| 王世信,杨美娜,崔友祥,等.超高效液相色谱波长切换法同时测定芪参还五胶囊中 4种成分含量[J].安徽医药,2023,27(10):1941-1944. |
| 超高效液相色谱波长切换法同时测定芪参还五胶囊中 4种成分含量 |
| Simultaneous determination of four components in Qishen Huanwu capsules by UPLC with wavelength switching method |
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| DOI：10.3969/j.issn.1009-6469.2023.10.008 |
| 中文关键词: 主成分分析 色谱法，高压液相 芪参还五胶囊 羟基红花黄色素 A 芍药苷 阿魏酸 毛蕊异黄酮葡萄糖苷 |
| 英文关键词: Principal component analysis Chromatography, high pressure liquid Qishen Huanwu capsules Hydroxysafflor yellow A Paeoniflorin Ferulic acid Calycosin-7-glucoside |
| 基金项目:河北省中医药管理局科研项目( 2019267) |
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| 摘要点击次数: 603 |
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| 中文摘要: |
| 目的建立超高效液相色谱(UPLC)波长切换法同时测定芪参还五胶囊中羟基红花黄色素 A、芍药苷、阿魏酸和毛蕊异黄酮葡萄糖苷 4种化学成分含量。方法 2021年 5月至 2022年 1月，采用 UPLC，色谱柱为 WATERS ACQUITY UPLC BEH C18(2.1 mm×50 mm，1.7 μm)流动相为乙腈 -0.2%甲酸水，梯度洗脱，流速 0.3 mL/min，柱温 35 ℃，进样量 1 μL，检测波长分别为检测波长为 400 nm(羟基红花黄，色素 A)230 nm(芍药苷)320 nm(阿魏酸)和 260 nm(毛蕊异黄酮葡萄糖苷)。结果羟基红花黄色素 A、芍药苷、阿魏酸和毛蕊异黄酮葡萄、糖苷的线性范围、分别为 1.34~26.80 mg/L(r=0.999 8)2.46~49.20 mg/L(r=0.999 9)0.582~11.64 mg/L(r=0.999 9)，0.62~12.40 mg/L(r=0.999 9)；平均加样回收率分别为101.11%(RSD=1，.64%)、 99.62%(RSD=0.79%)，、100.66%(RSD=2.13%)、101.93%(RSD=1.08%)。结论该方法简便、快速、准确性好，可为全面控制芪参还五胶囊的质量提供参考。 |
| 英文摘要: |
| Objective To establish an ultra performance liquid chromatography (UPLC) combined with switching wavelength method for simultaneous determination of four components in Qishen Huanwu capsules including hydroxysafflor yellow A, paeoniflorin, ferulic acid and calycosin-7-glucoside.Methods From May 2021 to January 2022, UPLC was performed on the column of WATERS ACQUITY UPLC BEH C18 (2.1 mm ×50 mm,1.7 μm,), the mobile phase was acetonitrile-0.2% formic acid with gradient elution at the flowrate of 0.3 mL/min. The column temperature was 35 ℃, the injection volume was 1 μL, and the detection wavelengths were 400 nm (hydroxysafflor yellow A), 230 nm (paeoniflorin), 320 nm (ferulic acid) and 260 nm (calycosin-7-glucoside).Results The linear ranges of hydroxysafflor yellow A, ferulic acid, paeoniflorin and calycosin-7-glucoside were 1.34~26.80 mg/L (r=0.999 8), 2.46~49.20 mg/L (r=0.999 9), 0.582~11.64 mg/L (r=0.999 9), 0.62~12.40 mg/L (r=0.999 9), respectively. The average recoveries of the four componentswere 101.1% (RSD=1.6%), 99.6% (RSD=0.79%), 100.7% (RSD=2.1%), 101.9% (RSD=1.1%), respectively.Conclusion The method is simple, accurate and reliable, which can be used for quality control of Qishen Huanwu capsules. |
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