| 蒙毅,吴月霞,龚纯,等.基于网络药理学研究半夏治疗功能性消化不良的作用机制[J].安徽医药,2022,26(1):16-20. |
| 基于网络药理学研究半夏治疗功能性消化不良的作用机制 |
| Study on the mechanism of Pinellia ternate in the treatment of functional dyspepsia based on network pharmacology |
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| DOI：10.3969/j.issn.1009-6469.2022.01.004 |
| 中文关键词: 半夏属 消化不良 基因， bcl-2 半胱氨酸天冬氨酸蛋白酶 细胞凋亡 网络药理学 靶点 信号通路 京都基因和基因组百科全书(KEGG) |
| 英文关键词: Pinellia Dyspepsia Genes,bcl-2 Caspases Apoptosis Network pharmacology Target Signal pathway Kyoto encyclopedia of genes and genomes |
| 基金项目:广西自然科学基金项目( 2018GXNSFAA281063) |
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| 中文摘要: |
| 目的探讨半夏治疗功能性消化不良的作用机制。方法本研究起止 2020年 1—2月。通过 TCMSP数据库检索得到半夏的活性成分和作用靶点，同时借助 UniProt、HCBI和 PubMed等数据库，得到药物活性成分靶点对应的基因名。利用 Gene Cards和 OMIM数据库得到功能性消化不良的相关治疗靶点，将其与半夏活性成分靶点取交集后得到药效靶点。将交集靶点通过 STRING 11.0数据库进行蛋白质 -蛋白质的相互作用网络分析得到潜在的治疗核心蛋白。最后采用 KOBAS 3.0平台和 DAVID 6.8数据库进行 GO功能富集分析和 KEGG信号通路富集分析，研究半夏治疗功能性消化不良靶点的作用机制。结果筛选得出半夏的 13个活性成分和 74个活性成分靶点基因以及 35个交集基因。通过蛋白质 -蛋白质相互作用网络分析，半夏治疗功能性消化不良可能与 B淋巴细胞瘤 -2基因( Bcl-2)、 RACα丝氨酸 /苏氨酸蛋白激酶( AKT1)、半胱氨酸蛋白酶 -3(CASP3)、半胱氨酸蛋白酶 -9(CASP9)等靶点蛋白相关。 GO功能富集分析得到 66个 GO条目( P<0.05)，通过分析我们可以知道半夏治疗功能性消化不良主要与儿茶酚胺结合、内肽酶活性、核受体活性、转录因子活性、凋亡过程中的半胱氨酸型内肽酶活性等功能相关。 KEGG信号通路分析得到 97条信号通路( P<0.05)通过筛选主要与凋亡 -多物种、凋亡 -乙型肝炎、大肠癌、肿瘤坏死因子等信号通路相关，我们发现这些通路中均含有 B淋巴，细胞瘤 -2基因( Bcl-2)、半胱氨酸蛋白酶 |
| 英文摘要: |
| Objective To explore the mechanism of Pinellia ternate in the treatment of functional dyspepsia.Method This studystarted and ended from January to February 2020, the active components and action targets of Pinellia ternata were retrieved from TC-MSP database. At the same time, the gene names corresponding to the drug active component targets were obtained with the help of Uni.Prot, hcbi and PubMed databases. Gene cards and OMIM database were used to obtain the relevant therapeutic targets of functionaldyspepsia, and the pharmacodynamic targets were obtained after intersection with the active component targets of Pinellia ternata. Theintersection targets were analyzed by protein-protein interaction network through STRING 11.0 database to obtain potential therapeuticcore proteins. Finally, KOBAS 3.0 platform and DAVID 6.8 database were used for GO function enrichment analysis and KEGG signalpathway enrichment analysis to study the mechanism of Pinellia ternata in the treatment of functional dyspepsia.Results 13 active components, 74 target genes and 35 cross genes of Pinellia ternata were screened . The analysis of protein protein interaction networkfound that Pinellia ternata treatment of functional dyspepsia might be related to Bcl-2, AKT1, CASP3, CASP9, Jun and other target pro-teins. 66 GO items were obtained by GO functional enrichment analysis (P<0.05). The analysis results found that Pinellia treatment forfunctional dyspepsia was mainly related to catecholamine binding, endopeptidase activity, nuclear receptor activity, transcription factoractivity, cysteine endopeptidase activity in the process of apoptosis and other functions. KEGG signal pathway analysis showed 97 sig-nal pathways (P<0.05), which were mainly related to apoptosis -multiple fields, apoptosis, hepatitis B, colorative cancer, TNF signalingpathway and other signal pathways, and these pathways all contained Bcl-2, caspase, Bax and other apoptosis factors.Conclusion Pi-nellia ternate can play a role in the treatment of functional dyspepsia through multi-component, multi-target and multi-channel. Amongthem, the inhibition or enhancement of its factor expression in the apoptotic pathway, and ultimately the inhibition of apoptosis of tissuecells will be one of the important mechanisms of Pinellia ternate in the treatment of functional dyspepsia. |
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