| 杨隆良,马瑜,郭虎林.lncRNA LINC00858靶向微小 RNA-363-3p促进肝癌细胞增殖、迁移和侵袭[J].安徽医药,2021,25(8):1601-1604. |
| lncRNA LINC00858靶向微小 RNA-363-3p促进肝癌细胞增殖、迁移和侵袭 |
| lncRNA LINC00858 promotes cell proliferation, migration and invasion in liver cancer by targeting miR-363-3p |
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| DOI：10.3969/j.issn.1009-6469.2021.08.030 |
| 中文关键词: 肝肿瘤 lncRNA LINC00858 微小 RNA-363-3p 增殖 迁移 侵袭 |
| 英文关键词: Liver neoplasms LncRNA LINC00858 miR-363-3p Proliferation Migration Invasion |
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| 中文摘要: |
| 目的探讨 LINC00858通过靶向 miR-363-3p对肝癌 HCCLM3细胞生物学行为的影响。方法选取 2017年 1月至 2019年 1月青海省第五人民医院收治的肝癌病人 30例，获取其肝癌组织及其癌旁组织。设置 si-NC组、 si-LINC00858组、 siLINC00858+anti-miR-NC组和 si-LINC00858 +anti-miR-363-3p组。实时荧光定量聚合酶链式反应( RT-qPCR)检测 LINC00858和 miR-363-3p表达水平；双荧光素酶报告实验验证 LINC00858和 miR-363-3p的靶向关系； CCK-8检测细胞增殖活性； Transwell检测细胞迁移和侵袭；蛋白质印迹法( Western blotting)法检测相关蛋白表达。结果肝癌组织中 LINC00858高表达( 2.85±0.27)，miR-363-3p低表达( 0.58±0.05)。 LINC00858靶向调控 miR-363-3p；抑制 LINC00858能够上调 p21表达，下调细胞周期蛋白 D1(CyclinD1)、基质金属蛋白酶 2(MMP-2)、基质金属蛋白酶 9(MMP-9)表达，降低细胞活力、迁移数、侵袭数( P<0.05)。干扰 miR-363-3p表达逆转了抑制 LINC00858表达对肝癌 HCCLM3细胞增殖、迁移和侵袭的影响。结论抑制 LINC00858通过靶向上调 miR-363-3p抑制肝癌细胞的增殖、迁移和侵袭。 |
| 英文摘要: |
| Objective To investigate the effects of LINC00858 on the biological behaviour of liver cancer HCCLM3 cells by targeting miR-363-3p.Methods si-NC group, si-LINC00858 group, si-LINC00858+anti-miR-NC group and si-LINC00858+anti-miR-3633p group were set. RT-qPCR to detect the expressions of LINC00858 and miR-363-3p;dual luciferase report assay verified the targeting relationship between LINC00858 and miR-363-3p;Cell proliferation activity was test using CCK-8 methods; cell migration and invasionwas evaluated using Transwell; and related proteins expression was analyzed using Western blot.Results In liver cancer tissues, LINC00858(2.85±0.27) is highly expressed and miR-363-3p(0.58±0.05) is lowly expressed. LINC00858 targeted regulation of miR363-3p; LINC00858 inhibition could up-regulate p21 expression, down-regulate cyclinD1, MMP-2 and MMP-9 expression, and reduce the cell viability, migration number and invasion number (P<0.05). Interfering with the expression of miR-363-3p reversed the inhibitory effect of LINC00858 inhibition on HCCLM3 cells proliferation, migration and invasion.Conclusion Inhibition of LINC00858 inhibits the proliferation, migration and invasion of liver cancer cells by targeting up-regulation of miR-363-3p. |
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