| 渠茂林.基于 GEO数据库分析非小细胞肺癌癌组织中配对盒基因 8的表达及其与病人预后的关系[J].安徽医药,2021,25(6):1169-1172. |
| 基于 GEO数据库分析非小细胞肺癌癌组织中配对盒基因 8的表达及其与病人预后的关系 |
| Analysis on PAX8 expression in non-small cell lung cancer tissues and its relationship with prognosis based on GEO database |
| |
| DOI：10.3969/j.issn.1009-6469.2021.06.027 |
| 中文关键词: 癌，非小细胞肺 高通量基因表达数据库(GEO数据库) 配对盒基因 8 |
| 英文关键词: Carcinoma, non-small-cell lung Gene Expression Omnibus data base (GEO data base) Paired box gene |
| 基金项目: |
|
| 摘要点击次数: 1677 |
| 全文下载次数: 418 |
| 中文摘要: |
| 目的探讨基于高通量基因表达数据库( Gene expression Omnibus，GEO数据库)分析配对盒基因 8(PAX8)在非小细胞肺癌( NSCLC)病人癌组织中的表达情况及与预后的关系。方法利用在线工具 Oncomine分析 PAX8在 NSCLC与正常肺组织的表达情况，基于 GEO数据库下载相关芯片数据( GSE77803)收集 156例 NSCLC组织的基因表达谱及相关临床数据，采用中位数法将其分为 PAX8低表达组( n=78)和 PAX8高表达组(n=78)，。分析 PAX8的表达与病人性别、年龄、病理类型、 T分期、 N分期和术后复发情况之间的关系；采用生存分析 PAX8的表达与病人预后生存之间的关系；采用基因本体( GO)注释分析 PAX8基因的功能，采用 KEGG PATHWAY分析 PAX8可能参与的信号通路。结果 TCGA Lung 2数据集共检测了 1 537个样本，其中正常肺组织样本 390例，肺癌组织样本共 721例，血液样本 420例，无意义样本 6例。与正常肺组织相比， PAX8在肺腺癌和肺鳞癌中表达水平明显升高( P<0.05)；基于 GEO数据库中 GSE77803(GPL570)数据集分析， NSCLC癌组织中 PAX8的表达水平与病人的 T分期、 N分期和是否复发有关(P<0.05)生存分析显示，癌组织中 PAX8低表达病人的总生存率为 52.94%，中位生存期为 54.6个月，显著高于 PAX8高表达病人( 34.35%，4，8.6个月)(log-rank χ2=4.609，P=0.032)。 PAX8低表达病人无病生存率为 42.50%，中位生存期为 51.7个月，显著高于 PAX8高表达病人( 28.71%，40.8个月)(log-rank χ2=5.712，P=0.017)； GO注释和 KEGG分析显示， PAX8可能参与调节 NSCLC发生发展相关的信号通路的表达。结论 PAX8蛋白在肺腺癌和肺鳞癌中均存在异常高表达， PAX8低表达 NSCLC病人具有更高的生存率。 |
| 英文摘要: |
| Objective To explore expression of paired box gene 8 (PAX8) in cancer tissues of non-small cell lung cancer (NSCLC) patients and its relationship with prognosis based on Gene expression Omnibus (GEO) database.Methods The online tool Oncomine was applied to analyze expression of PAX8 in NSCLC and normal lung tissues. Based on GEO database, related chip data (GSE77803)were downloaded. The gene expression profiles and related clinical data of 156 NSCLC tissues in 156 cases were collected and assigned into PAX8 low-expression group (n=78) and PAX8 high-expression group (n=78) by median method. The relationship betweenexpression of PAX8 and gender, age, pathological type, T stage, N stage, postoperative recurrence was analyzed. The survival analysiswas applied to analyze relationship between PAX8 expression and prognosis survival of patients. Gene Ontology (GO) annotations wereapplied to analyze PAX8 gene function. The signal pathways that PAX8 might participate in were analyzed by Kyoto Encyclopedia ofGenes and Genomes (KEGG) pathway.Results There were 1 537 samples detected by The Cancer Genome Atlas (TCGA) Lung 2 data‐set, including 390 cases with normal lung tissues, 721 cases with lung cancer tissues, 420 cases with blood and 6 cases with meaningless. Compared with normal lung tissues, the level of PAX8 expression was significantly increased in lung adenocarcinoma and lungsquamous carcinoma (P<0.05). Based on GSE77803 (GPL570) dataset analysis in GEO database, the level of PAX8 expression inNSCLC tissues was related with T stage, N stage and recurrence of patients (P<0.05). Survival analysis showed that overall survival rateand median survival time of patients with PAX8 low expression in cancer tissues was significantly higher than those with PAX8 high expression (52.94%, 54.6 months vs. 34.35%, 48.6 months) (log-rank χ2=4.609, P=0.032). The disease-free survival rate and median survival time of patients with PAX8 low expression were significantly higher than those with PAX8 high expression (42.50%, 51.7 months vs. 28.71%,40.8 months) (log-rank χ2=5.712, P=0.017).GO annotations and KEGG analysis showed that PAX8 might be involved in signaling pathways which regulated occurrence and development of NSCLC.Conclusion PAX8 protein is abnormally highly expressed in lung adenocarcinoma and lung squamous carcinoma.The survival rate is higher in NSCLC patients with PAX8 low expression. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
