| 王璐,张媛,罗静,等.氧化苦参碱通过影响辅助性 T细胞 17/调节性 T细胞的比例对急性淋巴细胞白血病小鼠免疫功能的影响[J].安徽医药,2023,27(12):2355-2360. |
| 氧化苦参碱通过影响辅助性 T细胞 17/调节性 T细胞的比例对急性淋巴细胞白血病小鼠免疫功能的影响 |
| Effect of oxymatrine on immune function of acute lymphoblastic leukemia mice by influencing Th17/Treg ratio |
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| DOI：10.3969/j.issn.1009-6469.2023.12.006 |
| 中文关键词: 苦参 免疫功能 Th17/Treg细胞: 急性淋巴细胞白血病 小鼠，近交 NOD |
| 英文关键词: Lightyellow sophora root Immune function Th17/Treg cells Acute lymphoblastic leukemia Mice, Inbred NOD |
| 基金项目:2019年度河南省医学科技攻关计划项目(LHGJ20190049) |
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| 中文摘要: |
| 目的探讨氧化苦参碱(OMT)通过影响辅助性 T细胞 17(Th17)/调节性 T细胞(Treg)的比例对急性淋巴细胞白血病小鼠免疫功能的影响。方法 2021年 6—12月将 50只小鼠按随机数字表法分为正常对照组、非干预组、OMT低、中、高剂量组，常对照组灌胃等量生理盐水，其余四组小鼠于右前侧皮下注射 L1210细胞悬浮液建立急性淋巴细胞白血病(ALL)小鼠模型。正造模成功后进行干预治疗，OMT低、中、高剂量组分别灌胃 50、100、200 mg/kg剂量的 OMT，正常对照组和非干预组注射灌胃生理盐水。采用人工计数法进行白细胞计数及分类；计算脾脏和胸腺指数；ELISA法检测小鼠血清中白细胞介素(IL)-17、IL-22、转化生长因子-β(TGF-β)和 IL-10水平；流式细胞术检测 CD4＋、CD8＋百分比、CD4＋/CD8＋及 Th17、Treg比例。结果 OMT可增加小鼠体质量、中性粒细胞数量、CD4＋/CD8＋脾脏和胸腺指数，降低皮下瘤质量、L1210细胞、淋巴细胞、白细胞数量及 Th17/Treg。正常对照组、非干预组、OMT低、中、高剂量、组 IL-17水平分别为(70.81±6.24)ng/L、(257.34±22.93)ng/L、(189.36±18.54)ng/L、(124.47±12.73)ng/L、(81.13±7.39)ng/L；正常对照组、非干预组、OMT低、中、高剂量组 IL-22水平分别为(66.29±6.85)ng/L、(245.08±22.93)ng/L、(153.43±17.29)ng/L、(101.30±11.33)ng/L、(77.87±6.74)ng/L；正常对照组、非干预组、OMT低、中、高剂量组 TGF-β水平分别为(281.94±25.91)ng/L、(71.01±7.25)ng/L、(90.39±10.83)ng/L、(142.00±16.00)ng/L、(221.66±19.59)ng/L；正常对照组、非干预组、OMT低、中、高剂量组 IL-10水平分别为(157.68±13.34)ng/L、(42.74±4.80)ng/L、(58.23±6.32)ng/L、(80.14±8.73) ng/L、(96.27±10.87)ng/L。结论 OMT能够提高 ALL小鼠免疫功能，其机制可能与改善 Th17/Treg细胞平衡有关。 |
| 英文摘要: |
| Objective To explore the effect of oxymatrine (OMT) on immune function of acute lymphoblastic leukemia mice by af. fecting the ratio of T-helper cell 17 (Th17)/regulatory-T cell (Treg).Methods From June 2021 to December, 2021, 50 mice were ran. domly assigned into normal control group, non-intervention group, low-, medium-, and high-dose OMT groups according to random num.ber table method. Normal control group was given equal amount of intragastric normal saline, and the remaining four groups were subcu.taneously injected with L1210 cell suspension in the right anterior side to establish acute lymphoblastic leukemia (ALL) mice model.After successful modeling, intervention was performed. The low-, medium-, and high-dose OMT groups were given 50, 100 and 200 mg/ kg of OMT, respectively. The mice of normal control group and non-intervention group were given normal saline. White blood cell countand classification were carried out by manual counting method. Spleen and thymus indexes were calculated. Serum levels of interleukin(IL)-17, IL-22, transforming growth factor-β (TGF-β) and interleukin-10 (IL-10) were detected by the enzyme linked immunosorbent as. |
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