Group [(62.98 ± 1.69) vs. (36.24 ± 3.45) ng/L] and IL-1β in BALF of COPD Group [(54.51 ± 1.91) vs. (38.06 ± 0.90)ng/L] were increased (all P < 0.05). The R value be?tween the thickness of tracheal smooth muscle and the relative expression of NLRP3 mRNA in peripheral blood lymphocytes was0.799; the R value between the thickness of tracheal smooth muscle and IL-18 in bronchoalveolar lavage fluid was 0.938; the R value between the thickness of tracheal smooth muscle and IL-1β in bronchoalveolar lavage fluid was 0.970 (all P < 0.05).Conclusion The increased expression of NLRP3 inflammasome in peripheral blood lymphocytes of COPD rats plays a key role in airway remodeling,which provides a new target for the treatment of COPD. |
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