| 潘名志,徐晓文,陈峰,等.单核细胞趋化蛋白 1、颗粒蛋白前体、胶质细胞源性神经营养因子水平与阿尔茨海默病认知功能、日常生活能力相关性分析[J].安徽医药,2023,27(5):911-915. |
| 单核细胞趋化蛋白 1、颗粒蛋白前体、胶质细胞源性神经营养因子水平与阿尔茨海默病认知功能、日常生活能力相关性分析 |
| Study on serum MCP-1, PGRN and GDNF levels and diagnostic value in patients with Alzheimer's disease |
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| DOI：10.3969/j.issn.1009-6469.2023.05.013 |
| 中文关键词: 阿尔茨海默病 单核细胞趋化蛋白 1 颗粒蛋白前体 胶质细胞源性神经营养因子 认知功能 日常生活能力 |
| 英文关键词: Alzheimer's disease MCP-1 PGRN GDNF Cognitive function Ability of daily living |
| 基金项目:苏州市精神疾病临床医学中心项目( Szzx201509)；苏州市科技计划临床试验机构能力提升项目( SLT2021013) |
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| 中文摘要: |
| 目的分析血清单核细胞趋化蛋白 1(MCP-1)颗粒蛋白前体( PGRN)、胶质细胞源性神经营养因子( GDNF)水平与阿尔茨海默病( AD)病人认知功能及日常生活能力的相关性，、探讨其在 AD中的诊断价值。方法选取 2019年 7月至 2021年 6月入住苏州大学附属广济医院治疗的 41例 AD病人作为观察组，采用随机数字表法选取苏州大学附属广济医院同期经评估符合入组的 41例健康志愿者作为对照组，运用酶联免疫吸附测定( ELISA)检测两组研究对象血清 MCP-1、PGRN、GDNF水平；采用简易智力状态检查表( MMSE)及日常生活量表( ADL)评估其认知功能及日常生活能力。分析观察组病人血清 MCP-1、PGRN、 GDNF水平与 MMSE、ADL评分的相关性；并通过受试者操作特征曲线( ROC曲线)分析 MCP-1、PGRN、GDNF在 AD中的诊断价值。结果观察组 MCP-1(321.7±51.1)ng/L、PGRN(42.6±8.5)μg/L水平较对照组 MCP-1(242.6±35.2)ng/L、PGRN(26.5±6.1)μg/ L显著增高，观察组 GDNF(357.8±112.3)ng/L水平较对照组 GDNF(468.0±106.6)ng/L显著降低，差异有统计学意义( P<0.05)。 Pearson相关分析显示:观察组 MMSE、ADL评分与血清 MCP-1、PGRN水平均呈负相关( r=.0.46~.0.31，P<0.05)与 PGRN水平呈正相关( r=0.47、0.46，P<0.05)。 ROC曲线显示: MCP-1、PGRN、GDNF对 AD的诊断价值较高，其 ROC曲线下面积，(AUC)分别为 0.73、0.75、0.71，灵敏度分别为 75.6％、 75.6％、 70.7％，特异度分别为 61.0％、 70.7％、 63.4％， MCP-1、PGRN、GDNF三项标志物联合检测的 AUC为 0.83，灵敏度和特异度分别达到 82.9％、 75.6％。结论 AD病人血清 MCP-1、PGRN水平表达明显增高， GDNF水平表达明显降低。这与 AD病人认知功能和日常生活能力减退严重程度显著相关。三项指标联合检测在 AD诊断中有着重要的参考价值 . |
| 英文摘要: |
| Objective To analyze the correlation between serum monocyte chemoattractant protein-1 (MCP-1), granule protein pre-cursor (PGRN), glial cell-derived neurotrophic factor (GDNF) and cognitive function and activities of daily living in patients with Al-zheimer's disease (AD), and to explore its diagnostic value in AD.Methods The 41 patients with AD admitted in Guangji Hospital af-filiated to Soochow University from July 2019 to June 2021 were selected as the study group, and 41 healthy volunteers who met theevaluation in the same period were randomly selected as the control group. The levels of serum MCP-1, PGRN and GDNF were mea-sured by enzyme-linked immunosorbent assay (ELISA); The mini mental state examination (MMSE) and daily living scale (ADL)wereused to assess their cognitive function and daily living ability. The correlation between serum MCP-1, PGRN, GDNF levels and MMSEand ADL scores of the study group was analyzed. And through the receiver operating characteristic curve(ROC curve) to analyze the di-agnostic value of MCP-1, PGRN, GDNF in AD.Results TThe levels of MCP-1 (321.7±51.1) ng/L and PGRN (42.6±8.5) μg/L in the study group were significantly higher than those of the control group MCP-1 (242.6±35.2) ng/L and PGRN (26.5±6.1) μg/L , The GDNF(357.8±112.3) ng/L level of the study group was significantly lower than that of the control group GDNF (468.0±106.6) ng/L, and the dif-ference was statistically significant (P<0.05). Pearson correlation analysis showed that MMSE and ADL scores were negatively correlat-ed with serum MCP-1 and PGRN levels in the study group (r= . 0.46-. 0.31, P<0.05). It was positively correlated with the level of PGRN (r=0.47,0.46,P<0.05).The ROC curve showed that MCP-1, PGRN and GDNF had high diagnostic value for AD. the area underthe ROC curve (AUC) was 0.73,0.75,0.71 respectively, the sensitivity was 75.6%, 75.6%,70.7% respectively, and the specificity was61.0%,70.7%,63.4% respectively. The AUC of MCP-1, PGRN and GDNF was 0.83, and the sensitivity and specificity were 82.9%, 75.6% respectively.Conclusions The expression of serum MCP-1 and PGRN in AD patients was significantly increased,The expres-sion of PGRN decreased significantly. This was significantly correlated with the severity of cognitive function and activities of daily liv-ing in AD patients. The combined detection of three indexes has important reference value in the diagnosis of AD. |
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