| 胡海峰,张弦,王水俐.尿毒症血液透析病人并发动脉粥样硬化与血清高迁移率族蛋白B1、中性粒细胞胞外诱捕网的关系[J].安徽医药,2023,27(3):571-575. |
| 尿毒症血液透析病人并发动脉粥样硬化与血清高迁移率族蛋白B1、中性粒细胞胞外诱捕网的关系 |
| Relationship between atherosclerosis in uremic hemodialysis patients and serum high mobility group protein box-1, neutrophil extracellular traps |
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| DOI：10.3969/j.issn.1009-6469.2023.03.033 |
| 中文关键词: 尿毒症 血液透析 高迁移率族蛋白B1 中性粒细胞胞外诱捕网 动脉粥样硬化 |
| 英文关键词: Uremia Hemodialysis High mobility group protein box-1 Neutrophil extracellular traps Atherosclerosis |
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| 中文摘要: |
| 目的 探讨尿毒症血液透析病人并发动脉粥样硬化与血清高迁移率族蛋白B1(HMGB1)、中性粒细胞胞外诱捕网(NETs)表达水平的关系。方法 选取2019年1月至2020年1月上海杨思医院收治的225例尿毒症血液透析病人为研究对象，根据是否并发动脉粥样硬化将其分为非动脉粥样硬化组119例、动脉粥样硬化组106例。采用酶联免疫吸附测定(ELISA)检测血清HMGB1水平，采用荧光染色法检测血清NETs水平，采用彩色多普勒超声诊断仪检测内膜中层厚度(IMT)，采用全自动生化分析仪检测生化指标钙、磷、三酰甘油、总胆固醇、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平，并计算钙磷乘积；分析尿毒症血液透析合并动脉粥样硬化病人血清HMGB1、NETs与IMT及生化指标的相关性；分析影响尿毒症血液透析病人动脉粥样硬化发生的因素。结果 动脉粥样硬化组HMGB1［(8.94±2.33)μg/L 比(6.21±1.16)μg/L］、NETs［(416.82±45.61)μg/L比(194.58±20.23)μg/L］、IMT、三酰甘油、总胆固醇、LDL-C水平及年龄高于非动脉粥样硬化组，HDL-C水平低于非动脉粥样硬化组(P<0.05)。尿毒症血液透析合并动脉粥样硬化病人血清HMGB1 与NETs 呈正相关(r=0.54，P<0.001)；尿毒症血液透析合并动脉粥样硬化病人血清HMGB1、NETs与IMT、三酰甘油、总胆固醇、LDL-C呈正相关，与HDL-C呈负相关(P<0.05)。HMGB1、NETs是影响尿毒症血液透析病人并发动脉粥样硬化的独立危险因素(P<0.05)。结论 并发动脉粥样硬化的尿毒症血液透析病人血清HMGB1、NETs表达水平升高，二者对预测尿毒症血液透析病人动脉粥样硬化有重要意义。 |
| 英文摘要: |
| Objective To investigate the relationship between atherosclerosis in uremic hemodialysis patients and the expression levels of serum high mobility group protein box-1 (HMGB1) and neutrophil extracellular traps (NETs).Methods A total of 225 cases of uremic hemodialysis patients in Shanghai Yangsi Hospital from January 2019 to January 2020 were selected as the research objects,and they were divided into non atherosclerotic group (119 cases) and atherosclerotic group (106 cases) according to whether they were complicated with atherosclerosis. The level of serum HMGB1 was detected by enzyme-linked immunosorbent assay (ELISA), the level of serum NETs was detected by fluorescence staining, the intima-media thickness (IMT) was measured by color Doppler ultrasound, the levels of calcium, phosphorus, triglyceride, total cholesterol, low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) were detected by automatic biochemical analyzer, and the calcium phosphorus product was calculated; To analyze the correlation between serum HMGB1, NETs, IMT and biochemical indexes in uremic hemodialysis patients with atherosclerosis; To analyze the influencing factors of atherosclerosis in uremic hemodialysis patients.Results The levels of HMGB1 [(8.94±2.33) μg/L vs.(6.21±1.16) μg/L], NETs [(416.82±45.61) μg/L vs. (194.58±20.23) μg/L], IMT, triglyceride, total cholesterol, LDL-C and age in athero?sclerosis group were higher than those in non atherosclerosis group, and the level of HDL-C was lower than that in non atherosclerosis group (P < 0.05). There was a positive correlation between HMGB1 and NETs in uremic hemodialysis patients with atherosclerosis (r =0.54, P < 0.001); serum HMGB1 and NETs were positively correlated with IMT, triglyceride, total cholesterol and LDL-C, and negative?ly correlated with HDL-C in uremic hemodialysis patients with atherosclerosis (P < 0.05). HMGB1 and NETs were independent risk factors of atherosclerosis in uremic hemodialysis patients (P < 0.05).Conclusion The expression levels of HMGB1 and NETs in ure?mic hemodialysis patients with atherosclerosis are increased, which are of great significance for the prediction of atherosclerosis in ure?mic hemodialysis patients. |
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