| 雷雨,刘伟,包黎.首发精神分裂症病人血清微小 RNA-24-2、微小 RNA-30b的检测水平及意义[J].安徽医药,2021,25(6):1204-1207. |
| 首发精神分裂症病人血清微小 RNA-24-2、微小 RNA-30b的检测水平及意义 |
| Detection levels and significance of serum mir-24-2 and mir-30b in patients with first-episode schizophrenia |
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| DOI：10.3969/j.issn.1009-6469.2021.06.035 |
| 中文关键词: 精神分裂症 早期诊断 血清 微小 RNA-24-2 微小 RNA-30b |
| 英文关键词: Schizophrenia Early diagnosis Serum MicroRNA-24-2 MicroRNA-30b |
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| 中文摘要: |
| 目的检测首发精神分裂症( FES)病人血清中微小 RNA-24-2(mir-24-2)、微小 RNA-30b(mir-30b)水平，并探讨血清中 mir-24-2、mir-30b表达水平与 FES发病的关系。方法前瞻性选取 2016年 11月至 2018年 9月于武汉市精神卫生中心就诊的 98例 FES病人为研究对象( FES组)；同期选取 76例健康体检者作为对照(对照组)。分析两组受试者一般资料；采用实时荧光定量逆转录聚合酶链反应( qRT-PCR)检测两组受试者血清中 mir-24-2、mir-30b表达水平，并对 FES病人进行阳性与阴性症状量表( PANSS)评分；分析 FES病人血清中 mir-24-2、mir-30b水平与 PANSS评分相关性；分析血清中 mir-24-2、mir-30b水平对 FES的诊断价值。结果 FES组血清 mir-24-2(1.81±0.58)、 mir-30b水平( 1.46±0.37)、 PANSS总评分( 78.64±12.31)分、阳性症状评分( 12.64±3.73)分、阴性症状评分( 20.56±4.32)分、认知评分( 8.73±1.84)分、兴奋评分( 8.96±2.13)分、抑郁情绪评分( 6.75± 2.06)分显著高于对照组［( 1.00±0.31)、(1.00±0.26)、(35.42±5.37)分、(4.51±1.12)分、(6.94±1.21)分、(3.86±0.95)分、(4.19± 0.38)分、(4.12±1.08)分］(均 P<0.05)； FES病人血清中 mir-24-2、mir-30b水平与 PANSS总评分、阳性症状评分、阴性症状评分、认知评分、兴奋评分、抑郁情绪评分均呈正相关( P<0.05)；血清 mir-24-2、mir-30b诊断 FES的曲线下面积( AUC)分别为 0.863(95%CI:0.808~0.919)、 0.856(95%CI:0.801~0.911)特异度分别为 93.4%、84.2%，灵敏度分别为 68.4%、76.5%；两者联合诊断 FES的 AUC为 0.936(95%CI:0.899~0.973)特异度为8，8.2%，灵敏度为 89.8%。结论血清 mir-24-2、mir-30b水平在 FES病人中明显升高，且与 PANSS评分呈正相关。血清，mir-24-2、mir-30b可能有助于疾病诊断，对评价精神分裂症的早期危险性有重 |
| 英文摘要: |
| Objective To detect the levels of microRNA-24-2 (mir-24-2) and microRNA-30b (mir-30b) in the serum of patients with first-episode schizophrenia (FES), and to explore the relationships between the expressions of mir-24-2 and mir-30b in the serum and the pathogenesis of FES.Methods Ninety-eight FES patients, who were treated in Wuhan Mental Health Center from November 2016to September 2018, were prospectively selected as the study objects (FES group); 76 healthy people were selected as the control groupat the same time. The general data of the two groups were analyzed. The expression levels of mir-24-2 and mir-30b in serum of the two groups were detected by real-time fluorescent quantitative PCR (qRT-PCR), and the positive and negative symptom scale (PANSS)scores of FES patients were evaluated. The correlations between the levels of serum mir-24-2 and mir-30b and PANSS were analyzed; and the diagnostic values of mir-24-2 and mir-30b in serum for FES were analyzed.Results The levels of mir-24-2 [(1.81±0.58)], mir30b [(1.46±0.37)], PANSS total score [(78.64±12.31) points], positive symptom score [(12.64±3.73) points], negative symptom score[(20.56±4.32) points], cognitive score [(8.73±1.84) points], excitement score [(8.96±2.13) points] and depression score [(6.75±2.06)points] in FES group were significantly higher than those in the control group [(1.00±0.31), (1.00±0.26), (35.42±5.37) points, (4.51±1.12) points, (6.94±1.21) points, (3.86±0.95) points, (4.19±0.38) points, (4.12±1.08) points] (all P<0.05). The levels of mir-24-2 and mir-30b in FES patients were positively correlated with PANSS total score, positive symptom score, negative symptom score, cognitivescore, excitement score and depression score (P < 0.05). The area under curve (AUC) of serum mir-24-2 and mir-30b in diagnosing FES were 0.863 (95% CI: 0.808-0.919) and 0.856 (95% CI: 0.801-0.911). The specificity were 93.4% and 84.2%, the sensitivity were68.4% and 76.5%, respectively. The AUC of the combination in diagnosing FES was 0.936 (95% CI: 0.899-0.973), the specificity was 88.2%, and the sensitivity was 89.8%.Conclusions The levels of serum mir-24-2 and mir-30b are significantly increased in patients with FES and positively correlated with PANSS score. Serum mir-24-2 and mir-30b may be helpful for the diagnosis of the disease and |
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