| Objective To explore the influence of crocin on the malignant biological behavior of colorectal cancer cells by inhibitingthe sonic hedgehog (Shh) signaling pathway.Methods From January 2021 to July 2022,Human colorectal cancer cells SW620 werecultured in vitro and treated with crocin at different concentrations (5,10,20,40,60,80 mg/L) for 24 h.The cytotoxic effects of crocin onSW620 cells were detected by CCK-8 assay. SW620 cells were treated with different doses of crocin (10, 20, 40 mg/L, crocin L/M/Hgroup), 40 mg/L crocin + 1 μmol/L Purmorphamine (crocin H+Shh pathway activator group), 1 μmol/L Purmorphamine (Shh pathwayactivator group), and an untreated control group was set up. The ability of proliferation, apoptosis, migration and invasion of SW620cells and the expression of Shh signaling pathway related proteins Shh, smooth protein (Smo) and glioma associated oncogene-1 (Gli-1) were detected.Results Crocin decreased SW620 cell viability in a dose-dependent manner. Compared with the Control group, the via.bility of SW620 cells in the Shh pathway activator group was increased, the number of clone formation, the healing rate of scratch area,the number of invasion, the expression of Shh (1.47±0.09 vs. 1.27±0.08), Smo (1.38±0.12 vs. 1.15±0.09) and Gli-1 (1.25±0.09 vs. 0.98± 0.07) proteins were increased, and the apoptosis rate was decreased (P<0.05). the viability of SW620 cells in the crocin L group, thecrocin M group and the crocin H group decreased, the number of colonies, the healing rate of the scratch area, and the number of inva.sion decreased, the apoptosis rate increased, and the protein expression of Shh (1.07±0.12, 0.85±0.11, 0.52±0.08 vs. 1.32±0.14), Smo (0.96±0.11, 0.72±0.08, 0.43±0.06 vs. 1.19±0.12) and Gli-1 (0.76±0.09, 0.61±0.09, 0.37±0.06 vs. 0.96±0.11) decreased (P<0.05); com.pared with the crocin H group, the viability of SW620 cells in the crocin H+Shh pathway activator group increased, the number of colo.nies, the healing rate of the scratch area, and the number of invasion increased, the apoptosis rate decreased, and the protein expression of Shh (0.84±0.08 vs. 0.52±0.08), Smo (0.81±0.09 vs. 0.43±0.06) and Gli-1 (0.70±0.08 vs. 0.37±0.06) increased (P<0.05).Conclusion Crocin can inhibit the proliferation, migration and invasion of SW620 cells and promote their apoptosis, which may be related to the in.hibition of Shh signaling pathway. |
