| 王敏,高湘玲.微小 RNA-92a靶向 Kru.ppel样因子 2基因促进卵巢癌细胞侵袭的作用[J].安徽医药,2022,26(7):1411-1414. |
| 微小 RNA-92a靶向 Kru.ppel样因子 2基因促进卵巢癌细胞侵袭的作用 |
| The role of microRNA-92a targeting the Krüppel-like factor 2 gene in promoting ovarian cancer cell invasion |
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| DOI：10.3969/j.issn.1009-6469.2022.07.032 |
| 中文关键词: 卵巢肿瘤 微小 RNA-92a(miR-92a) Kru. |
| 英文关键词: Ovarian cancer miR-92a Kruppel-like factor 2 Invasion Target gene |
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| 摘要点击次数: 2008 |
| 全文下载次数: 1070 |
| 中文摘要: |
| 目的研究微小 RNA-92a(miR-92a)靶向 Kruppel.样因子 2(KLF2)基因促进卵巢癌细胞侵袭的作用及机制。方法 2019年 1月至 2020年 3月进行本研究。培养卵巢癌细胞系 A2780、SKOV3、OVCAR-3及卵巢上皮细胞系 HOSEpiC，检测 miR-92a的表达水平； SKOV3细胞分为阴性对照( NC)组、 miR-92a抑制物组、 miR-92a组、 pcDNA组、 pcDNA+miR-92a组、 pcDNA-KLF2+miR-92a组，分别转染 NC序列、 miR-92a、miR-92a抑制物、空白 pcDNA质粒、过表达 KLF2的 pcDNA重组质粒，检测细胞侵袭数目、 KLF2表达水平，双荧光素酶报告基因验证 miR-92a靶向 KLF2基因。结果 A2780、SKOV3、OVCAR-3细胞中 miR-92a的表达水平分别为( 0.92±0.15)、(1.62±0.19)、(1.21±0.13)，均高于 HOSEpiC的( 0.62±0.09)(P<0.05)；与 NC组( 75.38± |
| 英文摘要: |
| Objective To study the role and mechanism of microRNA-92a (miR-92a) targeting the Kruppel.-like factor 2 (KLF2) gene to promote ovarian cancer cell invasion.Method The study was conducted from January 2019 to March 2020. Ovarian cancer cell lines A2780, SKOV3, OVCAR-3 and ovarian epithelial cell line HOSEpiC were cultured to detect the expression level of miR-92a; SKOV3 cells were divided into negative control (NC) group, miR-92a inhibitor group, miR-92a group, pcDNA group, pcDNA+miR-92a group, pcDNA-KLF2+miR-92a group, transfected with NC sequence, miR-92a, miR-92a inhibitor, blank pcDNA plasmid and pcDNA recombi-nant plasmid overexpressing KLF2, respectively. The number of invaded cells and the expression level of KLF2 were detected, and a du.al-luciferase reporter gene verified that miR-92a targeted the KLF2 gene.Result The expression levels of miR-92a in A2780, SKOV3 and OVCAR-3 cells were 0.92±0.15, 1.62±0.19 and 1.21±0.13, respectively, which were higher than those in HOSEpiC cells (0.62± 0.09) (P < 0.05). Compared with the NC group (75.38±13.82), (0.42±0.08), the invasion number of SKOV3 cells in the miR-92a inhibitor group (42.38±8.79) decreased, and the expression level of KLF2 increased (0.78±0.20). In the miR-92a group, the invasion number ofSKOV3 cells increased (109.38±21.38), the expression level of KLF2 decreased (0.29±0.06), and the fluorescence activity of the wild-type KLF2 dual-luciferase reporter gene decreased (P<0.05). Compared with the pcDNA group, the invasion number of SKOV3 cells inthe pcDNA+miR-92a group increased, and compared with the pcDNA+miR-92a group, the invasion number of SKOV3 cells in the pcD-NA-KLF2+miR-92a group decreased (P < 0.05).Conclusions The expression of miR-92a is increased in ovarian cancer cells, and miR-92a can promote the invasion of SKOV3 cells. The mechanism may be related to the targeted regulation of KLF2 gene expression. |
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