| 弓翠屏,王英.洛伐他汀调控转化生长因子 -β诱导的卵巢癌细胞增殖、侵袭和迁移的机制研究[J].安徽医药,2023,27(1):69-73. |
| 洛伐他汀调控转化生长因子 -β诱导的卵巢癌细胞增殖、侵袭和迁移的机制研究 |
| Lovastatin regulates TGF-β-induced proliferation, invasion and migration of ovarian cancer cells |
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| DOI：10.3969/j.issn.1009-6469.2023.01.015 |
| 中文关键词: 卵巢肿瘤 洛伐他汀 增殖 侵袭 迁移 p38丝裂原活化蛋白激酶类 转化生长因子 β |
| 英文关键词: Ovarian neoplasms Lovastatin Proliferation Invasion Migration p38 mitogen-activated protein kinases(p38MAPK) Transforming growth factor beta |
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| 目的研究洛伐他汀对卵巢癌 SKOV3细胞迁移、侵袭的影响及作用机制。方法该研究于 2019年 8月至 2020年 5月进行，体外培养 SKOV3细胞。细胞计数( CCK-8)法检测( 0、5、10、20、40、60 μmol/L)洛伐他汀对转化生长因子 -β(TGF-β)诱导的 SKOV3细胞增殖的影响；细胞划痕、 Transwell实验、蛋白质印迹法( Western blotting)检测对照组、 TGF-β(10 μg/L)组、洛伐他汀(10 μmol/L)组、洛伐他汀 +TGF-β组 SKOV3细胞的迁移、侵袭、上皮细胞间充质化(EMT)和 p38丝裂原活化蛋白激酶(p38MAPK)信号通路蛋白水平。结果与 0 μmol/L组( 0.885±0.066、1.365±0.114、1.669±0.123)相比， 5(0.746±0.059、1.365±0.114、1.669± 0.123)、 10(0.625±0.051、1.015±0.062、1.284±0.086)、 20(0.518±0.044、0.856±0.067、1.101±0.081)、 40(0.402±0.033、0.703±0.062、 0.917±0.072)、60 μmol/L(0.276±0.022、0.536±0.043、0.756±0.071)洛伐他汀呈浓度依赖性抑制 TGF-β诱导的 SKOV3细胞 24 h、48 h和 72 h增殖( P<0.05)；与 TGF-β(10 μg/L)组相比，洛伐他汀逆转 TGF-β诱导的 SKOV3细胞迁移( 212.341±20.523比 357.962±32.632)、侵袭(114.362±10.963比 179.635±15.432)抑制 EMT，减少磷酸化 p38(p-p38)蛋白水平(1.256±0.116比 1.897±0.145)(P<0.05)。结论洛伐他汀可能通过调控 p38MAPK通路抑制 TGF-β诱导的 SKOV3细胞迁移、侵袭、 EMT。 |
| 英文摘要: |
| Objective To study the effects and mechanism of lovastatin on migration and invasion of ovarian cancer cells SKOV3.Methods In this study, SKOV3 cells were cultured in vitro from August 2019 to May 2020. The effect of lovastatin of 0, 5, 10, 20, 40,60 μmol/L on the proliferation of transforming growth factor-β (TGF-β)-induced SKOV3 cells were detected by cell counting kit-8(CCK-8). The migration and invasion of SKOV3 cells, epithelial-mesenchymal transition (EMT) and the protein level of p38 mitogen-activated protein kinases (p38MAPK) signaling pathway in the control group, TGF-β (10 μg/L) group, lovastatin (10 μmol/L) group and lovastatin+TGF-β group were detected by cell scratch test, Transwell and Western bloting assay.Results Compared with 0 μmol/L(0.885±0.066, 1.365±0.114, 1.669±0.123) group, lovastatin of 5 (0.746±0.059, 1.365±0.114, 1.669±0.123), 10 (0.625±0.051, 1.015±0.062, 1.284±0.086), 20 (0.518±0.044, 0.856±0.067, 1.101±0.081), 40 (0.402±0.033, 0.703±0.062, 0.917±0.072), 60 μmol/L (0.276±0.022, 0.536±0.043, 0.756±0.071) groups inhibited the proliferation of SKOV3 cells induced by TGF-β at 24 h, 48 h and 72 h in a concentration-dependent manner (P<0.05). Compared with the TGF-β (10 μg/L) group, lovastatin reversed the migration (212.341±20.523 vs. 357.962±32.632) and invasion (114.362±10.963 vs. 179.635±15.432) of SKOV3 cells induced by TGF-β, inhibited EMT and decreased the phosphorylated p38 (p-p38) protein level (1.256±0.116 vs. 1.897±0.145) (P<0.05).Conclusion Lovastatin may inhibit the migration, invasion and EMT of TGF-β-induced SKOV3 cells by regulating p38MAPK signaling pathway. |
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