文章摘要
刘华丽,李娜,高炜熙.一株球毛壳霉次级代谢产物及其体外抗肿瘤活性研究[J].安徽医药,2024,28(7):1307-1312.
一株球毛壳霉次级代谢产物及其体外抗肿瘤活性研究
Study on the secondary metabolites of a strain of Chaetomium globosum and their antitumor activities in vitro
  
DOI:10.3969/j.issn.1009-6469.2024.07.008
中文关键词: 毛壳菌属  球毛壳霉  次级代谢产物  抗肿瘤活性  天然产物  结构鉴定
英文关键词: Chaetomium  Chaetomium globosum  Secondary metabolites  Antitumor activity  Natural products  Structure elucidation
基金项目:国家自然科学基金项目( 82003625;32200706)
作者单位E-mail
刘华丽 武汉大学人民医院 肿瘤中心湖北武汉 430060  
李娜 武汉大学人民医院 肿瘤中心湖北武汉 430060  
高炜熙 武汉大学人民医院药学部湖北武汉 430060 gaoweixi@whu.edu.cn 
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中文摘要:
      目的研究球毛壳霉次级代谢产物及其体外抗肿瘤活性。方法 2021年 3—12月,采用硅胶柱色谱法、凝胶柱色谱法、半制备高效液相色谱法等分离方法和技术对球毛壳霉次级代谢产物进行分离纯化,使用 NMR核磁数据及质谱数据分析鉴定化合物的结构;采用 MTT法评价化合物的体外抗肿瘤活性。结果从球毛壳霉次级代谢产物中分离得到 7个化合物,分别鉴定为( 1)cyclo-(L-Leu-L-Trp),(2)N-acetyl-L-tryptophan,(3)N-acetyltryptamine,(4)armochaetoglobin G,(5)armochaetoglobin J,(6)armochaetoglobin K,(7)chaetoglobosin U;化合物 1和 3为首次从球毛壳霉中分离得到;化合物 4和 5对选定的人肿瘤细胞具有一定的体外抗增殖活性, IC50:12.58~35.28 μmol/L。结论球毛壳霉能够产生结构丰富的代谢产物,其中一些代谢产物有良好的抗肿瘤活性,具有潜在药用价值,其活性化合物有待进一步挖掘。
英文摘要:
      Objective To study the secondary metabolites of Chaetomium globosum and their antitumor activities in vitro.Methods The study started and ended from March 2021 to December 2021. Silica gel column chromatography,gel column chromatography,semipreparative HPLC and other separation methods and techniques were used to separate and purify the chemical components of the secondary metabolites of Chaetomium globosum. NMR and MS data analysis were used to identify the structure of the compounds. The invitro antitumor activity of the compound was evaluated by the MTT method.Results Seven compounds were isolated from the secondary metabolites of Chaetomium globosum,and they were identified as (1) cyclo-(L-Leu-L-Trp),(2) N-acetyl-L-tryptophan, (3) N-acetyltryptamine,(4) armochaetoglobin G,(5) armochaetoglobin J,(6) armochaetoglobin K,(7) chaetoglobosin U. Compounds 1 and 3 were isolated from Chaetomium globosum for the first time. Compounds 4 and 5 displayed varying degrees of in vitro antitumor activities against human tumor cells with IC50: 12.58~35.28 μmol/L.Conclusion Chaetomium globosum can produce metabolites with rich structuraltypes,some of which have good antitumor activity and potential medicinal value,therefore its active metabolites need to be further explored.
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