| 乔超锋,靳磊,宁涛,等.血清中丝氨酸肽酶抑制剂Kazal 4 型联合癌胚抗原、糖类抗原199 对结直肠癌的诊断价值[J].安徽医药,2023,27(3):550-554. |
| 血清中丝氨酸肽酶抑制剂Kazal 4 型联合癌胚抗原、糖类抗原199 对结直肠癌的诊断价值 |
| Diagnostic value of serum serine peptidase inhibitor Kazal-type 4 combined with carcinoembryonic antigen and CA199 for colorectal cancer |
| |
| DOI：10.3969/j.issn.1009-6469.2023.03.028 |
| 中文关键词: 结直肠肿瘤 丝氨酸蛋白酶抑制剂 丝氨酸肽酶抑制剂Kazal 4型 癌胚抗原 糖类抗原199 癌症早期检测 |
| 英文关键词: Colorectal neoplasms Serine proteinase inhibitors Serine peptidase inhibitor Kazal-type 4 Carcinoembryonic an?tigen Carbohydrate antigen 199 Early detection of cancer |
| 基金项目:2018年度河南理工大学青年基金资助项目(Q2018-6) |
|
| 摘要点击次数: 556 |
| 全文下载次数: 196 |
| 中文摘要: |
| 目的 检测外周血中丝氨酸肽酶抑制剂Kazal 4型(SPINK4)的水平，及联合癌胚抗原、糖类抗原199(CA199)对结直肠癌的诊断价值。方法 收集2020年1月至2021年7月在焦作市第二人民医院就诊的结直肠癌病人85例，及同时期来该院体检的健康志愿者45例。酶联免疫吸附测定(ELISA)检测病人外周血中SPINK4、癌胚抗原和糖类抗原199(CA199)的水平，比较SPINK4、癌胚抗原和CA199的表达水平与结直肠癌临床病理特征的相关性，受试者操作特征曲线(ROC曲线)评估SPINK4、癌胚抗原和CA199单独及联合检测诊断结直肠癌的临床价值。结果 结直肠癌组外周血中SPINK4(15.84±2.10)μg/L、癌胚抗原(24.62±4.81)μg/L和CA199(58.10±6.62)U/mL的水平均高于健康对照组(2.85±0.54)μg/L、(1.02±0.08)μg/L和(18.36±4.08)U/mL，结直肠癌术后外周血中SPINK4的水平为10.25±1.84，与术前相比显著降低，组间比较均差异有统计学意义(P<0.05)。SPINK4表达水平与病人年龄、肿瘤长径、分化程度、TNM分期、脉管浸润和淋巴结转移有关，癌胚抗原表达水平与病人分化程度和TNM分期有关，CA199表达水平与病人分化程度、TNM分期、脉管浸润和淋巴结转移有关。SPINK4+癌胚抗原+CA199联合检测诊断结直肠癌的曲线下面积0.943 和准确度89.2% 均高于SPINK4(0.764、70.0%)、癌胚抗原(0.644、77.4%)、CA199(0.674、63.1%)的单独检测。结论 SPINK4和癌胚抗原在结直肠癌病人外周血中表达水平增加，与CA199联合检测可提高诊断结直肠癌的准确度，具有一定的临床应用价值。 |
| 英文摘要: |
| Objective To detect the level of serine peptidase inhibitor Kazal 4 (SPINK4) in peripheral blood and the diagnostic val?ue of combined carcinoembryonic antigen and carbohydrate antigen 199 (CA199) for colorectal cancer.Methods Eighty-five colorec?tal cancer (CRC) patients who were treated in the Second People's Hospital of Jiaozuo City from January 2020 to July 2021 and 45 healthy volunteers who came to the hospital for physical examination during the same period were collected. Enzyme-linked immunosor?bent assay (ELISA) detected the levels of SPINK4, CA199 in the peripheral blood of patients, and the correlation between the expres?sion levels of SPINK4, carcinoembryonic antigen and CA199 and the clinicopathological characteristics of CRC was compared. Receiv?er operating characteristic curve (ROC curve) evaluated the clinical value of SPINK4, carcinoembryonic antigen and CA199 alone or combination in the diagnosis of CRC.Results The levels of SPINK4 (15.84±2.10) μg/L, carcinoembryonic antigen (24.62±4.81) μg/Land CA199 (58.10±6.62) U/mL in the peripheral blood of the CRC group were higher than those of the healthy control group (2.85±0.54) μg/L , (1.02±0.08) μg/L and (18.36±4.08) U/mL, the level of SPINK4 in peripheral blood after CRC was 10.25±1.84, which was significantly lower than that before surgery, and there were statistical differences between groups (P<0.05). The expression level of SPINK4 was related to the patient's age, tumor diameter, degree of differentiation, TNM stage, vascular invasion and lymph node metas?tasis. The expression level of carcinoembryonic antigen was related to the degree of differentiation of patients and TNM stage. The ex?pression level of CA199 was related to the degree of differentiation, TNM stage, vascular infiltration and lymph node metastasis. The ar?ea under the curve of the combined detection of SPINK4+carcinoembryonic antigen+CA199 was 0.943 and the accuracy was 89.2% for the diagnosis of CRC, which were higher than those of SPINK4 (0.764, 70.0%), carcinoembryonic antigen (0.644, 77.4%), and CA199(0.674, 63.1%) alone.Conclusion The expression level of SPINK4 and carcinoembryonic antigen in the peripheral blood of patients with CRC are increased, combine detection with CA199 can improve the accuracy of diagnosing CRC and has certain clinical applica?tion value. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
