| 郭云波,张晓萍,高崴崴,等.老年重症肺炎病人血清微小 RNA-146a、微小 RNA-223、微小 RNA-21、微小 RNA-124水平及其与预后的关系[J].安徽医药,2022,26(12):2443-2447. |
| 老年重症肺炎病人血清微小 RNA-146a、微小 RNA-223、微小 RNA-21、微小 RNA-124水平及其与预后的关系 |
| Serum levels of miR-146a, miR-223, miR-21, and miR-124 in elderly patients with severe pneumonia and their relationship with prognosis |
| |
| DOI：10.3969/j.issn.1009-6469.2022.12.024 |
| 中文关键词: 肺炎 微小 RNA-146a 微小 RNA-223 微小 RNA-21 微小 RNA-124 预后 肿瘤坏死因子 -α 老年人 |
| 英文关键词: Pneumonia MicroRNA-146a MicroRNA-223 factor-alpha Aged |
| 基金项目:河南省医学科技攻关计划项目( LHGJ20200413) |
|
| 摘要点击次数: 906 |
| 全文下载次数: 214 |
| 中文摘要: |
| 目的探究老年重症肺炎病人血清微小 RNA(miR)-146a、miR-223、miR-21、miR-124表达水平及其与预后的关系。方法选取 2018年 2月至 2020年 3月郑州大学第二附属医院诊治的 128例老年重症肺炎病人、 130例普通肺炎病人分别为重症肺炎组、普通肺炎组，并纳入同期 128例体检健康者为健康组。比较三组血清 miR-146a、miR-223、miR-21、miR-124表达水平及肿瘤坏死因子 -α(TNF-α)水平；分析老年重症肺炎病人血清 miR-146a、miR-223、miR-21、miR-124表达水平与 TNF-α的相关性；比较不同预后老年重症肺炎病人临床资料和血清 miR-146a、miR-223、miR-21、miR-124水平；分析老年重症肺炎病人死亡的影响因素；分析血清 miR-146a、miR-223、miR-21、miR-124、TNF-α对老年重症肺炎病人预后的预测价值。结果普通肺炎组病人血清 miR-146a、miR-223、miR-21表达水平及 TNF-α水平分别为 1.67±0.56、1.64±0.55、1.71±0.57、(12.78±4.30)ng/L，重症肺炎组病人血清 miR-146a、miR-223、miR-21表达水平及 TNF-α水平分别为 2.32±0.78、2.27±0.76、2.39±0.80、(23.64±7.92)ng/L，高于健康组的 1.03±0.35、1.01±0.34、0.99±0.33、(5.43±1.84)ng/L、(P<0.05)普通肺炎组、重症肺炎组血清 miR-124表达水平分别为 0.71±0.24、0.37±0.13，低于健康组的 1.07±0.37(P<0.05)；重症肺炎人血清 miR-146a、miR-223、miR-21表达水平及 TNF-α水组病，平高于普通肺炎组( P<0.05)血清 miR-124表达水平低于普通肺炎组( P<0.05)；老年重症肺炎病人血清 miR-146a、miR-223、 miR-21表达水平与 TNF-α呈正，相关( P<0.05)血清 miR-124表达水平与 TNF-α呈负相关( P<0.05)；死亡组老年重症肺炎病人血清 miR-146a、miR-223、miR-21、TNF-α水平及有，呼吸系统疾病史、吸烟史、机械通气占比高于生存组( P<0.05)，血清 miR-124表达水平、氧分压水平低于生存组( P<0.05)； miR-146a、miR-223、miR-21、TNF-α、机械通气是老年重症肺炎病人死亡的危险因素( P<0.05)；血清 miR-146a、miR-223、miR-21、miR-124、TNF-α预测老年重症肺炎病人预后的曲线下面积( AUC)分别为 0.84、0.88、0.82、0.66、0.68，其灵敏度分别为 72.5%、80.4%、82.4%、60.8%、60.8%，特异度分别为 85.7%、81.8%、79.2%、67.5%、72.4%。结论老年重症肺炎病人血清 miR-146a、miR-223、miR-21表达水平较高，与 TNF-α水平呈正相关； miR-124表达水平较低，与 TNF-α水平呈负相关。且测定血清 miR-146a、miR-223、miR-21表达水平有助于临床预测老年重症肺炎病人的预后情况。 |
| 英文摘要: |
| Objective To investigate the expression levels of serum microRNA (miR)-146a, miR-223, miR-21 and miR-124 in el. derly patients with severe pneumonia and their relationship with prognosis.Methods A total of 128 elderly patients with severe pneu.monia and 130 patients with common pneumonia who were treated at the Second Affiliated Hospital of Zhengzhou University from Feb.ruary 2018 to March 2020 were selected as the severe pneumonia group and the common pneumonia group, respectively, and 128healthy individuals with physical examination during the same period were included as the healthy group. The serum expression levelsof miR-146a, miR-223, miR-21, miR-124 and tumor necrosis factor-α (TNF-α) were compared in the three groups. We analyzed the correlation between serum miR-146a, miR-223, miR-21, miR-124 expression levels and TNF-α in elderly severe