文章摘要
张霓,伍文才,李春燕,等.微小 RNA-588靶向调控脾酪氨酸激酶的表达及其对胃癌细胞增殖、凋亡的作用[J].安徽医药,2022,26(2):378-382.
微小 RNA-588靶向调控脾酪氨酸激酶的表达及其对胃癌细胞增殖、凋亡的作用
Mir-588 targeted regulation of spleen tyrosine kinase expression and its effect on proliferation and apoptosis of gastric cancer cells
  
DOI:10.3969/j.issn.1009-6469.2022.02.040
中文关键词: 胃肿瘤  微小 RNA-588  脾酪氨酸激酶  增殖  凋亡
英文关键词: Stomach neoplasms  MiR-588  Syk  Proliferation  Apoptosis
基金项目:
作者单位
张霓 重庆市九龙坡区第二人民医院消化内科重庆 400052 
伍文才 重庆市九龙坡区第二人民医院消化内科重庆 400052 
李春燕 重庆市九龙坡区第二人民医院消化内科重庆 400052 
陈欣 重庆市九龙坡区第二人民医院消化内科重庆 400052 
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中文摘要:
      目的探讨微小 RNA-588(miR-588)对胃癌细胞增殖、凋亡的影响以及潜在的作用机制。方法运用实时荧光定量逆转录聚合酶链式反应( qRT-PCR)检测胃癌细胞 SGC-7901、BGC-823和正常胃黏膜细胞 GES-1中 miR-588的表达水平;将胃癌细胞 SGC-7901、BGC-823分为 anti-miR-NC组、 anti-miR-588组、 anti-miR-588+si-NC组、 anti-miR-588+si-脾酪氨酸激酶( Syk)组;蛋白质印迹法( Western blotting)检测蛋白表达;四甲基偶氮唑盐微量酶反应比色( MTT)法检测 BGC-823细胞活性;流式细胞术检测 BGC-823细胞凋亡率;双荧光素酶报告实验检测 miR-588和 Syk的靶向关系。结果胃癌细胞 SGC-7901、BGC-823中 miR-588的表达水平高于 GES-1细胞[(0.58±0.06)、(0.84±0.08)比( 0.36±0.04)P < 0.05]。抑制 miR-588表达可抑制胃癌细胞增殖,诱导细胞凋亡;促进 P21、Bax蛋白的表达,抑制 cyclin D1、Bcl-2蛋白的表达,。miR-588靶向负调控 Syk的表达,抑制 Syk表达能逆转抑制 miR-588对细胞 BGC-823细胞的作用。结论 miR-588可抑制胃癌细胞细胞增殖,诱导细胞凋亡,其机制可能与 Syk有关。
英文摘要:
      Objective To investigate the effect of miR-588 on proliferation and apoptosis of gastric cancer cells and its potential mechanism.Methods qRT-PCR was used to detect the expression level of miR-588 in gastric cancer cells SGC-7901, BGC-823 and normal gastric mucosal cells GES-1; gastric cancer cells SGC-7901 and BGC-823 were divided into anti-miR-NC group, anti-miR-588 group, anti-miR-588+si-NC group, anti-miR-588+si-Syk group; Western Blot was used to detect protein expression; MTT method was employed to detect BGC-823 cell viability; flow cytometry was performed to detect BGC-823 cell apoptosis rate; the dual luciferase re. porter experiment was used to detect the targeting relationship between miR-588 and Syk.Results The expression level of miR-588 in gastric cancer cells SGC-7901 and BGC-823 was higher than that of GES-1 cells [(0.58±0.06),(0.84±0.08)vs.(0.36±0.04),P < 0.05]. In. hibition of miR-588 expression could inhibit the proliferation of gastric cancer cells, induce apoptosis, promote the expression of P21and Bax proteins, and inhibit the expression of cyclin D1 and Bcl-2 proteins. miR-588 targeting negatively regulated the expression of Syk, and inhibition of Syk expression could reverse the effect of inhibiting miR-588 on BGC-823 cells.Conclusion MiR-588 can in. hibit cell proliferation and induce apoptosis of gastric cancer cells, and its mechanism may be related to Syk.
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