| 唐淑俊,黄涛,张静云,等.血清微小 RNA-34a、沉默信息调节因子 2相关酶 1水平与老年脑梗死病人颈动脉粥样硬化斑块稳定性的关系研究[J].安徽医药,2021,25(3):568-571. |
| 血清微小 RNA-34a、沉默信息调节因子 2相关酶 1水平与老年脑梗死病人颈动脉粥样硬化斑块稳定性的关系研究 |
| Study on the relationships between serum miR-34a and Sirt1 levels and carotid atherosclerotic plaque stability in elderly patients with cerebral infarction |
| |
| DOI：10.3969/j.issn.1009-6469.2021.03.033 |
| 中文关键词: 脑梗死 颈动脉粥样硬化 微小 RNA-34a 沉默信息调节因子 2相关酶 1 老年人 |
| 英文关键词: Cerebralinfarction Carotidatherosclerosis MicroRNA-34a Silenceinformationregulator2-relatedenzymes1 Aged |
| 基金项目: |
|
| 摘要点击次数: 1311 |
| 全文下载次数: 497 |
| 中文摘要: |
| 目的探讨血清微小 RNA-34a(miR-34a)、沉默信息调节因子 2相关酶 1(Sirt1)水平与老年脑梗死病人颈动脉粥样硬化(CAS)斑块稳定性的关系。方法选取 2017年 6月至 2019年 6月邯郸明仁医院收治的老年脑梗死病人 170例作为脑梗死组，同期该院体检健康者 150例作为对照组。均接受颈动脉超声检查，根据检查结果将脑梗死组病人分为无斑块组、稳定斑块组、不稳定斑块组。使用实时荧光定量 PCR(qRT-PCR)检测血清 miR-34a水平，使用酶联免疫吸附测定( ELISA)检测血清 Sirt1水平，使用 AU5800全自动生化分析仪检测三酰甘油、总胆固醇、高密度脂蛋白胆固醇( HDL-C)及低密度脂蛋白胆固醇( LDL-C)，并采用多因素 logistic回归分析影响老年脑梗死病人 CAS不稳定斑块形成的危险因素。结果脑梗死组病人血清 miR-34a(1.88±0.53)水平明显高于对照组( 1.01±0.29)(P < 0.05)血清 Sirt1水平( 0.92±0.26)μg/L明显低于对照组( 2.08±0.57)μg/L(P <0.05)。不稳定斑块组病人年龄明显大于无斑块组( P < 05)；不稳定斑块组、稳定斑块组病人总胆固醇、 LDL-C明显高于无斑.0，块组( P < 0.05)不稳定斑块组病人总胆固醇、 LDL-C明显高于稳定斑块组( P < 0.05)。脑梗死组病人不同 CAS斑块性质血清 miR-34a水平高低，依次为:不稳定斑块组、稳定斑块组、无斑块组，血清 Sirt1水平高低依次为:无斑块组、稳定斑块组、不稳定斑块组，两两比较差异有统计学意义( P < 0.05)。年龄、 miR-34a为老年脑梗死病人 CAS不稳定斑块形成的独立危险因素( P <0.05)，Sirt1为保护性因素(P < 0.05)。结论血清 miR-34a水平升高、 Sirt1水平降低可能与老年脑梗死发生及 CAS不稳定斑块形成有关。 |
| 英文摘要: |
| Objective To investigate the relationship between levels of serum microRNA-34a (miR-34a) and silent information regu? lator 2-related enzyme 1 (Sirt1) and carotid atherosclerosis (CAS) plaque stability in elderly patients with cerebral infarction.Methods 170 elderly patients with cerebral infarction admitted to the Han Dan Mingren Hospital from June 2017 to June 2019 were selected asthe cerebral infarction group, and 150 healthy persons at the same period were selected as the control group. All patients underwent ca?rotid ultrasonography. According to the results, the patients with cerebral infarction were divided into non-plaque group, stable plaque group and unstable plaque group. Serum miR-34a level was detected by real-time fluorescence quantitative PCR (qRT-PCR), serum Sirt1 level was detected by enzyme-linked immunosorbent assay (ELISA), triglyceride (TG), total cholesterol (TC), high density lipopro? tein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were detected by AU5800 automatic biochemical analyzer,and multivariate logistic regression was used to analyze the risk factors of unstable plaque formation in elderly patients with cerebral in?farction.Results The level of serum miR-34a(1.88±0.53) in cerebral infarction group was significantly higher than that in control group(1.01±0.29) (P < 0.05), and the level of serum Sirt1(0.92±0.26)μg/L was significantly lower than that in control group(2.08±0.57) μg/L (P < 0.05). The age of unstable plaque group was significantly older than that of non-plaque group (P < 0.05); TC and LDL-C of unstable plaque group and stable plaque group were significantly higher than those of non-plaque group (P < 0.05); and TC and LDL-C of unstable plaque group were significantly higher than those of stable plaque group (P < 0.05). The levels of serum miR-34a in differ? ent CAS plaque types of cerebral infarction patients were in turn of unstable plaque group, stable plaque group and non-plaque group,the levels of serum Sirt1 were as follows: no plaque group, stable plaque group and unstable plaque group, and there were significantdifferences between the two groups (P < 0.05). Age and miR-34a were independent risk factors for the formation of unstable plaques in CAS in elderly patients with cerebral infarction (P < 0.05), Sirt1 was a protective factor (P < 0.05).Conclusion The increase of serum miR-34a level and the decrease of Sirt1 level may be related to the occurrence of cerebral infarction and the formation of unstableplaques in CAS in the elderly. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
