| 张子杭,成元华.转化生长因子 -β通路对胃癌细胞在裸鼠体内增殖、侵袭及 slug蛋白和上皮钙黏素基因表达的影响[J].安徽医药,2023,27(9):1804-1808. |
| 转化生长因子 -β通路对胃癌细胞在裸鼠体内增殖、侵袭及 slug蛋白和上皮钙黏素基因表达的影响 |
| Effect of transforming growth factor-β pathway on proliferation, invasion and expressions of slug protein and epithelial cadherin genes in gastric cancer cells of nude mice |
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| DOI：10.3969/j.issn.1009-6469.2023.09.024 |
| 中文关键词: 胃肿瘤 转化生长因子 -β 信号通路 上皮钙黏素 slug 小鼠，近交 BALB C |
| 英文关键词: Stomach neoplasms TGF-β Signal pathway E-cadherin Slug Mice, inbred BALB C |
| 基金项目:贵州省科技计划项目(黔合科 J字［ 2009］2187号) |
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| 中文摘要: |
| 目的研究转化生长因子 -β(TGF-β)通路的激活与阻断对人胃癌 HGC-27细胞增殖、侵袭及 slug蛋白和上皮钙黏素(E-cad)基因表达的影响。方法于 2021年 4月至 2022年 3月使用人胃癌 HGC-27细胞构建 30只胃癌细胞裸鼠皮下移植瘤模型后，使用外源性通路激活剂 TGF-β1和阻断剂 SB431542对 TGF-β通路进行激活和阻断。使用随机数字表法将所有裸鼠分为五组( n=6)。空白对照组:注射等量生理盐水，激动剂组:皮下注射 TGF-β1(1微克 /只)，阻断剂组:腹腔注射 SB431542(0.7 mg/kg)，激动剂 +低浓度阻断剂组: TGF-β1(1微克 /只) +SB431542(0.7 mg/kg)，激动剂 +高浓度阻断剂组: TGF-β1(1微克 /只) +SB431542(1.0 mg/kg)。饲养至 24 d，统一处死并记录不同组裸鼠的肿瘤质量与体积，采用逆转录 -聚合酶链反应( RTPCR)与蛋白质印迹法检测肿瘤组织中 slug与 E-cad基因的表达。结果使用通路激活剂激活通路后，胃癌移植瘤体积 |
| 英文摘要: |
| Objective To explore the effects of activation and blocking of transforming growth factor-β (TGF-β) pathway on the pro- liferation and invasion of human gastric cancer HGC-27 cells and the expressions of slug protein and epithelial cadherin (E-cad) gene. Methods From April 2021 to March 2022, human gastric cancer HGC-27 cells were used to construct a subcutaneous tumor model of 30 nude mice with gastric cancer cells, and the exogenous pathway activator TGF-β1 and blocker SB431542 were used to activate and block the TGF-β pathway. All mice were assigned into 5 groups (n=6) by random number table method. The treatment methods of eachgroup were as follows: blank control group was injected with the same amount of normal saline, agonist group injected with TGF-β1 sub- cutaneously (1 μg/mice), blocker groupinjected with SB431542 intraperitoneally (0.7 mg/kg), agonist + low-concentration blocker group with TGF-β1 (1 μg/mice) + SB431542 (0.7 mg/kg), and agonist + high-concentration blocker group with TGF-β1 (1 μg/mice) +SB431542 (1.0 mg/kg). After feeding for 24 days, the mice were killed and the tumor sizes and mass of nude mice in different groupswere recorded. The expressions of slug and E-cad genes in tumor tissue were detected by reverse transcription-polymerase chain reac- tion (RT-PCR) and Western blotting (WB).Results After activating the pathway with a pathway activator, the tumor size [(854.00± 193.00) mm3 vs. (544.00±60.00) mm3], the mass [(2.74±0.52)g vs. (1.52±0.41) g], and the expression level of slug protein [(2.13±0.08) vs. (1.00±0.00)] were higher than those of the control group. The expression level of E-cad gene in the agonist group [(0.44±0.04) vs. (1.00±0.00)] was lower than that in the control group (P<0.05).Conclusion TGF-β pathway can enhance the expression of slug pro- tein and inhibit the expression of E-cad, activate epithelial-mesenchymal transition (EMT), and improve the proliferation and invasionof gastric cancer cells in vivo, thereby affecting the progression of gastric cancer. |
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