| 邢志杰,温东朋,郑培明,等.长链非编码 RNA赖氨酰氧化酶样蛋白 1反义 RNA在结直肠癌中的表达及对结直肠癌细胞增殖、侵袭和迁移能力的影响[J].安徽医药,2023,27(5):915-920. |
| 长链非编码 RNA赖氨酰氧化酶样蛋白 1反义 RNA在结直肠癌中的表达及对结直肠癌细胞增殖、侵袭和迁移能力的影响 |
| proliferation, invasion and migration of colorectal cancer cells |
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| DOI：10.3969/j.issn.1009-6469.2023.05.014 |
| 中文关键词: 结直肠肿瘤 长链非编码 RNA 赖氨酰氧化酶样蛋白 1反义 RNA 侵袭 迁移 |
| 英文关键词: Colorectal neoplasms Long non-coding RNA |
| 基金项目:国家自然科学基金资助项目( 81802094) |
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| 摘要点击次数: 621 |
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| 中文摘要: |
| 目的探究长链非编码 RNA(lncRNA)赖氨酰氧化酶样蛋白 1反义 RNA(LOXL1-AS1)在结直肠癌中的表达及对结直肠癌细胞增殖、侵袭及迁移能力的影响和可能的作用机制。方法选取 2016年 1月至 2018年 12月在河南省人民医院行结直肠癌根治术的 56例病人新鲜癌组织，采用实时定量聚合酶链反应( qRT-PCR)检测结直肠癌组织样本及结直肠癌细胞系 SW480、SW620、HCT116和 Lovo细胞中 lncRNA LOXL1-AS1的表达水平，以配对癌旁组织及人正常结直肠黏膜细胞 FHC作正常对照；构建靶向 LOXL1-AS1序列的小干扰 RNA(si-LOXL1-AS1)并转染 SW480和 Lovo细胞，采用细胞计数试剂盒( CCK-8)、平板克隆实验、划痕实验和 Transwell小室法检测沉默 lncRNA LOXL1-AS1表达对结直肠癌细胞增殖、侵袭和迁移能力的影响。采用蛋白质印迹法( Western blotting)检测沉默 LOXL1-AS1表达后磷脂酰肌醇 3-激酶 /蛋白激酶 B(PI3K/Akt)信号通路相关蛋白及上皮 -间质转化( EMT)相关蛋白的表达情况。结果 LOXL1-AS1在结直肠癌组织中的相对表达水平显著高于癌旁组织(1.37±0.05比 0.87±0.05，P<0.05)其表达水平与肿瘤分化程度、 TNM分期、肝转移和 MSI状态显著相关(均 P<0.05)； LOXL1-AS1在结直肠癌细胞系( SW480W620、HCT116、Lovo)中的表达水平显著高于 FHC细胞( 1.93±0.10、1.32±0.04、1.50±0.07、、S，1.78±0.09比 1.10±0.07，P<0.05)沉默 lncRNA LOXL1-AS1表达可抑制 SW480细胞和 Lovo细胞的增殖、侵袭及迁移能力， E-钙黏蛋白( E-cadherin)表达含量升高，，N-钙黏蛋白( N-cadherin)、磷酸化脂酰肌醇 3-激酶( p-PI3K)和磷酸化蛋白激酶 B(p-Akt)表达含量降低(均 P<0.05)。结论 LOXL1-AS1在结直肠癌组织中高表达并与临床病理特征相关，沉默 LOXL1-AS1可显著降低结直肠癌细胞的增殖、侵袭及迁移能力，该机制可能与 PI3K/Akt信号通路及 EMT途径有关。 |
| 英文摘要: |
| Objective To explore the expression of long non-coding RNA (lncRNA) Lysyl Oxidase Like Protein 1 antisense RNA 1 (LOXL1-AS1) in colorectal cancer (CRC), and to investigate the influence of lncRNA LOXL1-AS1 on the proliferation, invasion and mi-gration of CRC cells and the possible mechanism.Methods CRC tissue samples were taken from 56 patients who underwent colorec-tal cancer surgery in Henan Provincial People's Hospital from January 2016 to December 2018. The expression of lncRNA LOXL1-AS1 in 56 CRC tissue samples and CRC cell lines SW480, SW620, HCT116 and Lovo were detected by quantitative real-time PCR (qRT-PCR), and the para-carcinoma tissues and human intestinal epithelial cell line FHC were paired as normal controls. Small inter-fering RNA (si-LOXL1-AS1) targeting the LOXL1-AS1 sequence was constructed and transfected into SW480 and Lovo cells. The ef-fects of silencing lncRNA LOXL1-AS1 expression on the proliferation, invasion and migration of CRC cells were respectively detected by cell counting kit-8 (CCK8), colony formation assays, wound-healing and Transwell assay. Western blotting was used to detect the ex-pression of phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway related proteins and epithelial-mesenchymal transformation (EMT) related proteins.Results The relative expression level of LOXL1-AS1 in CRC tissues was significantly higher than that in adjacent normal tissues (1.37±0.05 vs. 0.87±0.05,P<0.05), which was closely related to tumor differentiation degree, TNM staging, liver metastasis and MSI status of CRC (all P<0.05). The expressions of lncRNA LOXL1-AS1 in CRC cell lines SW480,SW620, HCT116 and Lovo were significantly higher than that of FHC cells (1.93±0.10, 1.32±0.04, 1.50±0.07, 1.78±0.09 vs. 1.10±0.07, P<0.05). The expression of silencing lncRNA LOXL1-AS1 could inhibit the proliferation, invasion and migration of SW480 cells and Lovo cells, up-regulate the expression of E-cadherin, down-regulate the expression of N-cadherin, P-PI3K and p-Akt (all P<0.05).Con. clusions LncRNA LOXL1-AS1 is highly expressed in CRC tissues and is correlated with clinicopathological features of CRC pa-tients. Silencing LOXL1-AS1 can significantly inhibit the proliferation, invasion and metastasis of CRC cells, which may be related to |
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