| 李婷君,王晶.从 Janus激酶 2/信号转导和转录激活因子 3信号通路探讨牛磺酸对 Aβ25-35所致神经干细胞损伤的保护作用[J].安徽医药,2023,27(1):30-36. |
| 从 Janus激酶 2/信号转导和转录激活因子 3信号通路探讨牛磺酸对 Aβ25-35所致神经干细胞损伤的保护作用 |
| Exploring the protective effect of taurine on neural stem cell injury induced by Aβ25-35 based on Janus kinase 2/ signal transducer and activator of transcription 3 signal pathway |
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| DOI：10.3969/j.issn.1009-6469.2023.01.007 |
| 中文关键词: 牛磺酸 阿尔茨海默病 神经干细胞 增殖分化 凋亡 Janus激酶 2/信号转导和转录激活因子 3信号通路 |
| 英文关键词: TTaurine Alzheimer′s disease Neural stem cells Proliferation and differentiation Apoptosis JAK2/STAT3 signaling pathway |
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| 中文摘要: |
| 目的探讨牛磺酸对 Aβ25-35损伤神经干细胞( neural stem cells，NSCs)的保护作用，并初步阐明其作用机制。方法研究自 2019年 3—11月通过 Aβ25-35损伤实验室提取的神经干细胞模型，探讨牛磺酸和 Janus激酶 2/信号转导和转录激活因子3该(JAK2/STAT3)的作用关系。从出生 48 h内的 C57BL/6乳鼠海马区提取 NSCs，以 25 μmol/L浓度 Aβ25-35诱导 NSCs，建立体外阿尔茨海默病( Alzheimer’s disease，AD)样 NSCs模型( AD-NSCs)，并利用不同浓度的牛磺酸共同孵育，分为对照组、模型组( 25 μmol/L)、 Aβ25-35+牛磺酸 5 mmol组、 Aβ25-35+牛磺酸 10 mmol组、 Aβ25-35+牛磺酸 15 mmol组及 Aβ25-35+牛磺酸 20 mmol组，共六组。 |
| 英文摘要: |
| Objective To explore the protective effect of taurine on neural stem cells (NSCs) injury caused by Aβ25-35 and its possible mechanism.Methods The relationship between taurine and Janus kinase 2/ signal transducer and activator of transcription 3(JAK2/STAT3) was investigated by Aβ25-35 injury neural stem cell model from March 2019 to November 2019. NSCs were extracted fromthe hippocampus of C57BL/6 mice within 48 hours of birth, and NSCs were incubated with 25 μmol/L Aβ25-35 to establish an in vitro model of Alzheimer′s disease (AD) and incubation with a range of concentrations of taurine, which were assigned into control group,model group (25 μmol/L Aβ25-35), Aβ25-35+taurine 5 mmol group, Aβ25-35+taurine 10 mmol group, and Aβ25-35+Taurine 15 mmol group and Aβ25-35+Taurine 20 mmol group, a total of 6 groups. The viability of NSCs were detected by CCK-8 assay. The proliferation ability ofNSCs were measured by cell sphere radius measurement and EDU staining. The differentiation ability of NSCs were observed by immunofluorescence staining. The expression of apoptosis-related genes Bax, Bcl-2 and caspase-3 mRNA was detected by Real-time PCR. The expression of t-JAK2, t-STAT3, p-JAK2 and p-STAT3 proteins were detected by Western blotting.Results Compared with thecontrol group (100.00%), the vitality of NSCs in the model group [(61.25+6.36)%] was significantly decreased. The radius of the cellsphere and proliferation ratio decreased significantly. The proportion of NSCs differentiating into neurons decreased. Bax/Bcl-2 ratio and caspase-3 mRNA expression were significantly increased. The protein expressions of p-JAK2 and p-STAT3 were significantly increased, and the differences were statistically significant compared with the control group (P<0.05). Compared with the model group[(61.25+6.36)%], each concentration of taurine [(74.82±5.14)%, (81.58±7.03)%, (74.32±8.27)%, (69.33±4.36)%] significantly improved the vitality of AD-NSCs; significantly increased the radius of the cell sphere and promoted its proliferation; significantly promoted the differentiation of NSCs into neurons; significantly reduced Bax/Bcl-2 ratio and caspase-3 mRNA expression; in addition, taurine cold also inhibited the expression of p-JAK2 and p-STAT3 protein. Compared with the model group, the above indicators have statistically significant differences (P<0.05).Conclusion Taurine had a certain protective effect on NSCs caused by Aβ25-35, and its mechanism may be related to the inhibition of JAK2/STAT3 signaling pathway. |
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