| 钟新丽,何秋,朱红霞.孕妇外周血微小 RNA异常表达谱的鉴定及对妊娠期糖尿病的诊断价值研究[J].安徽医药,2023,27(1):117-121. |
| 孕妇外周血微小 RNA异常表达谱的鉴定及对妊娠期糖尿病的诊断价值研究 |
| Identification of abnormal expression profile of miRNA in peripheral blood and its diagnostic value for gestational diabetes mellitus |
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| DOI：10.3969/j.issn.1009-6469.2023.01.026 |
| 中文关键词: 糖尿病，妊娠 微小 RNA 高通量筛选分析 外周血 |
| 英文关键词: Diabetes, gestational miRNA High-throughput screening assays Peripheral blood |
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| 中文摘要: |
| 目的对孕妇外周血微小 RNA(miRNA)异常表达谱进行鉴定并探讨对妊娠期糖尿病( GDM)的诊断价值。方法择 2018年 1月至 2020年 2月成都市双流区第一人民医院收治的 62例 GDM孕妇( GDM组)及 62例糖耐量正常( NGT)的孕妇选(NGT组)为研究对象，在妊娠晚期( 26~40周)收集孕妇外周血，利用最新高通量测序技术鉴定外周血中差异性表达的 miRNAs，利用 RT-PCR验证鉴定的靶点。统计分析筛选的差异性 miRNA与临床参数的相关性及对 GDM发生的影响。结果与 NGT组相比， GDM组外周血共检测到 157个差异表达的 miRNAs，其中 114个表达上调， 43个表达下调。层级聚类显示 GDM组与 NGT组的 miRNA图谱明显不同，其中 8个 miRNAs显著上调， 6个 miRNAs显著下调。利用 qPCR验证在外周中高表达且最可能与能量代谢相关的差异表达的 miRNAs，qPCR结果显示 NGT组、 GDM组 miRNA-125b的相对表达水平分别为 5.83±1.31、 |
| 英文摘要: |
| Objective To identify the abnormal expression profiles of miRNA in peripheral blood of pregnant women and explorethe diagnostic value of gestational diabetes mellitus (GDM).Methods Sixty-two pregnant women with GDM (GDM group) and 62 pregnant women with normal glucose tolerance (NGT group) admitted to the First People′s Hospital of Shuangliu District of Chengdu fromJanuary 2018 to February 2020 were selected as subjects. The peripheral blood of pregnant women was collected in the third trimesterof pregnancy (26-40 weeks). The differential expression of miRNAs in the peripheral blood was identified by the latest high-throughput sequencing technology, and the identified targets were verified by RT-PCR. The correlation between differential miRNA and clinical parameters and its effect on the occurrence of GDM were statistically analyzed.Results Compared with the NGT group, 157 differentially expressed miRNAs were detected in peripheral blood of GDM group, of which 114 were up-regulated and 43 were down regulated. Hierarchical clustering showed that the miRNA profiles of GDM group and NGT group were significantly different, with 8 miRNAssignificantly up-regulated and 6 miRNAs significantly down-regulated. QPCR was used to verify the differentially expressed miRNAshighly expressed in the periphery and most likely related to energy metabolism. QPCR results showed that the relative expression levelsof miRNA-125b [(5.83 ± 1.31) vs. (1.34 ± 0.30)], miRNA-543 [(0.93 ± 0.21) vs. (0.22 ± 0.06)] and miRNA-144 [(0.42 ± 0.19) vs. (1.32 ±0.28)] in the NGT and GDM groups were consistent with the next generation sequencing results. Compared with non pregnant women with gestational diabetes mellitus, the expression of miRNA-125b in peripheral blood of pregnant women with gestational diabetes mellitus was down regulated (P<0.001), and the expression of miRNA-144 was up-regulated (P<0.001). Multivariate analysis showed that miRNA-125b, miRNA-144 and pre pregnancy body mass index were independent risk factors for GDM (P < 0.5).Conclusion In the peripheral blood of GDM patients, the expression of miRNA-125b and miRNA-144 is in a state of imbalance, which has a good diagnostic value for gestational diabetes mellitus. |
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