pneumonia patients; compared the clinical data and serum levels of miR-146a, miR-223, miR-21, and miR-124 in elderly severe pneumonia patients withdifferent prognoses; analyzed the influencing factors of death in elderly severe pneumonia patients; and analyzed the predictive value of serum miR-146a, miR-223, miR-21, miR-124 and TNF-α on the prognosis of elderly severe pneumonia patients.Results The expres. sion levels of serum miR-146a, miR-223, miR-21 and TNF-α in patients with common pneumonia were 1.67±0.56, 1.64±0.55, 1.71±0.57 and (12.78±4.30) ng/L, respectively, while those in patients with severe pneumonia were 2.32±0.78, 2.27±0.76, 2.39±0.80 and(23.64±7.92) ng/L, respectively, which were higher than those in the healthy group [1.03±0.35, 1.01±0.34, 0.99±0.33, (5.43±1.84) ng/L] (P<0.05). The expression levels of serum miR-124 in the common pneumonia group and severe pneumonia group were 0.71±0.24and 0.37±0.13, respectively, which were lower than those in the healthy group (1.07±0.37) (P<0.05). The expression levels of serum miR-146a, miR-223, miR-21 and TNF-α in the severe pneumonia group were higher than those in the common pneumonia group (P< 0.05), and the expression level of serum miR-124 was lower than that in the common pneumonia group (P<0.05). The expression levels of serum miR-146a, miR-223, and miR-21 in elderly severe pneumonia patients were positively correlated with TNF-α(P<0.05), and the expression level of serum miR-124 was negatively correlated with TNF-α(P<0.05). The expression levels of serum miR-146a, miR-223, miR-21, and TNF-α and the history of respiratory diseases, smoking history, and proportion of mechanical ventilation of elderly se.vere pneumonia patients in the death group were higher than those in the survival group (P<0.05), and the expression level of serum miR-124 and the level of oxygen partial pressure were lower than those in the survival group (P<0.05). The miR-146a, miR-223, miR-21, TNF-α, and mechanical ventilation were risk factors for death in elderly severe pneumonia patients (P<0.05). The areas under the curve (AUCs) of serum miR-146a, miR-223, miR-21, miR-124, and TNF-α for predicting prognosis in elderly severe pneumonia pa.tients were 0.84, 0.88, 0.82, 0.66, and 0.68, respectively, with sensitivities of 72.5%, 80.4%, 82.4%, 60.8% and 60.8%, respectively,and specificities were 85.7%, 81.8%, 79.2%, 67.5% and 72.4%, respectively.Conclusions The expression levels of serum miR-146a, miR-223 and miR-21 in elderly patients with severe pneumonia were higher and positively correlated with TNF-α levels; the expression level of miR-124 was lower and negatively correlated with TNF-α levels. The measurement of serum miR-146a, miR-223 and miR-21 expression levels could help to predict the prognosis of elderly patients with severe pneumonia clinically. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
