| 张红素,王林,冯梅.孕妇胎盘多巴胺 D2受体、锚蛋白重复和激酶域 1表达与妊娠期糖尿病巨大儿的关系[J].安徽医药,2023,27(10):2072-2076. |
| 孕妇胎盘多巴胺 D2受体、锚蛋白重复和激酶域 1表达与妊娠期糖尿病巨大儿的关系 |
| Relationship between the expression of DRD2 and ANKK1 in the placenta of pregnant women and macrosomia due to gestational diabetes mellitus |
| |
| DOI：10.3969/j.issn.1009-6469.2023.10.036 |
| 中文关键词: 糖尿病，妊娠 出生体质量 多巴胺 D2受体 锚蛋白重复和激酶域 1 巨大儿 |
| 英文关键词: Diabetes,gestational Birth weight Dopamine receptor D2 Ankyrin repeat and kinase domain containing 1 Giant infants |
| 基金项目: |
|
| 摘要点击次数: 428 |
| 全文下载次数: 345 |
| 中文摘要: |
| 目的探究妊娠期糖尿病( GDM)孕妇胎盘组织中多巴胺 D2受体( DRD2)、锚蛋白重复和激酶域 1(ANKK1)的表达情况，及二者与 GDM巨大儿的关系。方法选取 2018年 3月至 2020年 12月在西北妇女儿童医院足月分娩的 185例孕妇为研究对象，按照孕妇是否存在 GDM、新生儿是否为巨大儿分为对照组(无 GDM、新生儿体质量正常)、正常巨大儿组、 GDM正常体质量组及 GDM巨大儿组。分别采用免疫组化法及 qRT-PCR法检测胎盘组织中 DRD2、ANKK1蛋白及 mRNA表达，并采用 Pearson法分析 DRD2、ANKK1表达与 GDM巨大儿的相关性，采用 logistic回归分析影响 GDM孕妇生产巨大儿的因素。结果对照组、正常巨大儿组、 GDM正常体质量组、 GDM巨大儿组胎盘组织中 DRD2蛋白阳性率分别为 87.76%(43/49)、 74.47%(35/47)、62.22%(28/45)、 52.27%(23/44)， DRD2 mRNA分别为( 1.03±0.06)、(0.86±0.14)、(0.71±0.15)、(0.58±0.11)，四组胎盘组织中 DRD2蛋白阳性率及 mRNA水平由高到低分别是对照组、正常巨大儿组、 GDM正常体质量组和 GDM巨大儿组，四组比较差异有统计学意义( P<0.05)且 DRD2 mRNA水平组间两两比较差异有统计学意义(P<0.05)；对照组、正常巨大儿组、 GDM正常体质量组、 GDM巨大儿组胎组织中 ANKK1蛋白阳性率分别为 44.90%(22/49)、 59.57%(28/47)、 71.11%(32/45)、 81.82%(36/44)， ANKK1 mRNA分别为 1.01±0.05、1.24±0.27、1.53±0.39、1.82±0.46，四组胎盘组织中 ANKK1蛋白阳性率及 mRNA水平由高到低分别是 GDM巨大儿组、 GDM正常体质量组、正常巨大儿组和对照组，四组比较差异有统计学意义(P<0.05)且 ANKK1 mRNA水平组间两两比较差异有统计学意义(P<0.05)。Pearson相关性分析显示，胎盘组织中 DRD2表达与新生儿体呈负相关(r=?0.32， P=0.021)ANKK1表达与新生儿体质量呈正相关( r=0.34，P=0.016)。logistic回归分析结果显示， DRD2 mRNA、ANKK1mRNA均为 GDM生产巨大儿的影响因素。结论 GDM孕妇胎盘组织中 DRD2呈低表达， ANKK1呈高表达，二者水平均与新生儿体盘，质量，孕妇，质量相关。 |
| 英文摘要: |
| Objective To explore the expression of dopamine receptor D2 (DRD2), ankyrin repeat and kinase domain containing 1(ANKK1) in placental tissues of pregnant women with gestational diabetes mellitus (GDM), and their relationship with GDM giant infants.Methods A total of 185 pregnant women who gave birth at full-term at Northwest Women′s and Children′s Hospital from March2018 to December 2020 were selected as the research objects. According to whether the pregnant women had GDM and whether thenewborns were giant infants, they were assigned into control group (no GDM, normal newborn weight), normal giant infant group, andGDM normal weight group and GDM giant infant group. Immunohistochemistry and qRT-PCR were used to detect the levels of DRD2,ANKK1 protein and mRNA in placental tissues, and Pearson method was used to analyze the correlation between DRD2, ANKK1 expression and GDM giant infant. Logistic regression was used to analyze the factors affecting the birth of giant infant in pregnant womenwith GDM.Results The positive rates of DRD2 protein in placenta tissues of the control group, normal giant infant group, GDM normal weight group, and GDM giant infant group were 87.76% (43/49), 74.47% (35/47), 62.22% (28/45), and 52.27% (23/44), respectively, and DRD2 mRNA were (1.03±0.06), (0.86±0.14), (0.71±0.15), and (0.58±0.11), respectively; the positive rates of DRD2 protein andlevel of mRNA in the placenta tissues from high to low were in the control group, the normal giant infant group, the GDM normal weightgroup and the GDM giant infant group, the difference between the four groups was statistically significant (P<0.05), and the difference between any two groups of DRD2 mRNA expression was statistically significant (P<0.05); the positive rates of ANKK1 protein in placenta tissues of the control group, normal giant infant group, GDM normal weight group, and GDM giant infant group were 44.90% (22/49), 59.57% (28/47), 71.11% (32/45), and 81.82% (36/44), respectively, and ANKK1 mRNA were 1.01±0.05, 1.24±0.27, 1.53±0.39,and 1.82±0.46, respectively; the positive rates of ANKK1 protein and mRNA levels in the placental tissues from high to low were in the GDM giant infant group, GDM normal weight group, normal giant infant group, and control group, the difference between the four groups was statistically significant (P<0.05), and the difference between any two groups of ANKK1 mRNA expression was statistically significant (P<0.05). Pearson correlation analysis showed that DRD2 expression in placental tissue was negatively correlated with newborn weight (r=?0.32, P=0.021), and ANKK1 expression was positively correlated with newborn weight (r=0.34, P=0.016). The logistic regression analysis showed that DRD2 mRNA and ANKK1 mRNA were the influencing factors of GDM pregnant women giving birth to giant babies.Conclusion DRD2 is lowly expressed in placenta tissue of GDM pregnant women, while ANKK1 is highly expressed.Both levels are correlated with newborn weight. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
