| 金一鸣,高娜,门学千,等.泛素特异性蛋白酶 2、微 RNA-432-5p在食管癌组织中表达及临床意义[J].安徽医药,2023,27(5):945-949. |
| 泛素特异性蛋白酶 2、微 RNA-432-5p在食管癌组织中表达及临床意义 |
| Expression and clinical significance of ubiquitin-specific protease 2 (USP2) and miR-432-5p in esophageal cancer tissues |
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| DOI：10.3969/j.issn.1009-6469.2023.05.021 |
| 中文关键词: 食管肿瘤 泛素特异性蛋白酶 2 微 RNA-432-5p 临床病理特征 别为， |
| 英文关键词: Esophageal neoplasms Ubiquitin-specific protease 2 miR-432-5p Clinicopathological characteristics |
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| 中文摘要: |
| 目的探讨食管癌组织泛素特异性蛋白酶 2(USP2)、微 RNA-432-5p(miR-432-5p)的表达及临床意义。方法选取 2014年 6月至 2016年 6月在辽宁省肿瘤医院接受外科手术治疗的 93例食管癌病人作为研究对象，取手术切除的食管癌组织及其癌旁组织，采用免疫组织化学法检测 USP2的表达情况，采用实时荧光定量 PCR(qRT-PCR)法检测组织中 USP2 mRNA、 miR-432-5p表达水平，以食管癌组织中 miR-432-5p表达水平平均数分为 miR-432-5p低表达组和 miR-432-5p高表达组，分析食管癌组织 USP2、miR-432-5p表达与食管癌病人临床病理特征的关系；使用生物信息学 Targetscan网站搜索 USP2、miR-432-5p是否存在结合位点，进一步分析食管癌组织 USP2、miR-432-5p的相关性；采用 Kaplan-Meier法分析 USP2、miR-432-5p表达与食管癌病人预后的关系。结果食管癌组织中 USP2 mRNA表达水平( 2.53±0.71比 1.05±0.36)、 USP2阳性率( 55.91%比 6.45%)明显高于癌旁组织( P<0.05)，miR-432-5p表达水平明显低于癌旁组织( 0.31±0.12比 1.03±0.34，P<0.05)；食管癌组织浸润深度(肌层)、 TNM分期( Ⅲ期)、低分化、有淋巴结转移的食管癌组织中 USP2阳性表达率均高于浸润深度(外膜)、 TNM分期( Ⅰ、Ⅱ期)、中高分化、无淋巴结转移的食管癌组织( P<0.05)食管癌组织浸润深度(肌层)、 TNM分期( Ⅲ期)、低分化、有淋巴结转移的食管癌组织中 miR-432-5p表达水平均低于浸润深度(外，膜)、 TNM分期( Ⅰ、Ⅱ期)、中高分化、无淋巴结转移的食管癌组织( P<0.05)；生物信息学 Targetscan网站显示， USP2、miR-432-5p存在结合位点，进一步经 Spearman法分析结果显示食管癌组织中 USP2、miR-432-5p表达呈负相关( P<0.05)Kaplan-Meier结果显示 USP2阳性表达者、 miR-432-5p低表达者 5年总体生存率低于 USP2阴性组者、 miR-432-5p高表达者(分16.032、7.210，P<0.05)。结论 USP2、miR-432-5p异常表达可能与食管癌的发生及预后有关，检测 USP2、miR-432-5p水平对食管癌的早期防治及预后评估具有重要意义，为食管癌的临床治疗提供理论依据。 |
| 英文摘要: |
| Objective To explore the expression and clinical significance of ubiquitin-specific protease 2 (USP2) and miR-432-5p in esophageal cancer tissues.Methods Ninety-three patients with esophageal cancer who underwent surgical treatment in LiaoningCancer Hospital from June 2014 to June 2016 were selected as the research objects, and the surgically removed esophageal cancer tis-sue (as the study group) and its adjacent tissues (as the control group) were taken. Immunohistochemistry was used to detect the expres-sion of USP2, and real-time fluorescent quantitative PCR (qRT-PCR) was used to detect the expression levels of USP2 mRNA and miR-432-5p in the tissues. bBased on the average expression level of miR-432-5p in esophageal cancer tissues, they were assigned into a low expression group of miR-432-5p and a high expression group of miR-432-5p to analyze the relationship between the expressions of USP2 and miR-432-5p in esophageal cancer tissues and the clinicopathological characteristics of patients with esophageal cancer. Thebioinformatics Targetscan website was used to search for the presence of binding sites for USP2 and miR-432-5p to analyze the correla-tion between USP2 and miR-432-5p. The Kaplan-Meier method was used to analyze the relationship between the expressions of USP2, miR-432-5p and the prognosis of patients with esophageal cancer. Results The expression level of USP2 mRNA [(2.53±0.71) vs. (1.05±0.36)] and the positive rate of USP2 (55.91% vs. 6.45%) in esophageal cancer tissue were significantly higher than those in adja-cent tissues P<0.05), and the expression level of miR-432-5p [(0.31±0.12) vs. (1.03±0.34)] was significantly lower than those in adja-cent tissues (P<0.05); the positive expression rates of USP2 in esophageal cancer tissues with infiltration depth to muscular layer, TNMstaging (stage Ⅲ), poor differentiation, and lymph node metastasis were higher than those in esophageal cancer tissues with infiltrationdepth to adventitia, TNM staging (stage Ⅰ,Ⅱ), medium and high differentiation, and non-lymph node metastasis (P<0.05). The positive expression rates of miR-432-5p in esophageal cancer tissues with infiltration depth to muscular layer, TNM staging (stage Ⅲ), poor dif-ferentiation, and lymph node metastasis were lower than those in esophageal cancer tissues with infiltration depth to adventitia, TNMstaging (stage Ⅰ , Ⅱ), medium and high differentiation, and non-lymph node metastasis (P<0.05); bioinformatics Targetscan website showed that there were binding sites for USP2 and miR-432-5p, and further analysis by Spearman method showed that the expressions of USP2 and miR-432-5p in esophageal cancer tissue were negatively correlated (P<0.05). Kaplan Meier analysis results showed that the 5-year overall survival rates of USP2 positive group and miR-432-5p low expression group were lower than those of USP2 negative group and miR-432-5p high expression group (16.032 and 7.210, respectively, P<0.05). Conclusion The abnormal expressions of USP2 and miR-432-5p may be related to the occurrence and prognosis of esophageal cancer. The detection of USP2 and miR-432-5plevels is of great significance for the early prevention and prognosis evaluation of esophageal cancer, which provides a theoretical basisfor the clinical treatment of esophageal cancer. |
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