| 张静,杨晔,董超,等.计算机断层扫描联合血清三叶因子 1、微 RNA-130a-5p检测对肺癌的诊断价值分析[J].安徽医药,2023,27(5):989-992. |
| 计算机断层扫描联合血清三叶因子 1、微 RNA-130a-5p检测对肺癌的诊断价值分析 |
| The diagnostic value of CT combined with serum TFF1 and miR-130a-5p detection in lung cancer |
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| DOI：10.3969/j.issn.1009-6469.2023.05.031 |
| 中文关键词: 肺肿瘤 计算机断层扫描 血清三叶因子 1 微 RNA-130a-5p |
| 英文关键词: Lung neoplasms Computed tomography Serum trefoil factor 1 miR-130a-5p |
| 基金项目:河北省卫生健康委员会资助项目( 20191462) |
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| 中文摘要: |
| 目的探究计算机断层扫描(CT)联合血清三叶因子 1(TFF1)、微 RNA-130a-5p(miR-130a-5p)检测对肺癌的诊断价值。方法选取 2015年 2月至 2016年 9月石家庄市人民医院收治的 94例肺癌病人为肺癌组， 85例同期确诊的肺良性病变病人为良性病变组。对所有受试者进行 CT检查，并分析 CT对肺癌病人的诊断效能；采用酶联免疫吸附法( ELISA)检测血清 TFF1水平；采用实时荧光定量 PCR(qRT-PCR)检测血清 miR-130a-5p相对表达量； ROC曲线分析血清 TFF1、miR-130a-5p对肺癌的诊断价值；分析 CT、TFF1、miR-130a-5p单独检测及联合检测对肺癌的诊断价值。结果 CT诊断肺癌误诊 16例， 19例漏诊。 CT诊断肺癌的灵敏度为 79.79%，特异度为 81.18%，准确度为 80.45%，误诊率、漏诊率分别为 18.82%与 20.21%。与良性病变组相比，肺癌组病人血清 TFF1显著上升， miR-130a-5p水平显著下降( P<0.05)。血清 TFF1、miR-130a-5p对肺癌诊断价值的曲线下面积( AUC)分别为 0.85、0.83，截断值分别为 1.17 μg/L、0.72。与联合检测相比， CT检测的灵敏度、准确度显著降低，漏诊率显著升高；血清 miR-130a-5p检测的特异度、准确度均显著降低，误诊率显著升高( P<0.05)。结论肺癌病人血清 TFF1、miR-130a-5p呈异常表达， CT联合血清 TFF1、miR-130a-5p可提高肺癌诊断的灵敏度和特异度，可为肺癌的诊治提供参考依据。 |
| 英文摘要: |
| Objective To explore the value of computed tomography (CT) combined with serum trefoil factor 1 (TFF1) and miR-130a-5p in the diagnosis of lung cancer.Methods A total of 94 lung cancer patients admitted to Shijiazhuang People's Hospital fromFebruary 2015 to September 2016 were selected as the lung cancer group, another 85 patients with benign lung lesions were selected asthe benign lesion group. CT examinations were performed on all subjects and the diagnostic efficacy of CT for lung cancer patients wasanalyzed. Enzyme-linked immunosorbent assay (ELISA) was used to check the level of serum TFF1 in patients, and real-time fluores-cence quantification PCR (qRT-PCR) was used to detect the relative expression level of serum miR-130a-5p in patients. ROC curve |
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