文章摘要
滕玉环,徐晨辉,陈季南,等.血清长链非编码 RNA小核仁 RNA宿主基因 1、微 RNA-329-3p表达水平与急性脑梗死发生风险的关系[J].安徽医药,2023,27(11):2171-2175.
血清长链非编码 RNA小核仁 RNA宿主基因 1、微 RNA-329-3p表达水平与急性脑梗死发生风险的关系
Relationship between expression levels of serum long non-coding RNA small nucleolar RNA host gene 1 and miR-329-3p and the risk of acute cerebral infarction
  
DOI:10.3969/j.issn.1009-6469.2023.11.011
中文关键词: 脑梗死  长链非编码 RNA  微 RNA-329-3p  风险
英文关键词: Brain infarction  Long non-coding RNA  MiR-329-3p  Risk
基金项目:南京医科大学科技发展基金( NMUB2019240)
作者单位
滕玉环 南京市江宁医院神经内科江苏南京 211100 
徐晨辉 南京市江宁医院神经内科江苏南京 211100 
陈季南 南京市江宁医院神经内科江苏南京 211100 
李军荣 南京市江宁医院神经内科江苏南京 211100 
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中文摘要:
      目的探究急性脑梗死( ACI)病人血清长链非编码 RNA小核仁 RNA宿主基因 1(lncRNA SNHG1)、微 RNA-329-3p(miR-329-3p)表达水平变化及其与疾病发生风险的关系。方法选取 2020年 9月至 2021年 9月在南京市江宁医院收治的 162例 ACI病人作为观察组,根据病人美国国立卫生研究院卒中量表( NIHSS)分为轻度 ACI组 60例( NIHSS≤7分)、中度 ACI组 56例(8分≤NIHSS≤13分)和重度 ACI组 46例( NIHSS≥14分),另选取同时期在南京市江宁医院体检的 165例健康志愿者作为对照组;采用实时荧光定量 PCR(qRT-PCR)检测血清 lncRNA SNHG1、miR-329-3p水平;采用 Spearman法分析 lncRNA SNHG1、miR329-3p与 NIHSS评分的关系;采用 Pearson法分析 lncRNA SNHG1与 miR-329-3p水平的关系;采用 ROC曲线分析 lncRNA SNHG1、miR-329-3p对 ACI的诊断价值;多因素 logistic回归分析影响 ACI发生的危险因素。结果与对照组( 1.03±0.04、1.01±0.03)相比,观察组病人血清 lncRNA SNHG1表达水平( 0.34±0.08)显著降低( P<0.05)miR-329-3p水平( 2.15±0.70)明显升高( P <0.05); lncRNA SNHG1表达水平随疾病严重程度增加而显著降低( 0.43±0.09、0.35±0.21±0.07)(P<0.05)miR-329-3p水平.08、0,随疾病严重程度增加而明显升高( 1.51±0.50、2.20±0.69、2.92±0.97)(P<0.05); ACI病人血清 lncRNA SNHG1水平,与 NIHSS评分呈负相关( P<0.05)miR-329-3p水平与 NIHSS评分呈正相关( P<0.05),lncRNA SNHG1与 miR-329-3p表达呈负相关( P<0.05); ROC曲线分析表明,,与 lncRNA SNHG1单独诊断相比,二者联合诊断 ACI发生的 ROC曲线下面积( AUC)差异无统计学意义(Z=1.33,P=0.920),与 miR-329-3p单独诊断相比,二者联合诊断 ACI发生的 AUC更高( Z=2.93,P=0.003);多因素 logistic回归分析显示, lncRNA SNHG1低水平、 miR-329-3p高水平是影响 ACI发生的危险因素( P<0.05)。结论 ACI病人血清 lncRNA SNHG1表达水平降低, miR-329-3p表达水平升高,且与病人病情严重程度有关。
英文摘要:
      Objective To investigate the changes in the expression levels of long non-coding RNA small nucleolar RNA host gene 1 (lncRNA SNHG1) and miR-329-3p in serum of patients with acute cerebral infarction (ACI) and their relationship with the risk of dis‐ease.Methods A total of 162 patients with ACI admitted to Nanjing Jiangning Hospital from September 2020 to September 2021 weregathered as the study group. According to the national institute of health stroke scale (NIHSS), the patients were assigned into a mildACI group of 60 cases (NIHSS≤7 points), a moderate ACI group of 56 cases (8 points≤NIHSS≤13 points), and a severe ACI group of 46cases (NIHSS≥14 points). In addition, 165 healthy volunteers who received physical examination in our hospital were gathered as thecontrol group. Real-time fluorescent quantitative PCR (qRT-PCR) was performed to measure the levels of serum lncRNA SNHG1 and miR-329-3p; Spearman method was used to analyze the relationship between lncRNA SNHG1, miR-329-3p and NIHSS score; Pearson method was used to analyze the relationship between lncRNA SNHG1 and miR-329-3p level; ROC curve was performed to analyze the diagnostic value of lncRNA SNHG1 and miR-329-3p for ACI; multivariate logistic regression was performed to analyze the risk factors affecting the occurrence of ACI.Results Compared with the control group (1.03±0.04, 1.01±0.03), the expression level of serum lncRNA SNHG1 (0.34±0.08) in the study group was obviously reduced (P<0.05), and the level of miR-329-3p (2.15±0.70) was obviously increased (P<0.05); the expression level of lncRNA SNHG1 decreased obviously with the severity of the disease increased (0.43±0.09,0.35±0.08, 0.21±0.07) (P<0.05), and the level of miR-329-3p increased obviously with the severity of the disease increased (1.51±0.50, 2.20±0.69, 2.92±0.97) (P<0.05); the level of serum lncRNA SNHG1 in ACI patients was negatively correlated with NIHSS score (P< 0.05), the level of miR-329-3p was positively correlated with NIHSS score (P<0.05), and the expression of lncRNA SNHG1 and miR329-3p was negatively correlated (P<0.05); ROC curve analysis showed that compared with the diagnosis of lncRNA SNHG1 alone, thearea under the ROC curve (AUC) of the combined diagnosis of ACI was not obviously different (Z=1.33, P=0.920), while compared with the diagnosis of miR-329-3p alone, the AUC of the two combined diagnosis of ACI was higher (Z=2.93, P=0.003); multivariate logistic regression analysis showed that low level of lncRNA SNHG1 and high level of miR-329-3p were risk factors affecting the occurrence of ACI (P<0.05).Conclusion The expression level of serum lncRNA SNHG1 in ACI patients is decreased, and the expression level of miR-329-3p is increased, which is related to the severity of the disease of the patients.
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