| 王帅,朱超男,邬鹏宇,等.萝卜硫素对心肌缺血再灌注损伤大鼠心肌保护作用及色氨酸 -犬尿氨酸通路的影响[J].安徽医药,2023,27(1):60-64. |
| 萝卜硫素对心肌缺血再灌注损伤大鼠心肌保护作用及色氨酸 -犬尿氨酸通路的影响 |
| Effects of sulforaphane on myocardial protection and tryptophan-kynurenine pathway in rats with myocardial ischemia reperfusion injury |
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| DOI：10.3969/j.issn.1009-6469.2023.01.013 |
| 中文关键词: 再灌注损伤 莱菔硫素 心肌缺血 色氨酸 -犬尿氨酸信号通路 bcl-2相关 X蛋白质 胱天蛋白酶 3 白细胞介素类 肿瘤坏死因子 α |
| 英文关键词: Reperfusion injury Sulforaphane Myocardial ischemia Tryptophan-kynurenine signal Bcl-2-associated X protein Caspase 3 Interleukins Tumor necrosis factor-alpha |
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| 中文摘要: |
| 目的探究萝卜硫素( SFN)对心肌缺血再灌注( I/R)损伤大鼠色氨酸( TRP)-犬尿氨酸( KYN)信号通路及心肌损伤的影响。方法研究于 2019年 7月至 2020年 6月进行，将 SD雄性大鼠随机分为假手术组( Control)、模型组( I/R)、 SFN低( I/R+L SFN)、高( I/R+H SFN)剂量组。术后 24 h，ELISA检测各组大鼠血清中炎性因子白细胞介素( IL)-1β、IL-6、肿瘤坏死因子 α(TNF-α)含量； HE染色观察各组大鼠心肌组织病理变化； TUNEL检测各组大鼠缺血心肌细胞凋亡情况； HPLC检测各组大鼠心肌组织中 TRP、KYN浓度；蛋白质印迹法( Western blotting)检测各组大鼠心肌组织 B细胞淋巴瘤 -2相关 X蛋白( Bax)、 B细胞淋巴瘤 -2(Bcl-2)、活化胱天蛋白酶 3(cleaved -capase3)、吲哚胺 2，3-双加氧酶( IDO)蛋白表达情况。结果与 Control组相比， I/R大鼠心肌细胞破碎、坏死，细胞排列不规则，心肌纤维断裂，伴有炎性细胞浸润，血清中炎性因子 IL-1β、IL-6、TNF-α含量、心肌细胞凋亡指数［( 32.85±3.13)比( 1.07±0.08)］、心肌组织 TRP［( 783.75±30.32)pmol/L比( 402.23±23.55)pmol/L］、 KYN浓度［(567.54±28.18)pmol/L比( 215.31±20.03)pmol/L］、 KYN/TRP比值、 cleaved-capase3、Bax、IDO蛋白水平显著升高( P<0.05)，Bcl-2蛋白水平显著降低(P<0.05)；与 I/R相比， I/R+L SFN、I/R+H SFN剂量组心肌细胞上述病理症状明显减轻，大鼠血清中炎性因子 IL-1β、IL-6、TNF-α含量、心肌细胞凋亡指数、心肌组织 TRP、KYN浓度、 KYN/TRP比值、 Bax、cleaved -capase3、IDO蛋白水平依次降低(P<0.05)，Bcl-2蛋白水平显著升高( P<0.05)。结论 SFN可减少心肌细胞凋亡，缓解机体炎症反应，对心肌损伤有保护作用可能与 TRP-KYN信号通路有关。 |
| 英文摘要: |
| Objective To explore the effect of sulforaphane (SFN) on the tryptophan (TRP)-kynurenine (KYN) signaling pathway and myocardial injury in rats with myocardial ischemia reperfusion (I/R) injury.Methods From July 2019 to June 2020, SD male ratswere randomly assigned into sham operation group (Control), model group (I/R), SFN low group (I/R+L SFN), and high group (I/R+HSFN) dose groups. 24h after operation, the serum levels of inflammatory factors interleukin 1β (IL-1β), interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) in each group were detected by ELISA; HE staining was used to observe the pathological changes of rat myocardial tissue; TUNEL was used to detect the apoptosis of ischemic myocardial cells in each group; HPLC was used to detect the concentration of TRP and KYN in rat myocardial tissue; western blot was used to detect the protein expression of B cell lymphoma-2 associated X protein (Bax), B cell lymphoma-2 (Bcl-2), cleaved cysteine aspartate proteolytic enzyme 3 (cleaved-capase3) and indoleamine 2, 3-dioxygenase (IDO) in the myocardial tissue of each group.Results Compared with the control group, myocardial cells of I/R ratswere broken and necrotic, the cell arrangement was irregular, myocardial fibers were broken, accompanied by inflammatory cell infiltration, and the serum levels of inflammatory factors IL-1β, IL-6 and TNF-α, myocardial cell apoptosis index [(32.85±3.13) vs. (1.07± 0.08)], myocardial tissue TRP [(783.75±30.32) pmol/L vs. (402.23±23.55) pmol/L], KYN concentration [(567.54±28.18) pmol/L vs. (215.31±20.03) pmol/L], KYN/TRP ratio, cleaved-capase3, Bax, IDO protein levels were significantly increased (P<0.05), Bcl-2 protein levels were significantly reduced (P<0.05); Compared with I/R, I/R+L SFN and I/R+H SFN dose groups have significantly reduced the above-mentioned pathological symptoms of cardiomyocytes, the serum levels of inflammatory factors IL-1β, IL-6, and TNF-α cardiomyocyte apoptosis index, myocardial tissue TRP, KYN concentration, KYN/TRP ratio, Bax, cleaved-capase3, IDO protein levels were decreased (P<0.05), but the Bcl-2 protein levels was increased significantly (P<0.05).Conclusion SFN can reduce myocardial cellapoptosis, alleviate the body′s inflammatory response, and have a protective effect on myocardial injury, which may be related to theTRP-KYN signaling pathway. |
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