| Objective To investigate whether parthenolide can affect the proliferation and apoptosis of uveal melanoma cells by reg. ulating NF-κB signaling pathway.Methods The uveal melanoma cells MUM2B were treated with parthenolide at a final concentrationof 0, 5, 10, 20 μg/mL; MUM2B cells were treated with PDTC, PMA, and recorded as PDTC group, PMA groups, MUM2B cells withoutany treatment were used as control group; 10 μg/mL of parthenolide and PMA acted on MUM2B cells, which were recorded as 10 μg/mL+PMA group. Cell proliferation was detected by MTT assay; apoptosis was detected by flow cytometry; protein expression was detect.ed by Western Blotting.Results The apoptosis rates of melanoma cell line MUM2B with 0, 5, 10, 20 μg/mL were (5.54±0.56)%,(13.19±1.65)%, (28.49±3.90)%, and (49.36±4.27)%, respectively, the expression of Bcl-2 related X (Bax) was (0.21±0.03), (0.36±0.05), (0.58±0.07), and (0.85±0.08), respectively, and the expression of B-cell lymphoma-2 (Bcl-2) protein was (0.91±0.09), (0.53±0.07),(0.35±0.04) and (0.20±0.03), respectively. Compared with the parthenolide 0 μg/mL group, the different concentrations of parthenolideinhibited the proliferation of melanoma cells MUM2B, promoted the apoptosis of MUM2B, inhibited the expression of Bcl-2 protein, promoted the expression of Bax protein, and inhibited the activation of NF-κB signaling pathway. Inhibition of NF-κB signaling path. way could inhibit the proliferation of uveal melanoma cell line MUM2B and promote apoptosis. Activation of NF-κB signaling pathway could reverse the proliferation inhibition and apoptosis-promoting effect of parthenolide on uveal melanoma cell line MUM2B.Conclu. sion Parthenolide can inhibit the proliferation of uveal melanoma cell line MUM2B and promote its apoptosis. The mechanism may berelated to NF-κB signaling pathway, which will provide new ideas and targets for the treatment of melanoma. |
