| 汪化文,申仑,张绍义,等.长链非编码 RNA母系表达基因 3、微小 RNA-21表达与冠状动脉钙化的相关性分析[J].安徽医药,2022,26(10):2053-2057. |
| 长链非编码 RNA母系表达基因 3、微小 RNA-21表达与冠状动脉钙化的相关性分析 |
| Analysis of the correlation between the expressions of LncRNA MEG3 and miR-21 and coronary artery calcification |
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| DOI：10.3969/j.issn.1009-6469.2022.10.034 |
| 中文关键词: 冠状动脉疾病 血管钙化 长链非编码 RNA母系表达基因 3 微小 RNA-21 冠状动脉钙化 相关性 |
| 英文关键词: Coronary artery disease Vascular calcification Long non-coding RNA maternally expressed gene 3 MicroRNA-21 Coronary artery calcification Correlation |
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| 中文摘要: |
| 目的探究长链非编码 RNA母系表达基因 3(LncRNA MEG3)、微小 RNA-21(miR-21)与冠状动脉钙化的相关性。方法选取 2018年 6月至 2019年 6月在邯郸市中心医院行冠状动脉造影及 CT检查确诊为冠状动脉钙化病人 189例作为钙化组，另选取同期在该院行冠状动脉造影及 CT检查显示无冠状动脉钙化者 183例作为未钙化组。采用实时荧光定量 PCR(qRT-PCR)法检测血清 LncRNA MEG3、miR-21表达水平，采用 Pearson法分析病人血清 LncRNA MEG3、miR-21水平及与糖化血红蛋白(HbA1c)、高敏 C反应蛋白( hs-CRP)、肿瘤坏死因子 -α(TNF-α)、白细胞介素 -1β(IL-1β)水平相关性，采用 logistic回归模型分析冠状动脉钙化发生的影响因素，采用 ROC曲线评估血清 LncRNA MEG3、miR-21表达水平对冠状动脉钙化的预测价值。结 |
| 英文摘要: |
| Objective To investigate the relationships between long non-coding RNA maternally expressed gene 3 (LncRNA MEG3), microRNA-21 (miR-21) and coronary artery calcification.Methods From June 2018 to June 2019, 189 patients with coronaryartery calcification confirmed by coronary angiography and CT in Handan Central Hospital were selected as calcification group, in addi.tion, 183 patients who underwent coronary angiography and CT examination in the same period and showed no coronary artery calcifica.tion were selected as non-calcified group. The expression levels of serum LncRNA MEG3 and miR-21 were detected by real-time fluo. rescent quantitative PCR (qRT-PCR), Pearson's method was used to analyze the levels of serum LncRNA MEG3 and miR-21, and their correlation with glycosylated hemoglobin (HbA1c), high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α), inter. leukin-1β (IL-1β). Logistic regression model was used to analyze the influencing factors of coronary artery calcification, and ROC curvewas used to evaluate the predictive value of serum expression levels of LncRNA MEG3 and miR-21 for coronary artery calcification.Re. sults Compared with the non-calcified group, the serum miR-21 [(2.87±0.93 vs. (1.02±0.29], HbA1c [(6.95±1.31) % vs. (5.41± 1.26) %], hs-CRP [(5.13±1.74) mg/L vs. (3.02±1.01) mg/L], TNF-α [(11.72±3.92) ng/L vs. (9.82±4.13) ng/L], IL-1β [(4.96±0.95) ng/L vs. (2.32±0.74) ng/L] and the proportions of coronary heart disease [(80.95 %) vs. (15.85 %)] and hypertension [(53.97 %) vs. (22.40 %)] in the calcified group were higher (P<0.05), and the expression level of serum LncRNA MEG3 [0.62±0.17 vs. 1.01±0.31] was lower (P< 0.05). Serum LncRNA MEG3 was negatively correlated with miR-21, hs-CRP, TNF-α and IL-1β levels in patients with coronary artery calcification, and miR-21 was positively correlated with hs-CRP, TNF-α and IL-1β (P<0.05). Coronary heart disease, LncRNA MEG3 and miR-21 were the risk factors for coronary artery calcification (P<0.05). The area under the curve of the combined detection of se. rum LncRNA MEG3 and miR-21 in predicting coronary artery calcification was 0.88 (95 % CI: 0.84-0.91), the sensitivity was 84.13 %, and the specificity was 85.79 %.Conclusion The expression level of serum LncRNA MEG3 in patients with coronary artery calcifica.tion is significantly decreased, and the expression level of miR-21 is significantly increased, both of which may serve as biomarkers to commonly predict the occurrence of coronary artery calcification. |
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