| 崔晓佳,谭程,杨百霞,等.白藜芦醇通过阻断 JAK激酶 2/信号转导及转录活化因子 3信号通路抑制食管癌细胞增殖和迁移并诱导其凋亡的作用研究[J].安徽医药,2024,28(6):1103-1108. |
| 白藜芦醇通过阻断 JAK激酶 2/信号转导及转录活化因子 3信号通路抑制食管癌细胞增殖和迁移并诱导其凋亡的作用研究 |
| Effect of resveratrol on inhibiting proliferation, migration and inducing apoptosis of esophageal cancer cells by blocking JAK kinase 2/ signal transduction and transcription activation factor 3 signaling pathway |
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| DOI：10.3969/j.issn.1009-6469.2024.06.009 |
| 中文关键词: 食管肿瘤 白藜芦醇 JAK激酶 2/信号转导及转录活化因子 3信号通路 增殖 凋亡 迁移 |
| 英文关键词: Esophageal neoplasms Resveratrol Janus kinase 2/signal transducer and activator of transcription 3 signaling path way Proliferation Apoptosis Migration |
| 基金项目:2018年度南通市市级科技计划(指导性)项目( MSZ18127)； 2022年南通市卫生健康委员会科研课题( MSZ2022026) |
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| 中文摘要: |
| 目的探究白藜芦醇对人食管癌细胞增殖、凋亡、迁移及 JAK激酶 2/信号转导及转录活化因子 3(JAK2/STAT3)信号通路的调控作用。方法 2021年 7月至 2022年 7月，体外培养人食管癌 OE19细胞，将细胞分为对照组(不做干预)和实验组( 15、 30、60、90和 120 μmol/L白藜芦醇干预 24 h)进行预实验，根据细胞计数试剂盒( CCK-8)预实验结果，筛选有显著作用且细胞活力高于 50%的 30、60、90 μmol/L白藜芦醇进行后续的实验，后续实验又分为对照组(不做干预)、 30、60、90 μmol/L白藜芦醇组(30、60和 90 μmol/L白藜芦醇)和 30 μmol/L白藜芦醇 +抑制剂组( 30 μmol/L白藜芦醇 +10 μmol/L JAK2/STAT3通路抑制剂 AG490)，干预 24 h。用 5-乙炔基 -2'脱氧尿嘧啶核苷( EdU)、 Hoechst 33258染色、 Transwell、实时荧光定量 PCR(RT-qPCR)及蛋白质印迹法对细胞增殖率、凋亡情况、迁移数及细胞周期蛋白 D1(cyclin D1)、胱天蛋白酶 -3(caspase-3)和 JAK2/STAT3相关因子表达水平进行分析。结果不同浓度实验组的细胞活力与对照组相比逐渐降低，其中 30、60、90和 120 μmol/L白藜芦醇差异有统计学意义( P<0.05)但 120 μmol/L白藜芦醇组细胞活力低于 50%，所以本研究选择 30、60和 90 μmol/L白藜芦醇继续后续实验； 60 μmol/L白藜芦醇组，细胞增殖率(41.49±5.06)%、迁移细胞数( 36.67±2.52)个显著低于对照组( 53.34±1.99)%、(58.00± |
| 英文摘要: |
| Objective To explore the regulatory effects of resveratrol on the proliferation, apoptosis, migration and JAK kinase 2/ signal transduction and transcriptional activation factor 3 (JAK2/STAT3) signaling pathway of human esophageal cancer cells.Methods From July 2021 to July 2022, human esophageal cancer OE19 cells were cultured in vitro, and the cells were divided into control group(without intervention) and experimental group (15, 30, 60, 90 and 120 μmol/L resveratrol intervention for 24 h) for pre-experiment. Ac cording to the pre-experiment results of cell counting kit 8 (CCK-8), 30, 60, 90 μmol/L resveratrol with significant effect and cell viability higher than 50% were screened for subsequent experiments. Follow-up experiments were divided into control group (no intervention), 30, 60, 90 μmol/L resveratrol group (30, 60 and 90 μmol/L resveratrol) and 30 μmol/L resveratrol + inhibitor group (30 μmol/Lresveratrol +10 μmol/L) JAK2/STAT3 pathway inhibitor AG490) was treated for 24 h. Cell proliferation rate, apoptosis, migration andcyclin D1 caspase-3 and JAK2/ STAT3-related factors expression were measured by 5-acetylidene-2 'deoxyuracil (EdU), Hoechst 33258 staining, Transwell, RT-qPCR and Western blotting.Results Compared with the control group, the cell viability of the experimental group with different concentrations decreased gradually, and the difference of 30, 60, 90 and 120 μmol/L resveratrol was statistically significant (P<0.05), but the cell viability of 120 μmol/L resveratrol group was lower than 50%. Therefore, 30, 60 and 90 μmol/Lresveratrol was selected for subsequent experiment in this study. The cell proliferation rate (41.49±5.06)% and the number of migratingcells (36.67±2.52) in 60 μmol/L resveratrol group were significantly lower than those in control group (53.34±1.99)% and (58.00±2.00).The number of apoptotic cells (7.67±1.53) was significantly higher than that in control group (2.50±0.71), and the expressions of cyclinD1, p-JAK2 and p-STAT3 in 60 μmol/L resveratrol group were lower than those in control group, and the mRNA and protein expression levels of caspase-3 were higher than those in control group (P<0.05). The above indexes in the 30 and 90 μmol/L resveratrol groupswere similar to those in the control group. Compared with 30 μmol/L resveratrol group, the above indexes in 30 μmol/L resveratrol + inhibitor group had more significant changes (P<0.05). Conclusion Resveratrol can inhibit the proliferation and migration of humanOE19 cells and induce apoptosis by inhibiting the JAK2/STAT3 pathway. |
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