| 张承中,尹占海,李存宽,等.氟伐他汀调控 Sirt3对过氧化氢诱导的骨髓间充质干细胞增殖凋亡的影响[J].安徽医药,2022,26(2):239. |
| 氟伐他汀调控 Sirt3对过氧化氢诱导的骨髓间充质干细胞增殖凋亡的影响 |
| Effect of fluvastatin on proliferation and apoptosis of bone marrow mesenchymal stem cells induced by hydrogen peroxide |
| |
| DOI：10.3969/j.issn.1009-6469.2022.02.007 |
| 中文关键词: 间质干细胞 氟伐他汀 Sirt3 过氧化氢 骨髓间充质干细胞 增殖 凋亡 |
| 英文关键词: Mesenchymal stem cells Fluvastatin Sirt3 Hydrogen peroxide Bone marrow mesenchymal stem cells Prolif. eration Apoptosis |
| 基金项目: |
|
| 摘要点击次数: 1916 |
| 全文下载次数: 748 |
| 中文摘要: |
| 目的探讨氟伐他汀对过氧化氢诱导的骨髓间充质干细胞( BMSCs)增殖凋亡的影响及作用机制。方法用 400 μmol/L的过氧化氢处理 BMSCs作为模型组； BMSCs用 400 μmol/L的过氧化氢培养的同时分别加入 0.01、0.1、1 μmol/L氟伐他汀作为干预组；未添加任何物质正常培养的细胞作为正常组；将 si-NC、si-Sirt3转染至 BMSCs中再用 400 μmol/L的过氧化氢、 0.1 μmol/L氟伐他汀处理作为干预组 2+si-NC组、干预组 2+si-Sirt3组。蛋白质印迹( Western Blotting)法检测 Sirt3蛋白表达水平；四甲基偶氮唑盐比色法( MTT)检测细胞存活率；流式细胞术检测细胞凋亡。结果与正常组相比，模型组 BMSCs细胞存活率降低［( 41.57±4.32)%比( 100.40±10.23)%］，细胞凋亡率升高［( 38.57±4.04)%比( 8.28±0.92)%］，Sirt3表达水平降低［( 0.31± 0.03)比( 1.01±0.10)］均差异有统计学意义(P<0.05)；与模型组相比，氟伐他汀干预组细胞存活率明显升高细胞凋亡率降低， Sirt3表达水平升高，异有统计学意义( P<0.05)。提示抑制 Sirt3能逆转氟伐他汀对过氧化氢诱导的 BMSCs细胞增殖抑制均差，和凋亡促进作用。结论氟伐他汀能抑制过氧化氢诱导的 BMSCs细胞凋亡，其机制可能与上调 Sirt3有关。 |
| 英文摘要: |
| Objective To investigate the effect and mechanism of fluvastatin on proliferation and apoptosis of bone marrow mesen.chymal stem cells (BMSCs) induced by hydrogen peroxide.Methods BMSCs were treated with 400 μmol/L hydrogen peroxide as amodel group; BMSCs were cultured with 400 μmol/L hydrogen peroxide while adding 0.01, 0.1,1 μmol/L fluvastatin as interventiongroup; normal cultured cells without any additives served as normal group; BMSCs transfecte with si-NC and si-Sirt3 and treated with 400 μmol/L hydrogen peroxide and 0.1 μmol/L fluvastatin were designated as intervention group 2+si-NC group, intervention group 2+ si-Sirt3 group. The expression of Sirt3 protein was detected by Western Blot; cell viability was detected by MTT assay; cell apoptosiswas detected by flow cytometry.Results Compared with normal group, the cell survival rate of BMSCs in model group was significant. ly decreased [(41.57±4.32) % vs. (100.40±10.23) %], the apoptosis rate was significantly increased [(38.57±4.04) % vs. (8.28±0.92) %], and the expression of Sirt3 was significantly decreased [(0.31±0.03) vs. (1.01±0.10) ] (P<0.05); Compared with model group, the cellsurvival rate of the intervention group was significantly increased, and the apoptosis rate was significantly decreased. The expression ofSirt3 was significantly increased (P<0.05). Inhibition of Sirt3 reversed the effect of fluvastatin on proliferation inhibition and apoptosisof BMSCs induced by hydrogen peroxide.Conclusion Fluvastatin can inhibit the apoptosis of BMSCs induced by hydrogen peroxide, and its mechanism may be related to up-regulation of Sirt3. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
