| 赵向阳,余秀萍,吴玮,等.甜叶菊药材品质与质量提升研究[J].安徽医药,2023,27(10):1937-1940. |
| 甜叶菊药材品质与质量提升研究 |
| Research on improvement of the qualities of Stevia rebaudiana (bertoni) hemsl. |
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| DOI：10.3969/j.issn.1009-6469.2023.10.007 |
| 中文关键词: 甜菊属 主成分分析 薄层色谱 高效液相色谱(HPLC) 甜菊苷 莱苞迪苷 A |
| 英文关键词: Stevia Principal component analysis Thin-layer chromatography(TLC) High-performance liquid chromatography (HPLC) Stevioside Rabaudisode A |
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| 中文摘要: |
| 目的建立甜叶菊的质量标准研究方法。方法在 2020年 10月至 2021年 12月，参照 2020年版《中国药典(四部)，对药材样品的显微特征、水分、总灰分、酸不溶性灰分、醇溶性浸出物进行检测；采用薄层色谱( TLC)法对药材样品进行定性鉴》别；采用高效液相色谱( HPLC)法测定药材样品中甜菊苷和莱苞迪苷 A的含量，色谱柱为 Shim-pack GIST(4.6 mm×250 mm，5 μm)，流动相为乙腈 -水( 35∶65)，流速为 0.5 mL/min，检测波长为 210 nm，柱温为 40 ℃，进样量为 10 μL。结果药材样品粉末呈暗绿色或黄绿色，显微特征清晰。 TLC图斑点清晰，且分离度好。甜菊苷和莱苞迪苷 A分别在 0.10～2.01 g/L(r=0.999 7)和 |
| 英文摘要: |
| Objective To establish a method for the study of quality standards of Stevia rebaudiana (bertoni) hemsl.Methods Between October 2020 and 2021 December, the microscopic characteristics, moisture content, total ash, acid-insoluble ash, and alcohol-soluble extracts of the Stevia rebaudiana (Bertoni) Hemsl. were determined according to the Chinese Pharmacopoeia (2020 Edition, Volume Ⅳ). Thin-layer chromatography (TLC) was used to qualitatively identify Stevia rebaudiana (bertoni) hemsl. High-performance liquid chromatography (HPLC) method was used to determine the content of stevioside and rebaudioside A in Stevia rebaudiana (bertoni) hemsl. The chromatographic column was Shim-pack GIST column(4.6 mm×250 mm,5 μm), the mobile phase was acetonitrile-water(35∶ 65, V/V), the flow rate was 0.5 mL/min, the detection wavelength was 210 nm, the column temperature was 40 ℃, and the injection volume was 10 μL.Results The sample powder was dark green or chartreuse, with clear microscopic characteristics. The TLC spots wereclear with good separation. The linear ranges of stevioside and rebaudioside A were 0.10-2.01 g/L (r=0.999 7) and 0.10-1.17 g/L (r=0.999 9), respectively. The RSDs of precision, stability and repeatability tests were less than 2.00%. The average recoveries of stevioside and rebaudioside A were 98.5% and 98.4%, with RSDs of 0.30% and 0.45%(n=6), respectively. The average content of moisture, total ash and acid-insoluble ash of the ten batches of samples was 4.0%, 7.2% and 0.9%, respectively, and their alcohol-soluble extract contents were more than 50%. The average content of stevioside and rebaudioside A was 11.4% and 4.1%, respectively.Conclusion The established standard can be used for the quality control of decoction slices of Stevia rebaudiana (bertoni) hemsl. |
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