| Objective To develop a method for the simultaneous determining the content of six flavonoids in Zhixue Huazhi mixture by a quantitative analysis of multi-components with single marker (QAMS). Methods Baicalin was taken as internal standard sub stance to establish the relative correction factor (f) of rutin, liquiritin, quercetin, baicalein, wogonin by HPLC. The contents of above six flavonoids were calculated through f. Meanwhile, external standard method (ESM) was used to calculate the content of six flavonoids.The difference of two methods were compared to validate the accuracy and feasibility of QAMS.Results The standard curves of rutin,liquiritin, baicalin, quercetin, baicalein, wogonin had good linear relationship in ranges of the tested concentrations (r>0.997). The precision, stability and repeatability complied with the requirements of methodology. The average recoveries were 95.72%-100.43%, and the RSD were 1.97%-3.22%. The relative correction factors of rutin, liquiritin, quercetin, baicalein, and wogonin were 6.426, 3.939,3.215, 41.140, 0.555, with good repeatability. There was no significant difference of the quantitative results between QAMS and ESMmethod (P>0.05).Conclusions The QAMS established with baicalin as internal standard substance simultaneously determine the con tent of six flavonoids in Zhixue Huazhi mixture. The method is simple, accurate and reliable, and can be used for the quality evaluation of Zhixue Huazhi mixture. |
