| 董克臣,张萌,梁毅,等.康复新液调控巨噬细胞极化防治大鼠急性放射性口腔黏膜炎[J].安徽医药,2023,27(12):2366-2370. |
| 康复新液调控巨噬细胞极化防治大鼠急性放射性口腔黏膜炎 |
| Kangfuxin liquid regulates macrophage polarization to prevent acute radiation-induced oral mucositis in rats |
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| DOI：10.3969/j.issn.1009-6469.2023.12.008 |
| 中文关键词: 黏膜炎 康复新液 大鼠， Sprague-Dawley 转化生长因子 -β 放射性口腔黏膜炎 巨噬细胞 极化 |
| 英文关键词: Mucositis Kangfuxin liquid Rats, Sprague-Dawley Transforming growth factor beta Radiation-induced oral mucositis Macrophages Polarization |
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| 中文摘要: |
| 目的探讨康复新液对大鼠急性放射性口腔黏膜炎( ROM)的防治效果及对巨噬细胞极化的影响。方法该研究于 2021年 7月至 2022年 4月进行，将 54只 SD大鼠按随机数字表法分成对照组、照射组和给药组，各 18只。除对照组外，其余各组均行单次大剂量照射模拟急性 ROM。给药组予康复新液，其余两组给予生理盐水。观察照射后大鼠一般情况及颊黏膜病理变化。酶联免疫吸附测定( ELISA)检测颊黏膜组织中白细胞介素( IL)-6、肿瘤坏死因子 -α(TNF-α)、转化生长因子 -β(TGF-β)和 IL-10水平。免疫荧光法检测颊黏膜组织 CD86和 CD206阳性巨噬细胞。逆转录聚合酶链反应(RT-PCR)和蛋白质印迹法检测颊黏膜中诱导型一氧化氮合酶( iNOS)、精氨酸酶 1(Arg1)、共刺激分子 CD86和 CD206 mRNA表达和蛋白水平。结果与照射组比较，给药组大鼠一般情况得到改善，颊黏膜炎症减轻。对照组、照射组和给药组颊黏膜组织 IL-6浓度分别为( 0.46±0.04)μg/L、(1.12±0.16)μg/L、(0.74±0.08)μg/L，TNF-α浓度分别为( 0.12±0.02)μg/L、(0.68±0.10)μg/L、(0.34±0.06)μg/L，TGF-β浓度分别为( 0.32±0.06)μg/L、(0.75±0.12)μg/L、(1.26±0.20)μg/L，IL-10浓度分别为( 0.58±0.08)μg/L、(0.96±0.14)μg/L、(1.44±0.21)μg/L；三组 CD86阳性巨噬细胞数目分别为 0、152.54±6.95、99.51±3.61，CD206阳性细胞数目分别为 0、88.26±3.10、129.99± 2.61；三组 |
| 英文摘要: |
| Objective To explore the protective effect of kangfuxin liquid on acute radiation-induced oral mucositis (ROM) in rats and its impact on macrophage polarization.Methods This study was conducted from July 2021 to April 2022. Totally 54 SD rats wererandomized into control group, radiation group and administration group, with 18 rats in each group. Except for the control group, theother two groups were irradiated at a single high-dose to make acute ROM model. The administration group was given kangfuxin liquid,while radiation group and control group were given saline solution. The general conditions and histopathological changes of buccal mu.cosa were observed after irradiation. The levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), tumor growth factor-β (TGF-β), and interleukin-10 (IL-10) of the mucosal tissues were measured by enzyme-linked immunosorbent assay (ELISA). CD86 andCD206 positive macrophages in buccal mucosa were detected by immunofluorescence. The mRNA expressions and protein levels of in.ducible nitric oxide synthase (iNOS), arginase 1 (Arg1), costimulatory molecules CD86 and CD206 in buccal mucosa were detected byreverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, respectively.Results Compared with radiation group,the general condition of rats in administration group was improved, and the inflammation of mucosal tissue was alleviated. The concen.trations (μg/L) of IL-6, TNF-α, TGF-β, and IL-10 in the control, radiation and administration groups were [(0.46±0.04) μg/L, (1.12±0.16) μg/L, (0.74±0.08) μg/L]; [(0.12±0.02) μg/L, (0.68±0.10) μg/L, (0.34±0.06) μg/L]; [(0.32±0.06) μg/L, (0.75±0.12) μg/L, (1.26±0.20) μg/L]; [(0.58±0.08) μg/L, (0.96±0.14) μg/L, (1.44±0.21) μg/L], respectively. The numbers of CD86 positive macrophages in eachgroup were 0, 152.54±6.95, 99.51±3.61) respectively; and the numbers of CD206 positive macrophages in each group were 0, 88.26±3.10, 129.99±2.61, respectively. The relative mRNA expression of iNOS, CD86, Arg1, and CD206 were (0.004 43±0.000 25, 0.006 82±0.000 41, 0.005 94±0.000 38); (0.003 42±0.000 20, 0.005 84±0.000 43, 0.004 22±0.000 35); (0.004 24±0.000 16, 0.005 20±0.000 55,0.006 61±0.000 53); (0.004 23±0.000 38, 0.006 82±0.000 56, 0.008 24±0.000 86), respectively. Kangfuxin liquid could significantlyalleviate the buccal mucosa inflammation with acute ROM, inhibite M1 macrophage polarization, promote M2 macrophage polarization,reduce the expressions of IL-6 and TNF-α, and promote the release of TGF-β and IL-10 (P<0.05).Conclusions Kangfuxin liquid canimprove the general condition of acute ROM rats and reduce the inflammation of oral mucosa. It may inhibit M1 polarization of macro.phages in buccal mucosa and promote M2 polarization, thereby inhibiting the expressions of proinflammatory factors and upregulatingthe expressions of anti-inflammatory factors, thus reducing the inflammatory reaction of oral mucosa. |
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