| Objective To establish a high performance liquid chromatography-diode array detector (HPLC-DAD) method for simultaneous determination of 7 effective components in Gengnianning.Methods From June 2021 to February 2022, the chromatographic separation of HPLC-DAD method was achieved on a C18 column (4.6 mm×250 mm, 5 μm) with 0.1 % phosphoric acid solution-acetonitrile as mobile phase at the flow rate of 1.0 mL/min for gradient elution; The column temperature was 35 ℃, and the detection wavelengthswere 232 nm, 226 nm and 276 nm.Results The linear relationships of paeoniflorin, specnuezhenide, baicalin, wogonoside, paeony phenol, baicalein, wogonin were good within the ranges of 6.24-103.96, 8.21-136.80, 14.48-241.36, 2.29-38.10, 3.33-55.46, 1.16-19.36, 0.43-7.22 mg/L, respectively. The correlation coefficient was not less than 0.999 5, and the average recoveries were 100.00%, 99.30%,98.48%, 97.26%, 97.94%, 102.01% and 99.33%, respectively, with the RSDs ranging from 2.09% to 4.05%.Conclusion The method is accurate, reliable and sensitive. It provides technical reference for further improving quality standard of Gengnianning. |
