| 卢壮,刘敏,韦艳梅,等.电针调控肿瘤坏死因子受体 1/受体相互作用蛋白激酶 1/核因子 κB轴对骶髓损伤后神经源性膀胱尿潴留大鼠尿动力学的影响[J].安徽医药,2023,27(8):1655-1660. |
| 电针调控肿瘤坏死因子受体 1/受体相互作用蛋白激酶 1/核因子 κB轴对骶髓损伤后神经源性膀胱尿潴留大鼠尿动力学的影响 |
| Electroacupuncture improves urodynamics in rats with neurogenic bladder urinary retention after sacral cord injury by regulating TNFR1/RIPK1/NF-κB axis |
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| DOI：10.3969/j.issn.1009-6469.2023.08.036 |
| 中文关键词: 脊髓损伤 腰骶丛 电针 神经源性膀胱 尿动力学 炎症 |
| 英文关键词: Spinal cord injuries Lumbosacral plexus Electroacupuncture Neurogenic bladder Urodynamics Inflammation |
| 基金项目:百色市科学研究与技术开发计划(百科 20184408) |
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| 中文摘要: |
| 目的探究电针调控肿瘤坏死因子受体 1/受体相互作用蛋白激酶 1/核因子 κB(TNFR1/RIPK1/NF-κB)轴对骶髓损伤(SCI)后神经源性膀胱( NB)尿潴留大鼠的影响及机制。方法采用随机数字表法将 SD大鼠分为空白组、模型组、电针组和电电针组取穴“中极”针对照组，每组 10只。建立 SCI后 NB尿潴留模型，“次髎”“三阴交”电针对照组取三穴对照点，行电针治疗 7d。各组大鼠治疗前、后行尿动力学检测；取大鼠脊髓组织，实时荧光定量逆转录聚，合酶链反应( qRT-PCR)检测 TNFR1、 RIPK1表达；蛋白质印迹法( Western blotting)检测 TNFR1、RIPK1、p-IKKβ/IKKβ、p-NF-κB p65/NF-κB p65和 p-IκBα/IκBα蛋白表达；酶联免疫吸附剂测定法( ELISA)检测 TNF-α、IL-1β、IL-6、IFN-γ含量。该研究起止时间为 2019年 1月至 2021年 12月。结果与空白组比较，模型组大鼠膀胱最大容量［(4.46±0.82)mL比( 1.13±0.22)mL，P<0.05］、膀胱基础压［(32.00±4.23)cmH2O比( 22.97±3.71)cmH2O，P<0.05］、漏尿点压力［( 54.02±7.61)cmH2O比( 38.85±5.32)cmH2O，P<0.05］和膀胱顺应性［( 0.19±0.03) mL/cmH2O比( 0.08±0.01)mL/cmH2O，P<0.05］升高， TNFR1、RIPK1、p-IKKβ/IKKβ、p-NF-κB p65/NF-κB p65、p-IκBα/IκBα表达及 TNF-α、IL-1β、IL-6、IFN-γ含量增加( P<0.05)；与模型组比较，电针组治疗后大鼠膀胱最大容量［(2.29±0.43)mL比( 4.46±0.82) mL，P<0.05］、膀胱基础压［( 23.69±3.43)cmH2O比( 32.00±4.23)cmH2O，P<0.05］、漏尿点压力［( 40.11±5.91)cmH2O比( 54.02±7.61)cmH2O，P<0.05］和膀胱顺应性［( 0.12±0.02)mL/cmH2O比( 0.19±0.03)mL/cmH2O，P<0.05］降低， TNFR1、RIPK1、p-IKKβ/ IKKβ、p-NF-κB p65/NF-κB p65、p-IκBα/IκBα表达及 TNF-α、IL-1β、IL-6、IFN-γ含量减少( P<0.05)；电针对照组与模型组比较差异无统计学意义( P>0.05)。结论电针“次髎”“中极”“三阴交”可改善 SCI后 NB尿潴留大鼠膀胱功能，机制与抑制 TNFR1/ RIPK1/NF-κB轴活化，抑制炎症反应有关。 |
| 英文摘要: |
| Objective To explore the effect and mechanism of electroacupuncture on rat with neurogenic bladder (NB) urinary retention after sacral cord injury (SCI) by regulating the tumor necrosis factor receptor 1/receptor-interacting protein kinase 1/nuclear factor kappa-B (TNFR1/RIPK1/NF-κB) axis.Methods The SD rats were assigned into blank group, model group, electroacupuncture groupand electroacupuncture control group, with 10 rats in each group. A model of NB urine retention after SCI was established. The electroacupuncture group took the points of "Ciliao (BL32)""Zhongji (RN3)", and "Sanyinjiao (SP6)", and the control group took the threeacupoint control points and treated them with electroacupuncture for 7 days. Urodynamic tests were performed before and after treatment in each group of rats. The rat spinal cord tissue was collected, and the expression of TNFR1 and RIPK1 was detected by qRT-PCR, the expression of TNFR1,RIPK1,p-IKKβ/IKKβ, p-NF-κB p65/NF-κB p65 and p-IκBα/IκBα was detected by Western Blotting, the content of TNF-α, IL-1β, IL-6, IFN-γ was detected by Enzyme-linked immunosorbent assay (ELISA). The study began and ended from January 2019 to December 2021. Results Compared with the blank group, the maximum bladder capacity [(4.46±0.82)mL vs. (1.13±0.22)mL,P<0.05], basal bladder pressure [(32.00±4.23)cmH2O vs. (22.97±3.71)cmH2O,P<0.05], leakage point pressure [(54.02± 7.61)cmH2O vs. (38.85±5.32)cmH2O, P<0.05] and bladder compliance [(0.19±0.03)mL/cmH2O vs. (0.08±0.01)mL/cmH2O, P<0.05] in creased in the model group, the expression of TNFR1, RIPK1, p-IKKβ/IKKβ, p-NF-κB p65/NF-κB p65, p-IκBα/IκBα and the content of TNF-α, IL-1β, IL-6 and IFN-γ increased, and the difference was statistically significant (P<0.05). Compared with the model group, the maximum bladder capacity [(2.29±0.43)mL vs. (4.46±0.82)mL, P<0.05], basal bladder pressure [(23.69±3.43)cmH2O vs. (32.00± 4.23)cmH2O, P<0.05], leakage point pressure [(40.11±5.91)cmH2O vs. (54.02±7.61)cmH2O, P<0.05] and bladder compliance [(0.12±0.02)mL/cmH2O vs. (0.19±0.03)mL/cmH2O,P<0.05] of rats in the electroacupuncture group decreased after treatment, the expression of TNFR1, RIPK1, p-IKKβ/IKKβ, p-NF-κB p65/NF-κB p65, p-IκBα/IκBα and the content of TNF-α, IL-1β, IL-6, IFN-γ decreased (P< 0.05). There was no significant difference between the electroacupuncture control group and the model group (P>0.05). Conclusion Electroacupuncture of "Ciliao (BL32)""Zhongji (RN3)",and "Sanyinjiao (SP6)" can improve the bladder function of NB urinary retention rats after SCI. The mechanism is related to inhibiting the activation of TNFR1/RIPK1/NF-κB pathway and inhibiting inflammation. |
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