| 孔凡鑫,王恒珍,周俊梅.高效液相色谱法检测尿毒清颗粒(无糖型)中芍药苷、大车前苷和丹参酮 ⅡA成分的含量[J].安徽医药,2021,25(11):2178-2181. |
| 高效液相色谱法检测尿毒清颗粒(无糖型)中芍药苷、大车前苷和丹参酮 ⅡA成分的含量 |
| HPLC method for determination contents of paeoniflorin, plantamoside and tanshinone ⅡA in Niaoduqing granules (sugar-free type) |
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| DOI：10.3969/j.issn.1009-6469.2021.11.013 |
| 中文关键词: 尿毒症 色谱法，高压液相 尿毒清颗粒 芍药苷 大车前苷 丹参酮 Ⅱ |
| 英文关键词: Uremia Chromatography,high pressure liquid(HPLC) Niaoduqing granules Paeoniflorin Plantamoside Tanshinone ⅡA |
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| 中文摘要: |
| 目的建立检测尿毒清颗粒(无糖型)中芍药苷、大车前苷和丹参酮 ⅡA成分含量的方法。方法取尿毒清颗粒(无糖型)适量，研细，取约 1.0 g，精密称定，置 50 mL棕色容量瓶中，加 90%甲醇溶液超声处理 30 min后，放冷定容， 0.45 μm滤膜滤过， Waters Spherisorb ODS1液相色谱柱分离。检测波长为芍药苷( 230 nm)、大车前苷( 330 nm)、丹参酮 ⅡA(270 nm)；流动相为乙腈( A)-0.2%磷酸溶液( B)，梯度洗脱；流速为 1.0 mL/min，柱温为 25 ℃，进样量为 10 μL，二极管阵列检测器定量检测。结果芍药苷、大车前苷和丹参酮 ⅡA线性范围分别为 1.427~57.06 μg/mL、2.255~90.2 μg/mL和 0.498~19.9 μg/mL(R2为 0.992~0.996)；平均加样回收率分别为 99.39%，97.99%和 98.41%；重复性 RSD分别为 3.16%、3.38%和 2.69%；样品稳定性良好。结论 HPLC法重复性好、操作简单，弥补了单一指标成分含量测定作为质控手段的不足，为提高该产品的质量控制提供技术依据。 |
| 英文摘要: |
| Objective To explore the method for determination contents of paeoniflorin, plantamoside and tanshinone ⅡA in Niaoduq? ing Granules (sugar-free type).Methods Appropriate amount of Niaoduqing granules (sugar-free type) was taken and grinded. 1.0 g samples was weighed up precisely to 50 mL brown glass flask volumetric, 90% methanol solution was added to the flask and ultrasound for 30minutes. Cooling the samples and fixed to constant volume. After filtered by 0.45 μm membrane filter, samples was seperated on WatersSpherisorb ODS1 columm. Detection wavelength were as follows: paeoniflorin (230 nm), plantamoside (330 nm) and tanshinone ⅡA (270 nm). Mobile phase was acetonitrile (A)-0.2% phosphoric acid solution (B).Gradient elution, flow rate: 1.0 mL/min, column temperature:25 ℃and injection volumewas 10 μL. Diode array detector was for quantitative detection.Results Paeoniflorin, plantamoside and tanshinone ⅡAshowed good linear relationships in following concentration range 1.427-57.06 μg/mL, 2.255-90.2 μg/mL and 0.498-19.9 μg/ mL, respectively.With correlation coefficients R2all among 0.992-0.996. Average recoveries were 99.39%, 97.99% and 98.41%, respectively. Relative standard deviation (RSD) of repeatability (n=6) was 3.16%, 3.38% and 2.69%, respectively. Stability of samples was good. Conclusion The HPLC method has good repeatability and simple operation, which makes up for the deficiency of single index component content determination as a quality control method, and provides a technical basis for improving the quality control of the product. |
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