| 何遂庆,吴芳丽,李爱珍.高效液相色谱法快捷测定黄原胶中 D-甘露糖含量的探究[J].安徽医药,2024,28(6):1109-1111. |
| 高效液相色谱法快捷测定黄原胶中 D-甘露糖含量的探究 |
| Study on rapid content determination of D-mannose in Xanthan gum by HPLC |
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| DOI：10.3969/j.issn.1009-6469.2024.06.010 |
| 中文关键词: 黄原胶 D-甘露糖 含量测定 高效液相色谱 质量控制 |
| 英文关键词: Xanthan gum D-mannose Content determination HPLC Quality control |
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| 中文摘要: |
| 目的建立高效液相色谱 -示差折光( HPLC-RID)法测定黄原胶中 D-甘露糖含量的方法。方法该研究起止时间为 2022年 6月至 2023年 3月。选择 Phenomenex Rezex RCM-Monosaccharide Ca(+300 mm×7.80 mm，8 μm)色谱柱，以双蒸水为流动相，流速: 0.4 mL/min，柱温: 75 ℃，进样量: 20 μL，检测器为示差折光检测器( RID)。结果色谱图显示 D-甘露糖分离度良好， D-甘露糖在 20.09~100.46 μg范围内与峰面积线性关系良好( r=0.999 6)D-甘露糖平均加样回收率为 99.10%，相对标准偏差( RSD)为 0.81%(n=6)，精密度、稳定性、重复性试验的 RSD均小于 2%。结论，所建立的 D-甘露糖含量测定方法可靠，操作简便，专属性强，重复性好，适合黄原胶中 D-甘露糖的含量测定，为黄原胶食用以及药用产品的进一步开发提供了参考数据。 |
| 英文摘要: |
| Objective To establish a method for the content determination of D-mannose in Xanthan gum by high performance liquid chromatography differential refraction (HPLC-RID).Methods The study started from June 2022 and ended in March 2023. The analy sis was performed on Phenomenex Rezex RCM-Monosaccharide Ca+ chromatographic column (300 mm×7.80 mm,8 μm), using doubledistilled water as the mobile phase, with a flow rate of 0.4 mL/min, column temperature of 75 ℃, and injection volume of 20 μL. Thetests were carried out by RID.Results The HPLC profile indicated well separation of D-mannose. The calibration curves were linear within the range of 20.09-100.46 μg for D-mannose (r=0.999 6). The average recovery rate was 99.10% with RSD of 0.81% (n=6). The RSDs of precision, stability and repeatability tests did not exceed 2%.Conclusions The established method for the determination of D-mannose content is reliable. The method is simple, specific and reproducible, which can be used to determine the content determination of D-mannose in Xanthan gum. This study provides reference for the further development of Xanthan gum medicinal and edible products. |
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