| 张茹,卜倩,贾宏林,等.微 RNA和去乙酰化在支气管哮喘中对 Th细胞因子的影响[J].安徽医药,2023,27(10):1949-1955. |
| 微 RNA和去乙酰化在支气管哮喘中对 Th细胞因子的影响 |
| Effects of miRNA and deacetylation on Th cytokine in bronchial asthma |
| |
| DOI：10.3969/j.issn.1009-6469.2023.10.010 |
| 中文关键词: 哮喘 微 RNA-126 微 RNA-326 曲古抑菌素 A Th细胞因子 |
| 英文关键词: Asthma microRNA-126 microRNA-326 Trichostation A Thcytokine |
| 基金项目:国家自然科学基金项目( 81760182) |
|
| 摘要点击次数: 530 |
| 全文下载次数: 362 |
| 中文摘要: |
| 目的探究支气管哮喘( BA)病人外周血 CD4+T淋巴细胞中微 RNA-126(miR-126)、微 RNA-326(miR-326)和微 RNA155(miR-155)以及 Th细胞相关因子的表达情况；探究去乙酰化酶抑制剂曲古抑菌素 A(TSA)对支气管哮喘大鼠 miR-126、miR326和 miR-155以及 Th细胞相关因子的调控作用。方法收集 2015年 10月至 2017年 12月就诊于新疆医科大学第一附属医院、新疆维吾尔自治区人民医院及新疆医科大学附属中医医院的哮喘急性发作期病人( 48例)采用实时荧光定量 PCR技术检测 BA病人 miR-126、miR-326和 miRNA-155以及 Th细胞相关因子 mRNA的表达情况。建立 BA大，鼠模型，分别给予大鼠低、高浓度的 TSA干预， PCR技术检测各组 miR-126、miR-326和 miR-155以及 Th细胞相关因子的表达。免疫组化法检测干扰素 -γ(IFN-γ)、 GATA结合蛋白 3(GATA3)、维甲酸相关孤儿受体( ROR-γ)、叉头翼状螺旋转录因子 3(Foxp3)蛋白表达。结果 BA病人组 miR-126、miR-326和 miR-155 mRNA的表达高于健康人组( 1.63±0.30比 0.54±0.10，2.25±0.67比 1.62±0.39，2.17±0.68比1.75±0.46，均 P<0.05)IFN-γ和 Foxp3 mRNA表达低于健康人组( 0.86±0.19比 1.36±0.27，1.01±0.22比 1.83±0.30，P<0.05)RORγmRNA表达高于正常组(2.79±0.40比 1.62±0.38，P<0.05)； BA大鼠模型中，模型组中 miR-126 mRNA的表达高于正常组(2.17±0.39比 0.92±0.06，P<0.01)TSA低剂量组中 miR-126 mRNA的表达低于模型组(2.17±0.39比 1.24±0.20，P<0.05)； TSA高剂量组中 GATA3 mRNA的表达低于，正常组和模型组( 3.69±0.88比 1.64±0.35、1.08±0.36，P<0.01)；在 TSA高剂量组中 ROR-γ mRNA的表达高于正常组和模型组( 5.50±1.02比 0.89±0.37、10.94±2.85，P<0.05)。结论去乙酰化酶抑制剂可下调 miR-126、miR-326和 miR-155的表达；适量的去乙酰化酶抑制剂可上调细胞因子 IFN-γ和 Foxp3的表达以及下调 GATA3和 ROR-γ的表达。 |
| 英文摘要: |
| Objective To investigate the expression of microRNA-126 (miR-126), microRNA-326 (miR-326), microRNA-155 (miR155) and Th cell-related factors in peripheral blood CD4+ T lymphocytes from bronchial asthma (BA) patients, and to investigate the regulatory effects of the deacetylase inhibitor trichostation A (TSA) on miR-126, miR-326, miR-155 and Th cell-associated factors in bronchial asthma rats.Methods Collect 48 patients with acute asthma attacks who visited the First Affiliated Hospital of Xinjiang MedicalUniversity, the People′s Hospital of Xinjiang Uygur Autonomous Region, and the Affiliated Traditional Chinese Medicine Hospital ofXinjiang Medical University from October 2015 to December 2017. Real-time fluorescence quantitative PCR was used to detect the expression of miR-126, miR-326, miRNA-155 and Th cell-associated factor mRNA in BA patients. A rat model of BA was establishedand rats were given low and high concentrations of TSA intervention and the expression of miR-126, miR-326, miR-155 and Th cell-related genes in each group was detected by PCR technique. The proteins expression of interferon-γ (IFN-γ), GATA binding protein 3 (GATA3), retinoic acid receptor-related orphan receptor-γ (ROR-γ) and winged-helix transcription factor 3 (Foxp3) were detected by immunohistochemistry. Results The miR-126 and miR-326 and miR-155 mRNA expressions were higher in the BA patient group than those in the healthy people group (1.63±0.30 vs.0.54±0.10, 2.25±0.67 vs. 1.62±0.39, 2.17±0.68 vs. 1.75±0.46, P<0.05), and IFN-γ and Foxp3 mRNA expressions were lower than those in the healthy people group (0.86±0.19 vs. 1.36±0.27, 1.01±0.22 vs. 1.83±0.30, P< 0.05), and ROR-γ mRNA expression was higher than that in the normal group (2.79±0.40 vs. 1.62±0.38, P<0.05). In the BA rat model,miR-126 mRNA expression in the model group was higher than that in the normal group (2.17±0.39 vs. 0.92±0.06, P<0.01), miR-126 mRNA expression in the TSA low-dose group was lower than that in the model group (2.17±0.39 vs. 1.24±0.20, P<0.05). GATA3 mRNA expression in the TSA high-dose group was lower than that in the normal group and the model group (3.69±0.88 vs. 1.64±0.35, 1.08±0.36, P<0.01). ROR-γ mRNA expression was higher in the TSA high-dose group than that in the normal group and the model group (5.50±1.02 vs. 0.89±0.37, 10.94±2.85, P<0.05).Conclusions Deacetylase inhibitors can downregulate the expression of miR126, miR-326 and miR-155. Moderate amount of deacetylase inhibitors can upregulate the expression of cytokine IFN-γ and Foxp3 and downregulate the expression of GATA3 andROR-γ. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
