| 祝仰廷,李刚,孙伟,等.黄芪通过微小 RNA-133a抑制 Janus激酶 /信号转导和转录激活因子信号通路抑制膀胱癌的迁移和侵袭[J].安徽医药,2023,27(1):55-59. |
| 黄芪通过微小 RNA-133a抑制 Janus激酶 /信号转导和转录激活因子信号通路抑制膀胱癌的迁移和侵袭 |
| Astragalus affects the migration and invasion of bladder cancer through microRNA-133a (miR-133a) inhibition of Janus kinase (JAK) / signal transducer and activator of transcription (STAT) signaling pathway |
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| DOI：10.3969/j.issn.1009-6469.2023.01.012 |
| 中文关键词: 黄芪 膀胱肿瘤 微小 RNA-133a JAK/STAT信号通路 迁移 侵袭 |
| 英文关键词: Astragalus Urinary bladder neoplasm miR-133a JAK/STAT signaling pathway Migration Invasion |
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| 中文摘要: |
| 目的探讨黄芪对膀胱癌细胞迁移和侵袭的影响及分子机制。方法 2019年 8月至 2020年 2月，从美国模式培养物研究所( ATCC)购入膀胱癌 T24细胞，体外培养并分为对照( NC)组、黄芪组、 miR-133a模拟物( mimic)阴性对照( miR-NC)组、 miR-133a mimic(miR-133a)组、 miR-133a抑制剂( inhibitor)阴性对照( anti-miR-NC)组、 miR-133a inhibitor(anti-miR-133a)组、芪+anti-miR-NC组、黄芪 +anti-miR-133a组、黄芪 +anti-miR-133a+AG490组。蛋白质印迹法( western blotting)检测基质金属蛋白黄酶-2(MMP-2)、基质金属蛋白酶 -9(MMP-9)磷酸化 Janus激酶 1(p-JAK1)、磷酸化信号转导和转录激活因子 6(p-STAT6)蛋白表达； Transwell检测细胞迁移和侵袭；实时荧光、定量 PCR(RT-qPCR)检测 miR-133a表达水平。结果与对照组相比，黄芪组 MMP-2、MMP-9蛋白表达水平降低，细胞迁移［(81.42±8.05)比( 175.43±16.02)］和侵袭［(71.23±8.01)比( 142.55±14.03)］数量降低， miR-133a表达水平［( 2.36±0.21)比( 1.00±0.11)］升高， p-JAK1［( 0.12±0.01)比( 0.55±0.04)］、 p-STAT6［( 0.08±0.01)比( 0.38± |
| 英文摘要: |
| Objective To explore the effect and molecular mechanism of Astragalus on the migration and invasion of bladder cancer cells.Methods From August 2019 to February 2020, bladder cancer T24 cells were purchased from ATCC in the United States. Thecells were cultured in vitro and assigned into control (NC) group, Astragalus group, miR-133a mimic negative control (miR-NC) group, miR-133a mimic (miR-133a) group, miR-133a inhibitor negative control (anti-miR-NC) group, miR-133a inhibitor (anti-miR-133a) group, Astragalus+anti-miR-NC group, Astragalus+anti-miR-133a group, Astragalus +anti-miR-133a+AG490 group. Western blotting were adopted for the detection of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), phosphorylated Janus kinase 1 (p-JAK1), phosphorylated signal transducer and activator of transcription-6 (p-STAT6) protein expressions; Transwell was adopted for the detection of cell migration and invasion, and real-time fluorescence quantitative PCR (RT-qPCR) for the detection of miR133a expression.Results Compared with the control group, the expressions of MMP-2 and MMP-9 protein in the Astragalus group were decreased, the number of cell migration [(81.42±8.05) vs. (175.43±16.02)] and invasion [(71.23±8.01) vs. (142.55±14.03)] was decreased, the expression of miR-133a [(2.36±0.21) vs. (1.00±0.11)] was increased, and the protein expressions of p-JAK1 [(0.12±0.01) vs. (0.55±0.04)] and p-STAT6 [(0.08±0.01) vs. (0.38±0.03)] were decreased (P<0.05). Overexpression of miR-133a inhibited the migration [(105.11±9.86) vs. (171.16±15.04)] and invasion [(107.35±9.67) vs. (139.04±12.37)] of bladder cancer cell T24; inhibition of miR133a expression achieved opposite results. Inhibition of miR-133a expression can reverse the inhibitory effect of Astragalus on migration and invasion of T24 cells. Blocking the JAK / STAT signaling pathway can reverse the effects of inhibitory miR-133a expression combined with Astragalus on migration and invasion of T24 cells.Conclusion Astragalus may inhibit the migration and invasion of bladder cancer cells by up-regulating miR-133a expression and inhibiting JAK / STAT signaling pathway. |
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