| 赵娜,殷莉,秦晓楠.基于白细胞介素 13/信号传导和转录激活因子 6信号通路研究甘草酸二铵对慢性阻塞性肺疾病大鼠气管黏液高分泌的影响[J].安徽医药,2022,26(7):1406-1410. |
| 基于白细胞介素 13/信号传导和转录激活因子 6信号通路研究甘草酸二铵对慢性阻塞性肺疾病大鼠气管黏液高分泌的影响 |
| Effects of diammonium glycyrrhizinate on tracheal mucus hypersecretion in rats with COPD based on the IL-13/STAT6 signaling pathway |
| |
| DOI：10.3969/j.issn.1009-6469.2022.07.031 |
| 中文关键词: 肺疾病，慢性阻塞性 甘草酸二铵 白细胞介素 13 信号转导与转录激活因子 6 气管黏液 大鼠， Sprague-dawley |
| 英文关键词: Pulmonary disease,chronic obstructive Diammonium glycyrrhizinate Interleukin-13 Signal transducer and activa-tor of transcription 6 Tracheal mucus Rats,Sprague-Dawley |
| 基金项目: |
|
| 摘要点击次数: 854 |
| 全文下载次数: 333 |
| 中文摘要: |
| 目的基于白细胞介素 13(IL-13)/信号传导和转录激活因子 6(STAT6)信号通路观察甘草酸二铵盐对慢性阻塞性肺疾病( COPD)大鼠气管黏液高分泌的影响。方法 2019年 6月至 2020年 2月，从珠海百试通生物科技有限公司购买健康雄性 SD大鼠。将 SD雄性大鼠按随机数字表法分为正常组、模型组、盐酸氨溴索组和甘草酸二铵低、中、高剂量组，每组 15只。采用香烟烟熏联合脂多糖( LPS)建立 COPD大鼠模型，除正常组和模型组注射生理盐水外，其余各组均于尾部静脉注射相应剂量的药物，每天 1次，持续 14 d。通过检测各组大鼠的肺阻力(RI)、肺顺应性( Cdyn)以及酚红排痰实验反映大鼠肺功能变化情况； ELI-SA检测肺泡灌洗液中 IL-13的水平变化； HE染色反映肺组织的病理学变化；实时定量荧光 PCR(qRT-PCR)和 Western blotting检测各组肺组织中 IL-13、STAT6和黏蛋白 5AC(MUC5AC)mRNA及蛋白表达水平。结果与正常组相比，模型组大鼠 Cdyn降低， RI［( 2.39±0.30)cmH2O·mL-1·s-1比( 0.85±0.11)cmH2O·mL-1·s-1］、排痰量［( 1.09±0.14)mg/L比( 0.55±0.07)mg/L］、肺泡灌洗液中 IL-13［(34.28±5.88)ng/L比( 10.36±1.67)ng/L］水平显著升高( P<0.05)，肺组织出现病理学损伤， p-STAT6/STAT6［(1.33±0.23)比( 0.72±0.14)］水平以及 IL-13蛋白［(0.47±0.07)比( 0.17±0.03)］和 MUC5AC mRNA及蛋白［(0.78±0.15)比( 0.15±0.04)］水平均有升高( P<0.05)；与模型组相比，甘草酸二铵低、中、高剂量组和盐酸氨溴索组 Cdyn增加， RI、排痰量、肺泡灌洗液中 IL-13水平显著下降( P<0.05)，肺组织损伤程度降低， p-STAT6/STAT6以及 IL-13蛋白和 MUC5AC mRNA及蛋白水平均有降低( P<0.05)；其中甘草酸二铵组作用效果最为明显。结论甘草酸二铵能抑制 COPD模型大鼠 IL-13/STAT6信号通路，并可能藉此减轻气管黏液高分泌，改善气管阻塞。 |
| 英文摘要: |
| Objective To observe the effects of diammonium glycyrrhizinate on tracheal mucus hypersecretion in rats with chronicobstructive pulmonary disease (COPD) based on the interleukin-13 (IL-13)/signal transducer and activator of transcription 6 (STAT6) signaling pathway.Methods From June 2019 to February 2020, healthy male Sprague-Dawley (SD) rats were purchased from Zhuhai BesTest Bio-Tech Co., Ltd. The rats were divided into the normal group, model group, ambroxol hydrochloride group and diammoniumglycyrrhizinate low-dose, medium-dose and high-dose groups according to the random number table method, with 15 rats in each group.The COPD rat model was established by cigarette smoking combined with lipopolysaccharide (LPS). Except for the normal group andthe model group, which were injected with normal saline, the other groups were injected with the corresponding dose of drugs throughthe tail vein once a day for 14 days. The pulmonary resistance (RI), lung compliance (Cdyn) and phenol red sputum excretion test of ratsin each group were detected to reflect the changes in pulmonary function. The level of IL-13 in bronchoalveolar lavage fluid (BALF) was detected by enzyme-linked immunosorbent assay (ELISA). The pathological changes in the lung tissue were reflected by HE stain-ing. Quantitative real-time PCR (qRT-PCR) and Western blotting were used to detect the mRNA and protein expression levels of IL-13, STAT6 and mucin 5ac (MUC5AC).Results Compared with the normal group, the rats in the model group had lower Cdyn, RI [(2.39±10.30) cmH2O·mL-1·vs. (0.85±0.11) cmH2O·mL-1·s-1], sputum volume [(1.09±0.14) mg/L vs. (0.55±0.07) mg/L] and IL-13 [(34.28± 5.88) ng/L vs. (10.36±1.67) ng/L] levels in BALF were significantly increased (P < 0.05); lung tissue showed pathological damage, p-STAT6/STAT6 [(1.33±0.23) vs. (0.72±0.14)] level and IL-13 protein [(0.47±0.07) vs. (0.17±0.03)] and MUC5AC mRNA and protein [(0.78±0.15) vs. (0.15±0.04)] levels were increased (P < 0.05). Compared with the model group, Cdyn increased, and RI, sputum vol-ume, and IL-13 levels in bronchoalveolar lavage fluid decreased significantly in the low-, medium-and high-dose groups of diammoni-um glycyrrhizinate and ambroxol hydrochloride group (P < 0.05). The degree of lung tissue injury was reduced, and the levels of p-STAT6/STAT6, IL-13 protein and MUC5AC mRNA and protein were decreased (P < 0.05), and the effect of diammonium glycyrrhiz-inate group was the most obvious.Conclusions Diammonium glycyrrhizinate can inhibit the IL-13/STAT6 signaling pathway in COPD model rats, and may reduce tracheal mucus hypersecretion and improve tracheal obstruction. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
