| 吴佳佳,吴戈,胡海圣,等.变应性支气管肺曲霉病与过敏性哮喘病人血清免疫因子差异性的初步探讨[J].安徽医药,2021,25(9):1788-1792. |
| 变应性支气管肺曲霉病与过敏性哮喘病人血清免疫因子差异性的初步探讨 |
| Preliminary study on the differences of serum immune factors between patients with allergic bronchopulmonary aspergillosis and allergic asthma |
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| DOI：10.3969/j.issn.1009-6469.2021.09.021 |
| 中文关键词: 曲霉菌病，变应性支气管肺 过敏性哮喘 白细胞介素 -5 白细胞介素 -8 |
| 英文关键词: Aspergillosis, allergic bronchopulmonary Allergic asthma IL-5 IL-8 |
| 基金项目:国家自然科学基金项目( 81572063)；广医大高水平大学建设科研教学学术提升项目( B185004118) |
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| 中文摘要: |
| 目的探索变应性支气管肺曲霉病( ABPA)与过敏性哮喘病人之间血清中细胞因子表达水平的差异，并检测有差异的细胞因子是否能作为辅助诊断 ABPA的生物标志物。方法筛选 2015年 1月至 2016年 6月广州医科大学附属第一医院的 ABPA病人 23例和过敏性支气管哮喘病人 24例，病人血清中的胸腺基质淋巴细胞生成素( TSLP)和胸腺活化调节趋化因子(TARC)通过酶联免疫吸附测定( ELISA)检测，总免疫球蛋白 E(IgE)和烟曲霉特异性 IgE通过 ImmunoCAP检测，细胞因子 γ干扰素、白细胞介素( IL)-5、IL-8、IL-10、IL-13和 IL-17A通过超敏多因子电化学发光免疫分析仪( MSD)检测。结果 ABPA与哮喘病人之间血清中 TARC以及 TSLP水平差异无统计学意义( P > 0.05)。但 ABPA病人血清中 IL-5［( 2.02±1.43)ng/L比( 0.47±0.39)ng/L，P < 0.01］以及 IL-8［(73.31±59.00)ng/L比( 36.87±41.95)ng/L，P < 0.05］水平均显著高于过敏性哮喘病人。此外，组间血清中 γ干扰素、 IL-10、IL-13和 IL-17A细胞因子差异无统计学意义( P > 0.05)。在受试者工作特征曲线( ROC)分析中，两IL-5和 IL-8鉴别诊断 ABPA与哮喘的最佳截断点分别为 0.89 ng/L和 16.41 ng/L，此时的 ROC曲线下面面积分别为 0.902和0.720。结论与哮喘病人相比， ABPA病人血清中 IL-5和 IL-8水平显著升高；血清中 IL-5和 IL-8细胞因子水平或可作为辅助诊断 ABPA的指标。 |
| 英文摘要: |
| Objective To investigate the differences of levels of multiple cytokines between patients with allergic bronchopulmonaryaspergillosis (ABPA) and allergic asthma, and to explore whether the cytokines could be indicators of differential diagnosis of ABPA.Methods A total of 23 patients with ABPA and 24 with allergic asthma in the First Hospital Affiliated to Guangzhou Medical University From January 2015 to June 2016 were enrolled. Enzyme linked immunosorbent assay (ELISA) was used to detected thymic stromallymphopoietin (TSLP) and thymus-and activation-regulated chemokine (TARC). Total IgE and Aspergillus fumigatus specific IgE were detected by ImmunoCAP, while cytokines (IFN-γ, IL-5, IL-8, IL-10, IL-13 and IL-17A) were detected by Meso Scale Discovery (MSD). Results No significant difference was found in serum levels of either TARC or TSLP between patients with ABPA and asthma (P > 0.05). Among the detected cytokines, serum levels of both IL-5 and IL-8 were significantly higher in patients with ABPA comparing with asthma [IL-5: (2.02±1.43) ng/L vs. (0.47±0.39) ng/L, P < 0.01; IL-8: (73.31±59.00) ng/L vs. (36.87±41.95) ng/L, P < 0.05]. There was no statistical difference in the comparison of other cytokines (IFN-γ, IL-10, IL-13 and IL-17A) between the two groups (P > 0.05). In ROC analysis, the optimal cut-off value for IL-5 and IL-8 in differential diagnosis of ABPA and asthma was 0.89 ng/L and 16.41 ng/ L, respectively, and the area under the curve (AUC) at the cut-off values were 0.902 and 0.720, respectively.Conclusions Compared with asthma, patients with ABPA have significantly higher serum levels of IL-5 and IL-8. Additionally, both cytokines could be promising indicators in the diagnosis of ABPA. |
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