| 朱琳.脑神经胶质瘤替莫唑胺耐药 circRNA-miRNA-mRNA调控网络的构建[J].安徽医药,2021,25(7):1414-1418. |
| 脑神经胶质瘤替莫唑胺耐药 circRNA-miRNA-mRNA调控网络的构建 |
| Construction of circRNA-miRNA-mRNA regulatory network in glioma with temozolomide resistance |
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| DOI：10.3969/j.issn.1009-6469.2021.07.035 |
| 中文关键词: 神经胶质瘤 替莫唑胺 抗药性，肿瘤 circRNA差异表达谱 circRNA-miRNA-mRNA调控网络 |
| 英文关键词: Glioma Temozolomide Drug resistance, neoplasm circRNA differential expression profile circRNA-miRNAmRNA regulatory network |
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| 中文摘要: |
| 目的筛选脑神经胶质瘤替莫唑胺耐药细胞株 U87/TR与亲本细胞株 U87的环状 RNA(circRNA)差异表达谱以及构建关键环状 RNA(circRNA)-微小 RNA(miRNA)-信使 RNA(mRNA)调控网络。方法 2020年 1—6月，应用 circRNAs芯片检测神经胶质瘤替莫唑胺耐药细胞 circRNAs差异表达谱，并进行 RT-PCR验证。用 miRanda数据库预测 circRNA-miRNA靶向结合关系，通过 miRanda v5、TargetScan、miBase数据库预测靶基因， Cytoscape软件构建 circRNA-miRNA-mRNA调控网络。结果 circRNA芯片结果显示，与 U87细胞比较， U87/TR细胞有 313个差异有统计学意义表达的 circRNAs(P<0.05)，其中 145个显著上调， 168个明显下调； RT-qPCR验证结果与芯片结果相符。 miRNA靶位点预测结果显示，关键上调人环状 RNAhsa_circRNA_ 009054，hsa_circRNA_104338(n=3，t=12.09、9.52，P=0.007、0.011)以及下调 hsa_ circRNA_016459，hsa_circRNA_101975(n=3， t=13.86、7.75，P=0.005、0.016)可与 hsa-miR-33a-5p，hsa-miR-15b-3p以及 hsa-miR-193b-5p，hsa-miR-23a-5p等多个 miRNAs靶向结合，关键 circRNA-miRNA-mRNA调控网络包括 4个关键 circRNAs，20个关键 miRNAs，以及 278个关键 mRNAs。结论 circRNA在脑神经胶质瘤替莫唑胺耐药细胞中异常表达， hsa_circRNA_009054，hsa_circRNA_104338以及 hsa_ circRNA_016459， hsa_circRNA_101975等关键 circRNA可能通过 circRNA-miRNA-mRNA调控网络参与脑神经胶质瘤替莫唑胺的耐药过程。 |
| 英文摘要: |
| Objective To screen the differential expression profile of Circular RNA (circRNA) in brain glioma temozolomide-resistant cell line U87/TR and parental cell line U87, and to construct a key Circular RNA (circRNA) -microRNA (miRNA) -messenger RNA(mRNA) regulatory network.Methods From January to June 2020, circRNAs chip was used to detect the differential expression profile of circRNAs in glioma-resistant temozolomide cells and verified by RT-PCR. miRanda database was used to predict the circRNA-miRNA target binding relationship. miRanda v5, TargetScan, and miBase databases were used to predict the target genes. Cytoscape software was used to construct the circRNA-miRNA-mRNA regulatory network.Results The results of circRNA microarrayshowed that U87/TR cells had 313 significantly differentially expressed circRNAs compared with U87 cells (P<0.05), of which 145 were significantly up-regulated and 168 were significantly down-regulated, RT-PCR verification results were consistent with the results of the chip. The prediction results of miRNA target sites showed that the key up-regulation of hsa_circRNA_009054, hsa_circRNA_ 104338 (n=3, t=12.09, 9.52, P=0.007, 0.011) and down-regulation of hsa_circRNA_016459, hsa_circRNA_101975 (n=3, t=13.86, 7.75, P=0.005, 0.016) can target multiple miRNAs such as hsa-miR-33a-5p, hsa-miR-15b-3phsa-miR-193b-5p and hsa-miR-23a-5p, the key circRNA-miRNA-mRNA regulatory network included 4 key circRNAs, 20 key miRNAs, and 278 key mRNAs.Conclusion circRNA is abnormally expressed in temozolomide-resistant cells of cerebral glioma. Key circRNAs such as hsa_circRNA_009054,hsa_circRNA_104338 and hsa_circRNA_016459, hsa_circRNA_101975 and other key circRNAs may participate in the drug resistance process of temozolomide in brain glioma through the circRNA-miRNA-mRNA regulatory network. |
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