| 钟红,骆勇,石志强,等.便携式近红外光谱仪的僵蚕快速检验应用研究[J].安徽医药,2022,26(12):2399-2402. |
| 便携式近红外光谱仪的僵蚕快速检验应用研究 |
| Application of portable near-infrared spectrometer in rapid detection of Bombyx Batryticatus |
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| DOI：10.3969/j.issn.1009-6469.2022.12.014 |
| 中文关键词: 蚕属 主成分分析 谱学，近红外线 微量化学 产地 炮制品 定性分析 化学计量学 |
| 英文关键词: Bombyx Principal component analysis Spectroscopy, near-infrared Microchemistry Origin Processed products Qualitative analysis Stoichiometry |
| 基金项目:四川省重大科技专项课题( 2018TZDZX0007) |
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| 摘要点击次数: 1905 |
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| 中文摘要: |
| 目的通过便携式近红外光谱仪建立快速判别僵蚕产地以及快速鉴别僵蚕与麸炒僵蚕的方法。方法 2021年 2—3月，搜集 84批不同产地僵蚕及 24批麸炒僵蚕样本，采用便携式近红外光谱仪采集样本光谱，在无监督的主成分分析( PCA)基础上，利用线性判别分析( LDA)模式识别方法并结合不同光谱预处理方法分别对僵蚕产地以及僵蚕、麸炒僵蚕建立定性分析模型。结果对光谱进行平滑预处理后建立的模型可对僵蚕 3个产地实现准确区分，校正集与验证集的判别准确率分别达到 |
| 英文摘要: |
| Objective To establish the method of fast identification of the origin of Bombyx Batryticatus and fast identification of theBombyx Batryticatus and Bombyx Batryticatus Stir-fried with bran by portable near-infrared (NIR) spectrometer.Methods From Feb. ruary to March, 2021, 84 batches of Bombyx Batryticatus samples from different producing areas and 24 batches of Bombyx Batrytica.tus Stir-fried samples with Bran were collected, spectra of samples were collected by a portable NIR spectrometer. On the basis of unsu.pervised principal component analysis (PCA), linear discriminant analysis (LDA) pattern recognition method combined with differentspectral pretreatment methods were used to establish qualitative analysis models for the origins, the Bombyx Batryticatus and the Bom.byx Batryticatus Stir-fried with Bran.Results The model established after smoothing pretreatment of the spectrum can accurately dis.tinguish the three origins of the Bombyx Batryticatus, the discriminant accuracy of calibration set and validation set reached 98.41%and 90.48%, respectively; In the model of Bombyx Batryticatus and Bombyx Batryticatus Stir-fried with Bran, the discriminant accura.cy of calibration set and validation set in the qualitative model established by all spectral pretreatment methods reached 100.00%,which could correctly identify the processing of Bombyx Batryticatus.Conclusion The portable NIR spectrometer combined with stoi.chiometry can be used to identify the origin and processed products of Bombyx Batryticatus in real time and accurately, which is ofpractical significance for the quality evaluation of Bombyx Batryticatus medicinal materials. |
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