| 王树根,宁美英,王颖,等.知母山楂饮对大鼠非酒精性脂肪肝的治疗作用及可能机制的研究[J].安徽医药,2023,27(10):1927-1932. |
| 知母山楂饮对大鼠非酒精性脂肪肝的治疗作用及可能机制的研究 |
| Study on the therapeutic effect and mechanism of anemarrhenae-hawthorn drink on non-alcoholic fatty liver in rats |
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| DOI：10.3969/j.issn.1009-6469.2023.10.005 |
| 中文关键词: 脂肪肝 知母山楂饮 大鼠 高脂血症 1β-羟基类固醇脱氢酶 1(11β-HSD1) 髓样分化因子初次应答基因 88(myd88) Toll样受体 4(TLR4) 核因子 κB(NF-κB) |
| 英文关键词: Fatty liver Anemarrhenae-hawthorn drink Rat Hyperlipidemias 11β-Hydroxysteroid dehydrogenase 1 Medullary differentiation factor first response gene 88 Toll like receptor 4 NF-kappa B |
| 基金项目:河北省中医药管理局中医药类科研计划项目( 2022615) |
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| 中文摘要: |
| 目的研究知母山楂饮对非酒精性脂肪性肝病( NAFLD)大鼠的治疗作用。方法 2021年 6—12月，在沧州医学高等专科学校实验中心将 55只雄性 SD大鼠(其中 5只大鼠用于判断是否造模成功)经过适应性喂养后，按照随机数字表法分为正常组(基础饲料喂养)、模型组(高脂饲料喂养进行 NAFLD模型造模)、阳性对照组(造模成功后给予普罗布考 500 mg/kg)、低剂量给药组(造模成功后给予知母山楂饮 2.5 g/kg)、高剂量给药组(造模成功后给予知母山楂饮 5 g/kg)，每组 10只。造模后培养 8周，对各组大鼠称体质量，同时进行胰岛素耐量试验，以及测定各组大鼠血清中总胆固醇( TC)三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、谷草转氨酶(AST)、谷丙转氨酶(ALT)、超氧、化物歧化酶(SOD)、丙二醛(MDA)。之后，各组的大鼠被处死，剖取各组大鼠肝脏组织。计算肝脏指数后，经 HE染色、油红 O染色后观察组织病理形态学变化。并测定肝脏组织的 SOD、MDA、肿瘤坏死因子 α(TNF-α)和白细胞介素 -6(IL-6)。对肝脏组织 11β-羟基类固醇脱氢酶 1(11β-HSD1)、髓样分化因子初次应答基因 88(myd88)、 Toll样受体 4(TLR4)和核因子 κB(NF-κB)mRNA表达及蛋白表达检测。结果低剂量给药组大鼠体质量( 515.72±20.55)g明显低于模型组( 595.26±27.88)g(P<0.01)其中低剂量给药组肝脏指数变化( 2.65±0.05)%明显低于模型组( 3.05±0.09)%(P<0.01)；累积糖耐量低剂量给药组( 14.63±1.05)，mmol·L-1 ·h-1明显低于模型L-1h-1组( 17.95±1.09)mmol··(P<0.05)可改善胰岛素抵抗；很好改善大鼠血清中 TC、TG、LDL-C、AST、ALT、MDA水平，升高HDL-C、SOD水平(P<0.05)；降低肝脏组织，组织中 11β-HSDl、MyD88、TLR4B和 NF-κB表达水平( P<0.05)；并且高剂量给药组效果明显。结论知母山楂饮可以很好地改善高脂饲料引发的大鼠非酒精性肝损伤。 |
| 英文摘要: |
| Objective To investigate the therapeutic effect of rhizoma anemarrhenae hawthorn decoction on nonalcoholic fatty liver in rats. Methods From June to December 2021, 55 male SD rats (5 of which were used to judge the success of modeling) were fed inthe Experimental Center of Cangzhou Medical College. After adaptive feeding, rats were divided into normal group (fed with basal diet),model group (fed with high-fat diet for nonalcoholic fatty liver model), positive control group (treated with probucol 500 mg/kg), lowdose group (treated with rhizoma anemarrhenae hawthorn decoction 2.5 g/kg) and high dose group (treated with rhizoma anemarrhenaehawthorn decoction 5 g/kg). The rats were weighed after 8 weeks of feeding. At the same time, insulin tolerance test and serum total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), aspartate aminotransferase (AST), glutamic pyruvic transaminase (ALT), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured.Thereafter, the rats in each group were sacrificed and the liver tissues of each group were dissected. After calculating the liver index,the histopathological changes were observed after HE staining and Oil red O staining. And the SOD, MDA, TNF-α and IL-6 in the liver were measured. The mRNA and protein expression of 11b-HSDl, MyD88, TLR4B and NF-kB were detected in liver tissue. Results Compared with the model group, the positive control group and each administration group could slow down the growth of body mass ofrats, and that of the low dose administration group (515.72±20.55) g was significantly lower than that of the model group (595.26±27.88) g, (P < 0.01) and liver index changes. The low dose group (2.65±0.05) % was significantly lower than the model group (3.05± 0.09) % (P< 0.01); Insulin resistance was improved, and the cumulative glucose tolerance in low-dose administration group (14.63± 1.05) mmol·L-1·h-1 was significantly lower than that in model group (17.95±1.09) mmol·L-1·h-1(P < 0.05). The levels of TC, TG, LDLC, AST, ALT and MDA in serum were improved, and the levels of HDL-C and SOD were increased (P < 0.05). The pathological changes of liver tissue were improved. The expression levels of 11β-HSDl, MyD88, TLR4B and NF-κB in liver tissues were decreased (P < 0.05). And the effect of high dose administration group was obvious. Conclusion Rhizoma anemarrhenae hawthorn decoction can improve nonalcoholic liver injury induced by high fat diet in rats. |
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