| 崔桂荣,张金华,刘亚宁,等.微 RNA-194在宫颈癌中的表达及临床意义[J].安徽医药,2023,27(9):1857-1860. |
| 微 RNA-194在宫颈癌中的表达及临床意义 |
| Expression and clinical significance of miRNA-194 in cervical cancer |
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| DOI：10.3969/j.issn.1009-6469.2023.09.036 |
| 中文关键词: 子宫肿瘤 子宫颈 微 RNA-194 实时荧光定量逆转录聚合酶链反应 预后 |
| 英文关键词: Uterine neoplasms Cervix uteri MiRNA-194 Quantitative real time polymerase chain reaction Prognosis |
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| 中文摘要: |
| 目的探讨微 RNA(miRNA)-194在宫颈癌组织中的表达情况及其临床意义。方法回顾性选取 2013年 5月至 2016年 1月于邯郸市第一医院行手术治疗的 136例宫颈癌病人为研究对象，采用分层抽样选取 58例宫颈癌病人的癌组织及相应癌旁正常组织，其余 78例病人仅选取癌组织，获取 136例宫颈癌组织标本及 58例癌旁正常组织标本。采用实时荧光定量逆转录聚合酶链反应( qRT-PCR)检测宫颈癌组织和癌旁组织中 miRNA-194表达水平，分析 miRNA-194表达与宫颈癌病人临床病理特征及预后的关系。结果 qRT-PCR结果表明 136例宫颈癌病人癌组织 miRNA-194的表达量( 1.140±0.655)明显低于癌旁组织( 4.502±1.168)(P<0.001)。 miRNA-194表达水平与宫颈癌病人的国际妇产科联盟( FIGO)分期( P=0.001)和肿瘤长径( P=0.033)相关。 miRNA-194低表达的宫颈癌病人的生存期显著低于 miRNA-194高表达病人［ HR 95%CI=2.17(1.21，3.90)， log-rank P=0.012］。 Cox回归分析结果表明 miRNA-194表达是影响宫颈癌病人预后的独立因素。结论 miRNA-194在宫颈癌的发生发展中有一定作用，其在宫颈癌病人癌组织中更低， miRNA-194有可能成为宫颈癌诊断和预后的指标。 |
| 英文摘要: |
| Objective To detect the microRNA (miRNA)-194 expression level in cervical cancer, and to explore its clinical signifi- cance.Methods Totally 136 cervical cancer patients, who underwent surgery in The First Hospital of Handan City from May 2013 toJanuary 2016, were retrospectively selected as research objects. Cancer tissue and corresponding adjacent normal cervical tissue speci-mens of 58 patients were collected by stratified sampling method and only cancer tissue were collected from the other 78 patients. Total-ly there were 136 specimens of cervical cancer tissue and 58 specimens of adjacent normal tissue. The expressions of miRNA-194 in cancer tissue and adjacent tissue were detected by quantitative real time polymerase chain reaction (qRT-PCR), and its relationship with clinicopathological features and prognosis of cervical cancer patients was analyzed.Results qRT-PCR results showed that the ex- pression level of miRNA-194 in cancer tissue of 136 patients with cervical cancer was significantly lower than that in adjacent tissue [(1.140±0.655) vs. (4.502±1.168), P<0.001]. The expression level of miRNA-194 in cervical cancer was associated with FIGO (Interna- tional Federation of Gynecology and Obstetrics) stage (P=0.001) and tumor size (P=0.033). Overall survival of cervical cancer patients with lower levels of miRNA-194 was significantly shorter than those with higher levels of miRNA-194 [HR 95%CI=2.17 (1.21, 3.90), log-rank P=0.012].Cox regression analysis results showed that miRNA-194 expression was an independent risk factor for prognosis of cervical cancer patients.Conclusion miRNA-194 plays a certain role in the occurrence and development of cervical cancer, the ex-pression of which is lower in the cancer tissue of cervical cancer patients, and may serve as a potential diagnostic and prognostic mark-er for cervical cancer. |
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