| 含笑,胡茂能,王国亮,等.基于数字减影血管造影的 VX2肝癌兔靶向药物治疗模型的建立[J].安徽医药,2023,27(9):1824-1827. |
| 基于数字减影血管造影的 VX2肝癌兔靶向药物治疗模型的建立 |
| Modeling of targeted drug therapy for VX2 hepatocellular carcinoma in rabbit based on digital subtraction angiography |
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| DOI：10.3969/j.issn.1009-6469.2023.09.028 |
| 中文关键词: 肝肿瘤 疾病模型，动物 贝伐单抗 化学栓塞，治疗性 数字减影血管造影 兔 VX2肝癌 |
| 英文关键词: Liver neoplasms Disease models, animal Bevacizumab Chemoembolization, therapeutic Digital subtraction an- giography Rabbit VX2 hepatocellular carcinoma |
| 基金项目:合肥市卫健委 2019年应用医学研究项目(重点项目 14号)；合肥市第六周期医学重点学科建设基金(合卫科教〔 2019〕160号) |
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| 中文摘要: |
| 目的探讨基于数字减影血管造影( DSA)的 VX2兔肝癌模型建立以及影响建模成功的相关因素。方法 2019年 11月至 2022年 6月， 44只健康新西兰大白兔使用随机数字表法等分成 A、B两组，各 22只。均实施开腹穿刺接种法移植肝癌， A组行常规经导管动脉化疗栓塞术( TACE)手术治疗(阿霉素 +碘化油)B组在传统 TACE治疗的基础上联合使用靶向药物贝伐单抗。根据手术后生存时间分为死亡组(存活时间 <14 d)和存活组(存活，时间 ≥14 d)将可能影响存活的因素分别行单因素及 lo- gistic多因素回归分析，使用受试者操作特征曲线( ROC曲线)分析相关因素的诊断预，测效能。结果死亡组为 10只，存活组为 34只。多因素分析显示体质量［ OR=0.20，95%CI:(0.07，0.59)］、总胆红素［ OR=6.02，95%CI:(2.55，14.16)］是 VX2肝癌兔术后短期死亡的独立影响因素。 ROC曲线分析显示当肝癌兔体质量 ≤2.44 kg、总胆红素 ≥4.84 μmol/L时预测术后短期死亡的效能最高，此时曲线下面积( AUC)分别为 0.90和 0.89。结论基于 DSA的 VX2肝癌兔靶向药物治疗模型的建立成功率较为可靠，但较低的体质量和较高的术后血清总胆红素是肝癌兔术后短期内死亡的独立危险因素。 |
| 英文摘要: |
| Objective To investigate the establishment of a VX2 rabbit hepatocellular carcinoma model based on digital subtractionangiography (DSA) and the related factors affecting the success of modeling.Methods From November 2019 to June 2022, 44 healthyNew Zealand White rabbits were equally divided into two groups, A and B, of 22 rabbits each, using the random number table method.All subjects underwent open abdominal puncture inoculation for transplantation of hepatocellular carcinoma. Group A underwent con-ventional transcatheter arterial chemoembolization (TACE) surgery (adriamycin + iodized oil), and group B was combined with the tar-geted drug bevacizumab based on conventional TACE treatment. According to the survival time after surgery, the rabbits were dividedinto the death group (survival time < 14 d) and the survival group (survival time ≥ 14 d), and the factors that might affect survival wereanalyzed by univariate and logistic multivariate regression analysis. The diagnostic predictive efficacy of the relevant factors was ana-lyzed using receiver operating characteristic (ROC) curves.Results There were 10 animals in the death group and 34 animals in the survival group. Multivariate analysis revealed that body weight [OR=0.20, 95% CI: (0.07, 0.59)]and total bilirubin (TB) [OR=6.02, 95%CI: (2.55, 14.16)] were independent influencing factors for short-term postoperative mortality in VX2 hepatic carcinoma rabbits. ROC curve analysis showed the highest efficacy in predicting short-term postoperative mortality in hepatocellular carcinoma rabbits whentheir body mass was ≤ 2.44 kg and total bilirubin was ≥ 4.84 μmol/L, at which time the area under the curve (AUC) was 0.90 and 0.89,respectively.Conclusion The success rate of DSA-based modeling of targeted drug therapy in rabbits with VX2 hepatocellular carcino-ma was more reliable, but lower body mass and higher postoperative serum TB were independent risk factors for short-term postopera- tive death in rabbits with hepatocellular carcinoma. |
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