| 司新成,苗鹏飞,辛艳峰,等.血清肺腺癌转录相关转录本 1、微 RNA-1水平与心肌梗死继发室性心律失常的关系研究[J].安徽医药,2023,27(12):2469-2474. |
| 血清肺腺癌转录相关转录本 1、微 RNA-1水平与心肌梗死继发室性心律失常的关系研究 |
| Relationship between serum MALAT1 and miR-1 levels and ventricular arrhythmia secondary to myocardial infarction |
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| DOI：10.3969/j.issn.1009-6469.2023.12.030 |
| 中文关键词: 心肌梗死 心律失常，心性 肺腺癌转录相关转录本 1 微 RNA-1 肌酸激酶 |
| 英文关键词: Myocardial infarction Arrhythmias, cardiac Metastasis-associated lung adenocarcinoma transcript 1 MicroRNA-1 Creatine Kinase |
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| 中文摘要: |
| 目的探讨血清肺腺癌转录相关转录本 1(MALAT1)、微 RNA-1(miR-1)水平与急性心肌梗死(AMI)继发室性心律失常(VA)的关系。方法选取 2019年 10月至 2021年 6月临汾市中心医院收治的 AMI病人 270例，均行经皮冠状动脉介入治疗(PCI)治疗，术后进行 24 h动态心电图监测，根据 Lown分级将病人分为心律正常组(0级，166例)和心律失常组(1~5级，104例)。收集所有病人一般资料，实时荧光定量逆转录聚合酶链反应(qRT-PCR)法检测血清 MALAT1、miR-1水平，Pearson法分析 AMI继发 VA病人血清 MALAT1水平与 miR-1水平的相关性，采用受试者操作特征(ROC)曲线分析血清 MALAT1、miR-1水平对 AMI病人继发 VA的诊断价值，并分析 AMI病人继发 VA的影响因素。结果心律失常组心肌标志物肌酸激酶同工酶(CK-MB)血清 N末端脑钠肽前体(NT-proBNP)、心肌肌钙蛋白(cTnI)、左心室舒张末期内径(LVEDD)水平、Gensini积分、右冠脉病变比例、及血清 MALAT1(1.28±0.34)、miR-1(1.24±0.33)水平明显高于心律正常组(1.01±0.12、0.99±0.10)(P<0.05)左心室射血分数(LVEF)水平明显低于心律正常组(P<0.05)；心律失常 5级、4级病人血清 MALAT1(1.76±0.47、1.45±0.40)、m，iR-1 (1.69±0.43、1.40±0.39)水平明显高于 3级( 1.12±0.30、1.09±0.31)、 2级( 1.08±0.29、1.05±0.26)、 1级( 1.04±0.27、1.02±0.25)病人(P<0.05)，心律失常 5级病人血清 MALAT1(1.76±0.47)、 miR-1(1.69±0.43)水平明显高于 4级( 1.45±0.40、1.40±0.39)病人( P<0.05)； AMI继发 VA病人血清 MALAT1水平与 miR-1水平呈正相关( P<0.05)；血清 MALAT1、miR-1水平诊断 AMI继发 VA的曲线下面积分别为 0.77、0.81，灵敏度分别为 69.2%、73.1%，特异度分别为 81.9%、88.0%，最佳截断值分别为 1.13、1.15；二者联合诊断 AMI继发 VA的曲线下面积、灵敏度、特异度分别为 0.90、84.6%、86.7%，二者联合诊断的曲线下面积高于二者单独诊断(P<0.05)； logistic回归分析结果显示，高 CK-MB、高 NT-proBNP、高 cTnI、高 LVEDD、高 Gensini积分、右冠脉病变、低 LVEF及血清 MALAT1高表达、 miR-1高表达为 AMI病人继发 VA的独立危险因素(P<0.05)。结论 AMI继发 VA病人血清 MALAT1、miR-1水平升高，二者均为 AMI病人继发 VA的独立危险因素，可用于诊断 AMI病人是否继发 VA，联合检测诊断价值更高。 |
| 英文摘要: |
| Objective To investigate the relationship between serum levels of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), microRNA-1 (miR-1) and ventricular arrhythmia (VA) secondary to acute myocardial infarction (AMI).Methods A total of 270 AMI patients admitted to Linfen Central Hospital from October 2019 to June 2021 were treated with percutaneous coronary inter.vention (PCI) followed by 24-hour dynamic ECG monitoring. According to Lown grading system, the patients were assigned into normalgroup (grade 0, 166 cases) and arrhythmia group (grade 1~5, 104 cases). The general information of all patients was collected. Real-time fluorescent quantitative reverse transcription polymerase chain reaction (qRT-PCR) method was used to detect the serum MALAT1 and miR-1 levels, Pearson method was used to analyze the correlation between serum MALAT1 level and miR-1 level in patients with VA secondary to AMI, ROC curve was used to analyze the diagnostic value of serum MALAT1 and miR-1 levels in the diagnosis of second. ary VA in AMI patients, and the influencing factors of secondary VA in AMI patients.Results The levels of myocardial marker cre. atine kinase isoenzyme (CK-MB), serum N-terminal brain natriuretic peptide precursor (NT-proBNP), cardiac troponin (cTnI), left ven.tricular end diastolic dimension (LVEDD), Gensini score, the proportion of right coronary artery lesions and the levels of serumMALAT1and miR-1 [(1.28±0.34), (1.24±0.33)] in arrhythmia group were significantly higher than those in normal group (1.01±0.12, 0.99±0.10) (P<0.05), and the level of left ventricular ejection fraction (LVEF) was significantly lower in arrhythmia group than normal group (P<0.05). The levels of serum MALAT1 (1.76±0.47, 1.45±0.40) and miR-1 (1.69±0.43, 1.40±0.39) in patients with arrhythmiagrade 5 and 4 were significantly higher than those in patients with arrhythmia grade 3 (1.12±0.30, 1.09±0.31), 2 (1.08±0.29,1.05±0.26)and 1 (1.04±0.27,1.02±0.25) (P<0.05). The levels of serum MALAT1 and miR-1 in patients with arrhythmia grade 5 were significantly higher than those in patients with arrhythmia grade 4 [(1.76±0.47) vs. (1.45±0.40), (1.69±0.43) vs. (1.40±0.39); P<0.05]. The serum MALAT1 level of patients with VA secondary to AMI was positively correlated with their miR-1 level (P<0.05). The areas under the curve of serum MALAT1 and miR-1 levels for the diagnosis of VA secondary to AMI were 0.77 and 0.81, the sensitivities were 69.2%and 73.1%, the specificities were 81.9% and 88.0%, and the best cutoff values were 1.13 and 1.15, respectively. The area under thecurve, sensitivity, and specificity of the combined diagnosis of VA secondary to AMI were 0.90, 84.6%, and 86.7%, respectively; the ar.ea under the curve of combined diagnosis was higher than that of single diagnosis (P<0.05). Logistic regression analysis results showed that high levels of CK-MB, NT-proBNP, cTnI, LVEDD, and Gensini score, right coronary artery disease, low LVEF, and high expres.sions of serum MALAT1 and miR-1 were independent risk factors for secondary VA in patients with AMI (P<0.05).Conclusion The levels of serum MALAT1 and miR-1 in patients with VA secondary to AMI are elevated, both of which are independent risk factors forsecondary VA in AMI patients, and can be used for the diagnosis of secondary VA. The combined detection is of higher diagnostic value. |
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