| 符鑫,刘悦,彭鑫,等.非酒精性脂肪性肝病病人血清长链非编码 RNA人类白细胞抗原复合体 18水平与氧化应激指标、肝纤维化程度的相关性[J].安徽医药,2024,28(4):768-772. |
| 非酒精性脂肪性肝病病人血清长链非编码 RNA人类白细胞抗原复合体 18水平与氧化应激指标、肝纤维化程度的相关性 |
| Correlation of serum lncRNA HCG18 level with oxidative stress indicators and liver fibrosis in patients with nonalcoholic fatty liver disease |
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| DOI：10.3969/j.issn.1009-6469.2024.04.028 |
| 中文关键词: 脂肪肝 非酒精性脂肪性肝病 长链非编码 RNA 人类白细胞抗原复合体 18 氧化应激 肝纤维化 |
| 英文关键词: Fatty liver Non-alcoholic fatty liver disease Long non-coding RNA Human leukocyte antigen complex 18 Oxida?tive stress Liver fibrosis |
| 基金项目:湖北省自然科学基金项目( WJ2019A030) |
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| 中文摘要: |
| 目的探究非酒精性脂肪性肝病( NAFLD)病人血清长链非编码 RNA人类白细胞抗原复合体 18(lncRNA HCG18)与氧化应激指标及肝纤维化程度的相关性。方法以儋州市人民医院 2020年 11月至 2022年 3月收治的 92例 NAFLD病人为 NAFLD组，另选取同期健康体检志愿者 90例为对照组。根据 NAFLD肝纤维化( NFS)评分将 NAFLD组病人分为排除晚期纤维化组( 30例)、中间状态组( 46例)、晚期纤维化组( 16例)。采用实时荧光定量 PCR(qRT-PCR)法检测血清 lncRNA HCG18表达水平；酶联免疫吸附测定( ELISA)检测氧化应激指标水平； Pearson相关性分析 NAFLD病人血清 lncRNA HCG18与氧化应激指标的关系； Spearman相关性分析 NAFLD病人血清 lncRNA HCG18与 NFS评分的关系。采用受试者操作特征( ROC)曲线分析血清 lncRNA HCG18对 NAFLD的诊断价值。结果 NAFLD组 lncRNA HCG18(1.37±0.29比 1.01±0.24)、丙二醛［(6.96±2.13) μmol/L比( 4.17±1.04)μmol/L］水平明显高于对照组( P<0.001)，谷胱甘肽过氧化物酶( GSH-Px)［(72.39±11.26)U/mL比( 98.25±13.92)U/mL］超氧化物歧化酶( SOD)［(68.36±10.65)U/mL比( 92.84±12.89)U/mL］水平明显低于对照组( t=9.11、11.19、13.79、13.98，P<0.001。排除晚期纤维化组、中间状态组、晚期纤维化组 lncRNA HCG18(1.20±0.25比 1.39±0.30比 1.65±0.35)、丙二醛［(5.64±1.88)μmol/L比( 7.12±2.16)μmol/L比( 8.99±2.51)μmol/L］水平依次显著升高( F=12.36、13.06，P<0.001)， GSH-Px［(81.56±13.58)U/mL比( 71.74±10.52)U/mL比( 57.06±9.05)U/mL］、SOD［(81.45±12.52)U/mL比( 66.52±10.25)U/mL比( 49.13±8.29)U/mL］水平依次显著降低( F=24.27、48.42，P<0.001)。 Pearson相关性分析显示， NAFLD组血清 lncRNA HCG18水平与丙二醛呈正相关( r=0.59，P<0.001)与 GSH-Px、SOD均呈负相关( r=?0.57、?0.62，P<0.001)； Spearman相关性分析显示 NAFLD组血清 lncRNA HCG18水平与 NFS评分，呈正相关( r=0.69，P<0.001)。血清 lncRNA HCG18诊断 NAFLD的 ROC曲线下面积( AUC)为 0.85。结论 NAFLD病人血清 lncRNA HCG18显著升高，与氧化应激指标及肝纤维化程度存在一定的相关性。 |
| 英文摘要: |
| Objective To explore the correlation of serum long non-coding RNA human leukocyte antigen complex group 18 (ln?cRNA HCG18) with oxidative stress indicators and the degree of liver fibrosis in patients with nonalcoholic fatty liver disease (NAFLD).Methods Ninety-two NAFLD patients admitted to Danzhou People's Hospital from November 2020 to March 2022 were chosen asthe NAFLD group, and 90 healthy volunteers as the control group. According to the NAFLD liver fibrosis score (NFS), the patients inthe NAFLD group were assigned into the exclusion of advanced fibrosis group (n=30), the intermediate state group (n=46), and the ad? vanced fibrosis group (n=16). The expression level of serum lncRNA HCG18 was detected by real-time fluorescence quantitative PCR (qRT-PCR), and the levels of oxidative stress indicators was detected by enzyme-linked immunosorbent assay (ELISA). Pearson correla?tion was used to analyze the relationship between serum lncRNA HCG18 and oxidative stress indicators in NAFLD patients, and Spear?man correlation was used to analyze the relationship between serum lncRNA HCG18 and NFS score in NAFLD patients. Receiver oper?ating characteristic (ROC) curve was used to analyze the diagnostic value of serum lncRNA HCG18 for NAFLD.Results The levels of lncRNA HCG18 [(1.37±0.29) vs. (1.01±0.24)] and malondialdehyde (MDA) [(6.96±2.13) μmol/L vs. (4.17±1.04) μmol/L] in the NAFLDgroup were obviously higher than those in the control group, while the levels of glutathione peroxidase (GSH-Px) [(72.39±11.26) U/mL vs. (98.25±13.92) U/mL] and superoxide dismutase (SOD) [(68.36±10.65) U/mL vs. (92.84±12.89) U/mL] were obviously lower in the NAFLD group than those in the control group (t=9.11, 11.19, 13.79, 13.98, P<0.001). The levels of lncRNA HCG18 [(1.20±0.25) vs. (1.39±0.30) vs. (1.65±0.35)] and MDA [(5.64±1.88) μmol/L vs. (7.12±2.16) μmol/L vs. (8.99±2.51) μmol/L] in the exclusion of ad?vanced fibrosis group, the intermediate state group and the advanced fibrosis group were obviously increased in turn (F=12.36, 13.06, P <0.001), while the levels of GSH-Px (81.56±13.58) U/mL vs. (71.74±10.52) U/mL vs. (57.06±9.05) U/mL] and SOD [(81.45±12.52) U/ mL vs. (66.52±10.25) U/mL vs. (49.13±8.29) U/mL] were obviously decreased in turn (F=24.27, 48.42, P<0.001). Pearson correlationanalysis results showed that the level of serum lncRNA HCG18 in NAFLD group was positively correlated with MDA (r=0.59, P< 0.001), and negatively correlated with GSH-Px and SOD (r=?0.57, ?0.62, P<0.001). Spearman correlation analysis results showed thatthe level of serum lncRNA HCG18 was positively correlated with NFS score in NAFLD group (r=0.69, P<0.001). The area under the ROC curve (AUC) of serum lncRNA HCG18 for the diagnosis of NAFLD was 0.85.Conclusion Serum lncRNA HCG18 is obviouslyincreased in NAFLD patients, which has a certain correlation with oxidative stress indicators and the degree of liver fibrosis. |
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